Difference between revisions of "Team:HZAU-China/WetLab/Design"

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       <h1></br></br></br>Overview</h1></br>
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       <h1></br></br></br>Design</h1></br>
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       <p>As our team is competing this year's competition, we also required to participate in the iGEM 2015 Measurement Interlab study. The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around world.</p>
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       </br><p>The three genetic devices differ in their promotors :</p>
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       <p>It is based on quorum sensing. The luxI protein generates AHL , it’s a signal molecule. In the presence of luxR, the complex can activate the promoter ,the aiia protein can degrade the AHL, repressing the promoter indirect, thus forming a oscillation.</p>
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      <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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      <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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      <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p></br>
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      <p>We transformed the three devices into competent cell (DH 5 alpha) and measured fluorescence as well as optical density of the LB medium at the suitable period (We did pre-experiment before formal experiment for confirming the suitable period as well as the dilution factor), using a plate reader, respectively. We hypothesized that the GFP expression quality of the three devices level is Device 1, Device 2, Device 3, respectively. And our final result accord with our hypothesis.</p>
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Revision as of 19:01, 14 September 2015

Mixed-Reality Cell

Bidirectinal coupling between real and virtual bio-oscillator




Design


It is based on quorum sensing. The luxI protein generates AHL , it’s a signal molecule. In the presence of luxR, the complex can activate the promoter ,the aiia protein can degrade the AHL, repressing the promoter indirect, thus forming a oscillation.




© 2015 Huazhong Agricultural University iGEM Team. All rights reserved.

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