Difference between revisions of "Team:San Andres/Software"

 
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   <!--navigation menu -->
 
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   <br>
 
   <br>
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 +
          <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>
 +
        </td>
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        <td style="border: 3px solid black;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
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          <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
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        </td>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project</a>
 +
        </td>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
 +
        </td>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Modeling</a>
 +
        </td>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"><a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
 +
        </td>
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      </tr>
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    </tbody>
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 +
          <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);">Notebook</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Attributions" style="color: rgb(0, 0, 0);"> Attributions</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 
   <div class="gwd-div-2ed9"></div>
 
   <div class="gwd-div-2ed9"></div>
 
   <div class="gwd-div-7xzo gwd-a-1thu">
 
   <div class="gwd-div-7xzo gwd-a-1thu">
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     <h1>Project&nbsp;</h1>
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     <h1>Abstract</h1>
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     <big><span class="gwd-span-3t71"><span class="gwd-span-fn2e">Our project is about&nbsp;</span><span class="gwd-span-a1dh"><span class="gwd-span-6ea3" lang="EN-US"><span style="">celiac</span></span>
+
     <div style="text-align: justify;"><big>The celiac
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<span class="gwd-span-xrze"><span class="gwd-span-bh3g" lang="EN-US">&nbsp;</span><span class="gwd-span-1fs0"><span class="gwd-span-5vwr" lang="EN-US"><span style="">disease, this is a
+
disease affects 1 in 265 people worldwide and 15%
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enteropathy, autoimmune affecting individuals who possess genes HLA-DQ8
+
of population in Chile. This in an irreversible autoimmune disease in
−
or
+
which due to the presence of genes HLA DQ2 or HLA DQ8 in those
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HLA-DQ2, where T cells attack the body when the gliadins and glutenins
+
affected, the consumption of foods containing gluten (all fods derived
−
are
+
from wheat, barley, rye, etc.) cause an inflammation in small intestine
−
absorbed by the small intestine, in others words, celiac patients have
+
causing multiple effects in the organism (diarrhea, constipation, porr
−
a
+
absorption, among others) significantly affecting their quatily of life.</big>
−
permanent intolerance to gluten-containing foods.</span></span></span><span class="gwd-span-1haj" lang="EN-US"></span></span></span></span></big><big><span style="" lang="EN-US"> <br>
+
−
<br>
+
−
A strict
+
−
diet throughout life gluten-free allows to the small intestine get
+
−
health.
+
−
Obvious sources of gluten are bread, many cereals for breakfast, pasta,
+
−
pizza,
+
−
cakes and biscuits. The gluten can also be used to give structure to
+
−
the food
+
−
products, such as sausages, stock cubes, soups and sauces. Naturally
+
−
the
+
−
gluten-free foods include meat, fish, eggs, fruits, vegetables, dairy
+
−
products,
+
−
beans, potatoes, rice, corn, quinoa and buckwheat. Food substitutes as
+
−
the
+
−
especially gluten-free<span style="">&nbsp; </span>bread,
+
−
flour,
+
−
pasta and biscuits, have increased in recent years in variety and are
+
−
available
+
−
by prescription in some countries.<br>
+
−
<br>
+
−
</span></big>
+
−
    <p style="text-align: left;" class="MsoNormal"><big><span style="" lang="EN-US"><o:p></o:p></span></big><span style="" lang="EN-US"><big>But what
+
−
makes this a problem?, well the problem is the cost of this diet, which
+
−
often
+
−
are priced high in gluten-free foods, besides the clear deprivation the
+
−
patient
+
−
celiac without meals as common as pizza, and even drinks such as beer.
+
−
So we
+
−
think of a way to solve the gap between celiac patients and those
+
−
persons
+
−
without eating disorders, but without forgetting the price, therefore,
+
−
we also
+
−
consider the low cost of production and, like that, was born the idea
+
−
of
+
−
creating a pill that is able to degrade gluten before its contact with
+
−
the
+
−
enterocytes.</big></span>
+
 
       <br>
 
       <br>
−
    </p>
 
−
    <br>
 
−
    <div style="text-align: center;">
 
−
      <img alt="File:Cientific explain.jpg" src="https://static.igem.org/mediawiki/2015/thumb/f/f6/Cientific_explain.jpg/716px-Cientific_explain.jpg" height="600" width="716">
 
 
       <br>
 
       <br>
 +
      <big>Whereas the majority of the world food contains gluten, we
 +
propose a
 +
treatment for those who can't eat gluten, and can do so without
 +
problems. In detail, our project involves a system able to degrade
 +
gluten via the enzyme KumaMax and report the operation simultaneously
 +
with a red fluorescent protein. The foregoing, works in a genetically
 +
modified E. coli bacteria. With this project, in long run, we hope to
 +
improve the quality of life of all those reported with celiac disease.
 +
People who suffer from gluten intolerance have an adverse reaction to
 +
gluten proteins found in wheat, barley, and rye products.</big>
 
       <br>
 
       <br>
−
      <p style="text-align: left;" class="MsoNormal"><big><span style="" lang="EN-US">When the
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    </div>
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gluten enter in contact with the villi of the small intestine
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    <big><br>
−
(enterocytes) the
+
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helper T cells (type of reporter) detects the gluten as a toxic
+
−
substance and
+
−
leaves a chemical trail, which is followed by antibodies, among the
+
−
more
+
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important<span style="">&nbsp; </span>the
+
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Transglutaminase and the
+
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Antigliadin (in this case); In addition to the lymphocytes T and B. The
+
−
first (
+
−
lymphocytes T) would be the natural killers and an army of these cells
+
−
will
+
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attack to the infected cells, which could cause a cancerous effect, in
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this
+
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case the dangerous cells are the enterocytes, and after of the atack
+
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they could
+
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causing inflammation, poor absorption, and other symptoms. But does not
+
−
stop
+
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there the auto-immune action, also the B cells serves as a kind of
+
−
memory which
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is used to the next time that the threat enter in the body, the reply
+
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will be
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more quickly and efficiently.<o:p></o:p></span></big>
+
−
      </p>
+
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      <div style="text-align: left;"><big>
+
 
</big>
 
</big>
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        <br>
 
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      </div>
 
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    </div>
 
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    <span class="gwd-span-3t71"><span class="gwd-span-a1dh"><span class="gwd-span-xrze"><span class="gwd-span-1haj" lang="EN-US">
 
−
</span></span>
 
−
    </span>
 
−
    </span>
 
 
     <div style="text-align: center;">
 
     <div style="text-align: center;">
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       <img src="https://static.igem.org/mediawiki/2015/4/44/Symptoms_of_celiac_disease.jpg" class="gwd-img-c7yq">
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       <img style="width: 893px; height: 373px;" alt="File:Ex 2.jpg" src="https://static.igem.org/mediawiki/2015/thumb/9/93/Ex_2.jpg/800px-Ex_2.jpg">
−
      <br>
+
−
      <br>
+
−
      <div style="text-align: left;"><span class="gwd-span-3t71"><span class="gwd-span-u82g"><big>The
+
−
damage caused by the autoimmune reaction can cause
+
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histological&nbsp;damage in the villi, and a biopsy of these is the
+
−
means of diagnosis of this pathology. The only current treatment is a
+
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diet without gluten, which is expensive and difficult to follow.</big><br>
+
−
<br>
+
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</span></span>
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        <div style="text-align: center;"><span class="gwd-span-3t71"><span class="gwd-span-u82g">&nbsp;</span></span>
+
−
          <img src="https://static.igem.org/mediawiki/2015/0/0a/Histological_study.jpg" class="gwd-img-5j7g">
+
−
          <br>
+
−
        </div>
+
−
        <br>
+
−
        <span class="gwd-span-3t71">
+
−
<div class="gwd-div-rxi8"><big><span class="gwd-span-oxe1">This is the tip of the iceberg of
+
−
celiac disease, the symptoms as are easy to diagnose, but there are
+
−
other four ways where the symptoms are not so clear. These forms are:<br class="gwd-br-q8fj">
+
−
</span>
+
−
        </big>
+
−
        <ol class="gwd-ol-ewzq">
+
−
          <li class="gwd-li-cx4k"><big><span class="gwd-span-bp36">Silent Celiac Disease: Patients have
+
−
no symptoms but if damage histological.</span> </big>
+
−
          </li>
+
−
          <li class="gwd-li-83gw"><big><span class="gwd-span-y410">Latent Celiac Disease: There are not
+
−
symptoms and stunted villi. Individuals tend to develop the disease
+
−
suddenly.</span> </big>
+
−
          </li>
+
−
          <li class="gwd-li-hrrk"><big><span class="gwd-span-9f33">Potential Celiac Disease: Are subjects
+
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with high genetic risk that could have the disease.</span> </big>
+
−
          </li>
+
−
          <li class="gwd-li-7d5y"><big><span class="gwd-span-juom">Refractory Celiac Disease: Those
+
−
patients diagnosted celiac and despite follow a diet without gluten
+
−
doesn't improve and can get diseases even more serious.</span></big>
+
−
          </li>
+
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        </ol>
+
−
        <div style="text-align: center;">
+
−
          <img src="https://static.igem.org/mediawiki/2015/3/37/Iceberg_CD.jpg" class="gwd-img-74jg">
+
−
          <br>
+
−
          <br>
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          <div style="text-align: left;">
+
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            <div style="text-align: left;"><big><span style="" lang="EN-US">The celiac disease are associated with many
+
−
diseases, these can also be complications of refractory patients. But is<span style="">&nbsp; </span>important know the variety
+
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of diseases that
+
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can produces by de celiac disease: <o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""><br>
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<br>
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</span>- Food
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intolerance problems.<br>
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<o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""></span>-
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Variety of cancers.<o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""><br>
+
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</span>-
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Diseases related to the nervous system.<o:p></o:p></span></big>
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              <big><span style="" lang="EN-US"><span style=""><br>
+
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</span>- Diseases related to the circulatory system.<o:p></o:p></span></big><big><span style="" lang="EN-US"><span style=""><br>
+
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</span></span>-
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Skin-related illnesses.</big>
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              <o:p></o:p>
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              <big><span class="gwd-span-3t71"><span class="gwd-span-h4j2"></span></span></big>
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−
              <br>
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              <br>
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            </div>
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            <div style="text-align: center;">
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−
              <img src="https://static.igem.org/mediawiki/2015/c/c3/Complications.jpg" class="gwd-img-4j8q">
+
−
              <br>
+
−
              <br>
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−
              <div style="text-align: left;">
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−
                <h1>Statistics</h1>
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                <div style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; text-align: center; background-color: rgb(255, 255, 255);">
+
−
                  <br class="Apple-interchange-newline">
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−
                  <img src="https://static.igem.org/mediawiki/2015/7/75/Celiaco.jpg" alt="celiaco.jpg" title="celiaco.jpg" style="border: medium none ; vertical-align: middle; float: left; width: 503px; height: 466px;">
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                  <br>
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                  <br>
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our country Chile, the statistics are the following:</big><span style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; display: inline ! important; float: none; background-color: rgb(255, 255, 255);"></span>
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probability of having celiac disease is 1 each 265.</big>
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                  <li style="margin-bottom: 0.1em;"><big>The celiac
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patients represent 15% of the Chilean population.</big>
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                  <li style="margin-bottom: 0.1em;"><big>Most die by
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cancer without really knowing their status.</big>
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But Europe have the contries with more celiacs internationally, also
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Europe it is estimated that the 1% of adults and children have the
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    <div style="text-align: left;"><big style="color: rgb(0, 0, 0); font-family: sans-serif; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: rgb(255, 255, 255);">Since
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the main gene pool of people with this mutation originated in Northern
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Europe, all of their descendents throughout North America, Australia,
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South Africa, etc. are at a higher risk for inheriting this genetic
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predisposition to the disease.&nbsp;</big><span id="result_box" class="" style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: rgb(255, 255, 255);"
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      <span class="hps">of celiac disease</span><span class="Apple-converted-space">&nbsp;</span><span class="hps">by</span><span class="Apple-converted-space">&nbsp;</span><span class="hps">ethnic</span><span class="Apple-converted-space">&nbsp;</span>
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Latest revision as of 01:22, 15 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Abstract

The celiac disease affects 1 in 265 people worldwide and 15% of population in Chile. This in an irreversible autoimmune disease in which due to the presence of genes HLA DQ2 or HLA DQ8 in those affected, the consumption of foods containing gluten (all fods derived from wheat, barley, rye, etc.) cause an inflammation in small intestine causing multiple effects in the organism (diarrhea, constipation, porr absorption, among others) significantly affecting their quatily of life.

Whereas the majority of the world food contains gluten, we propose a treatment for those who can't eat gluten, and can do so without problems. In detail, our project involves a system able to degrade gluten via the enzyme KumaMax and report the operation simultaneously with a red fluorescent protein. The foregoing, works in a genetically modified E. coli bacteria. With this project, in long run, we hope to improve the quality of life of all those reported with celiac disease. People who suffer from gluten intolerance have an adverse reaction to gluten proteins found in wheat, barley, and rye products.

File:Ex 2.jpg