Difference between revisions of "Team:San Andres/Software"

 
(54 intermediate revisions by the same user not shown)
Line 2: Line 2:
  
 
<head data-gwd-animation-mode="quickMode">
 
<head data-gwd-animation-mode="quickMode">
 +
  <title>&lt;endnote&gt;&lt;head&gt; &lt;title&gt;&lt;endnote&gt;&lt;head&gt; &lt;meta&gt; &lt;title&gt;
 +
  </title>
 +
</head>
 +
 +
<body>
 +
  <title>wiki</title>
 +
  <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 +
  <meta name="generator" content="Google Web Designer 1.3.2.0521">
 +
  <style type="text/css">
 +
    html, body {
 +
      width: 100%;
 +
      height: 100%;
 +
      margin: 0px;
 +
    }
 +
    body {
 +
      -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 +
      -webkit-transform-style: preserve-3d;
 +
      background-image: url(https://static.igem.org/mediawiki/2015/8/8a/BBD7.jpg);
 +
      background-color: rgb(198, 169, 117);
 +
    }
 +
  </style>
 +
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 +
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 +
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 
   <title>wiki</title>
 
   <title>wiki</title>
 
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
Line 17: Line 44:
 
     }
 
     }
 
   </style>
 
   </style>
−
</head>
 
−
 
−
<body class="gwd-body-kwce">
 
 
   <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 
   <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 
   <title></title>
 
   <title></title>
Line 128: Line 152:
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
 
       background-color: transparent;
 
       background-color: transparent;
 +
    }
 +
    .gwd-table-nb4m {
 +
      font-size: 24px;
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 +
      background-image: none;
 +
      background-color: rgb(255, 255, 255);
 
     }
 
     }
 
     .gwd-td-b6db {
 
     .gwd-td-b6db {
Line 508: Line 539:
 
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0.05, 0, 0, 1);
 
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0.05, 0, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
−
    }
 
−
    .gwd-img-l2p5 {
 
−
      position: absolute;
 
−
      width: 135px;
 
−
      left: -160px;
 
−
      top: 26px;
 
 
     }
 
     }
 
     .gwd-img-n53q {
 
     .gwd-img-n53q {
Line 534: Line 559:
 
     }
 
     }
 
     .gwd-a-36ww {
 
     .gwd-a-36ww {
−
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
+
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, -16.1, 0.05, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
 
     }
 
     }
Line 545: Line 570:
 
       top: 349.384236453212px;
 
       top: 349.384236453212px;
 
       width: 250px;
 
       width: 250px;
 +
    }
 +
    .gwd-img-zpsn {
 +
      position: absolute;
 +
      width: 150px;
 +
      left: -165.73913043478px;
 +
      top: 49.3260869565235px;
 +
    }
 +
    .gwd-a-d01x {
 +
      -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 +
      -webkit-transform-style: preserve-3d;
 +
    }
 +
    .gwd-table-10nh {
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 +
    }
 +
    .gwd-table-15ao {
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 
     }
 
     }
 
   </style>
 
   </style>
 
   <!--navigation menu -->
 
   <!--navigation menu -->
 
   <br>
 
   <br>
 +
  <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-15ao">
 +
    <tbody>
 +
      <tr heigth="15px">
 +
      </tr>
 +
      <tr heigth="75px">
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>
 +
        </td>
 +
        <td style="border: 3px solid black;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Modeling</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"><a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
  <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-10nh">
 +
    <tbody>
 +
      <tr heigth="15px">
 +
      </tr>
 +
      <tr heigth="75px">
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);">Notebook</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Attributions" style="color: rgb(0, 0, 0);"> Attributions</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 
   <div class="gwd-div-2ed9"></div>
 
   <div class="gwd-div-2ed9"></div>
 
   <div class="gwd-div-7xzo gwd-a-1thu">
 
   <div class="gwd-div-7xzo gwd-a-1thu">
−
     <h1>Parts&nbsp;</h1>
+
     <h1>Abstract</h1>
−
     <big>Throughout the project we started to learn the fundamental
+
     <div style="text-align: justify;"><big>The celiac
−
principles of synthetic biology to get to work on our plasmid with
+
disease affects 1 in 265 people worldwide and 15%
−
which we want to see gluten degradation via the enzyme Kumamax. For
+
of population in Chile. This in an irreversible autoimmune disease in
−
this we going to insert in an e. coli the parts (Biobricks) needed to
+
which due to the presence of genes HLA DQ2 or HLA DQ8 in those
−
make our future bacteria can degrade gluten. The parts are:<br>
+
affected, the consumption of foods containing gluten (all fods derived
−
</big>
+
from wheat, barley, rye, etc.) cause an inflammation in small intestine
−
    <ul>
+
causing multiple effects in the organism (diarrhea, constipation, porr
−
      <li><big><span style="font-family: 'Arial','sans-serif';"><strong style="font-weight: bold;">Promoter
+
absorption, among others) significantly affecting their quatily of life.</big>
−
    <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a href="http://parts.igem.org/Part:BBa_J23119"><span style="font-weight: bold;">)</span></a>:
+
       <br>
−
    </span>Constitutive
+
−
promoter (which works permanently) that is give in the relative
+
−
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
+
−
      </li>
+
−
      <li><big><strong style="font-weight: bold;">RBS</strong><strong style="font-weight: bold;">
+
−
    <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
+
−
Synthetic RBS with uplifting sequence</big>.</li>
+
−
      <li><big><strong>Vector:<span style="font-weight: normal;"> <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
+
−
      </li>
+
−
      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Coding Region:
+
−
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
+
−
It
+
−
degrades gluten,
+
−
celiac disease leading cause. Enzyme generated by rational mutation for
+
−
the active site of it. It was created by the team IGEM <a href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
+
−
2011.</a></big>
+
−
      </li>
+
−
      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Reporter: RFP
+
−
    <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span style="font-family: Arial,sans-serif;"> Red f</span>luorescence
+
−
protein.</big>
+
−
      </li>
+
−
      <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
+
−
    <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
+
−
    </span>Dual terminator consisting of
+
−
the B0010 and B0012 parties. It serves to give greater efficiency in
+
−
transcription</big>.</li>
+
−
    </ul>
+
−
    <div style="text-align: center;">
+
−
       <img alt="File:Parts.jpg" src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg" height="59" width="288">
+
 
       <br>
 
       <br>
−
       <img alt="File:Circuito.jpg" src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg" height="309" width="800">
+
       <big>Whereas the majority of the world food contains gluten, we
 +
propose a
 +
treatment for those who can't eat gluten, and can do so without
 +
problems. In detail, our project involves a system able to degrade
 +
gluten via the enzyme KumaMax and report the operation simultaneously
 +
with a red fluorescent protein. The foregoing, works in a genetically
 +
modified E. coli bacteria. With this project, in long run, we hope to
 +
improve the quality of life of all those reported with celiac disease.
 +
People who suffer from gluten intolerance have an adverse reaction to
 +
gluten proteins found in wheat, barley, and rye products.</big>
 
       <br>
 
       <br>
−
      <div style="text-align: left;"><big>This is a
 
−
graphic model of as it has be our plasmid where we can visualize the
 
−
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
 
−
joined by means of prefixes and suffixes that indicate the locations of
 
−
court.</big>
 
−
        <br>
 
−
        <br>
 
−
        <div style="text-align: center;">
 
−
          <img alt="File:Plasmido.jpg" src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg" height="600" width="664">
 
−
        </div>
 
−
      </div>
 
 
     </div>
 
     </div>
 
     <big><br>
 
     <big><br>
 
</big>
 
</big>
 +
    <div style="text-align: center;">
 +
      <img style="width: 893px; height: 373px;" alt="File:Ex 2.jpg" src="https://static.igem.org/mediawiki/2015/thumb/9/93/Ex_2.jpg/800px-Ex_2.jpg">
 +
    </div>
 +
    <p class="MsoListParagraph" style="text-indent: -18pt;">
 +
      <br>
 +
    </p>
 +
    <p class="MsoListParagraph" style="text-indent: -18pt;"></p>
 
   </div>
 
   </div>
 
   <br>
 
   <br>
 
   <a href="https://2015.igem.org/Main_Page" class="gwd-a-3xpx gwd-a-36ww">
 
   <a href="https://2015.igem.org/Main_Page" class="gwd-a-3xpx gwd-a-36ww">
−
     <img style="border: 0px solid ; width: 150px; height: 150px; top: 35px; left: 989px;" alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" class="gwd-img-kwqf">
+
     <img style="border: 0px solid ; width: 150px; height: 150px; left: 989px; top: 49px;" alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" class="gwd-img-kwqf">
 
   </a>
 
   </a>
−
   <a href="https://igem.org/Team_List?year=2015" class="gwd-a-3xpx">
+
   <a href="https://igem.org/Team_List?year=2015" class="gwd-a-3xpx gwd-a-d01x">
−
     <img src="https://static.igem.org/mediawiki/2015/thumb/9/94/Logo43.png/454px-Logo43.png" class="gwd-img-l2p5">
+
     <img src="https://static.igem.org/mediawiki/2015/thumb/8/85/Logo_Definitvo.png/654px-Logo_Definitvo.png" class="gwd-img-zpsn">
 
   </a>
 
   </a>
 
   <br>
 
   <br>
 
   <br>
 
   <br>
−
   <div class="gwd-div-t5nw gwd-div-fola">
+
   <br>
−
    <style>
+
  <br>
−
      body {
+
   <br>
−
        font-family: Arial;
+
−
      }
+
−
      nav ul {
+
−
        list-style: none;
+
−
        padding: 0px;
+
−
      }
+
−
      nav li {
+
−
        line-height: 3rem;
+
−
        position: relative;
+
−
        width: 190px;
+
−
      }
+
−
      nav li ul {
+
−
        position: absolute;
+
−
      }
+
−
      nav a {
+
−
        color: rgb(0, 0, 0);
+
−
        display: block;
+
−
        padding: 0px 2.5em;
+
−
        text-decoration: none;
+
−
        transition: 0.3s;
+
−
        -webkit-transition: 0.3s;
+
−
      }
+
−
      nav a:hover {
+
−
        background: rgb(255, 186, 0);
+
−
      }
+
−
      nav li ul {
+
−
        display: none;
+
−
      }
+
−
      nav li:hover ul {
+
−
        display: block;
+
−
      }
+
−
      nav > ul {
+
−
        display: table;
+
−
        width: 100%;
+
−
        background: rgb(255, 166, 0);
+
−
      }
+
−
      nav > ul > li {
+
−
        float: left;
+
−
      }
+
−
      nav li li {
+
−
        max-height: 0px;
+
−
        transition: all 0.1s;
+
−
        -webkit-transition: all 0.1s;
+
−
        overflow: hidden;
+
−
        width: 200px;
+
−
        background: rgb(237, 173, 0);
+
−
      }
+
−
      nav li:hover > ul > li {
+
−
        max-height: 500px;
+
−
        overflow: visible;
+
−
      }
+
−
      nav > ul > li > ul {
+
−
        top: 100%;
+
−
      }
+
−
      .gwd-nav-nvls {
+
−
        -webkit-transform: translate3d(-13.57px, 349.01px, 0px);
+
−
        -webkit-transform-style: preserve-3d;
+
−
      }
+
−
      .gwd-div-fola {
+
−
        -webkit-transform: translate3d(-7.67px, -1793.66px, 0px);
+
−
        -webkit-transform-style: preserve-3d;
+
−
      }
+
−
    </style>
+
−
    <nav class="gwd-nav-nvls">
+
−
      <ul>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres">Home</a>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Team">Team</a>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Description">Project</a>
+
−
          <ul>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Parts">Parts</a>
+
−
            </li>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Modeling">Modeling</a>
+
−
            </li>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Results">Results</a>
+
−
            </li>
+
−
          </ul>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Notebook">Notebook</a>
+
−
          <ul>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Practices">Human Practices</a>
+
−
            </li>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Design">Future Projections</a>
+
−
            </li>
+
−
          </ul>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Collaborations">Collaborations</a>
+
−
        </li>
+
−
      </ul>
+
−
    </nav>
+
−
   </div>
+
 
</body>
 
</body>
  
 
</html>
 
</html>

Latest revision as of 01:22, 15 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Abstract

The celiac disease affects 1 in 265 people worldwide and 15% of population in Chile. This in an irreversible autoimmune disease in which due to the presence of genes HLA DQ2 or HLA DQ8 in those affected, the consumption of foods containing gluten (all fods derived from wheat, barley, rye, etc.) cause an inflammation in small intestine causing multiple effects in the organism (diarrhea, constipation, porr absorption, among others) significantly affecting their quatily of life.

Whereas the majority of the world food contains gluten, we propose a treatment for those who can't eat gluten, and can do so without problems. In detail, our project involves a system able to degrade gluten via the enzyme KumaMax and report the operation simultaneously with a red fluorescent protein. The foregoing, works in a genetically modified E. coli bacteria. With this project, in long run, we hope to improve the quality of life of all those reported with celiac disease. People who suffer from gluten intolerance have an adverse reaction to gluten proteins found in wheat, barley, and rye products.

File:Ex 2.jpg