Difference between revisions of "Team:San Andres/Software"

 
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 +
          <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>
 +
        </td>
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        <td style="border: 3px solid black;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
 +
        </td>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project</a>
 +
        </td>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
 +
        </td>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Modeling</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"><a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
 +
        </td>
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      </tr>
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 +
          <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);">Notebook</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Attributions" style="color: rgb(0, 0, 0);"> Attributions</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 
   <div class="gwd-div-2ed9"></div>
 
   <div class="gwd-div-2ed9"></div>
 
   <div class="gwd-div-7xzo gwd-a-1thu">
 
   <div class="gwd-div-7xzo gwd-a-1thu">
−
     <h1>Enzymes</h1>
+
     <h1>Abstract</h1>
−
     <big>During our investigation we sought the perfect enzyme to
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     <div style="text-align: justify;"><big>The celiac
−
degrade the gluten, and we found:<br>
+
disease affects 1 in 265 people worldwide and 15%
−
</big>
+
of population in Chile. This in an irreversible autoimmune disease in
−
    <ul>
+
which due to the presence of genes HLA DQ2 or HLA DQ8 in those
−
      <li><big>Prolyl Endopeptidase: <span style="" lang="EN-US">The prolyl
+
affected, the consumption of foods containing gluten (all fods derived
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endopeptidase (PEP), is a class of serine-protease able to break
+
from wheat, barley, rye, etc.) cause an inflammation in small intestine
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peptide bonds
+
causing multiple effects in the organism (diarrhea, constipation, porr
−
following a PROLINE residue terminal carboxyl group. Were studying it
+
absorption, among others) significantly affecting their quatily of life.</big>
−
its use
+
−
as a therapeutic agent against celiac disease (CD), characterized by
+
−
atrophy of
+
−
the intestinal villi and inflammation. These reactions are attributed
+
−
to
+
−
peptides rich in PROLINE that are generated during the digestion of
+
−
gluten of
+
−
some cereals. This enzyme could be used as a nutritional supplement for
+
−
individuals who suffer from celiac disease or during the processing of
+
−
starches
+
−
produced from cereals containing gluten. While it was a candidate for a
+
−
possible treatment failed to meet expectations, because its activity is
+
−
to a
+
−
high pH, which is not suitable for an average digestive. Another reason
+
−
was
+
−
that degrades the gluten slowly, which would have resulted in a longer
+
−
and
+
−
inefficient treatment.</span><big>&nbsp;</big></big>
+
−
      </li>
+
 
       <br>
 
       <br>
−
    </ul>
 
−
    <div style="text-align: center;">
 
−
      <img alt="File:Prolil.jpg" src="https://static.igem.org/mediawiki/2015/c/c0/Prolil.jpg" height="376" width="565">
 
 
       <br>
 
       <br>
 +
      <big>Whereas the majority of the world food contains gluten, we
 +
propose a
 +
treatment for those who can't eat gluten, and can do so without
 +
problems. In detail, our project involves a system able to degrade
 +
gluten via the enzyme KumaMax and report the operation simultaneously
 +
with a red fluorescent protein. The foregoing, works in a genetically
 +
modified E. coli bacteria. With this project, in long run, we hope to
 +
improve the quality of life of all those reported with celiac disease.
 +
People who suffer from gluten intolerance have an adverse reaction to
 +
gluten proteins found in wheat, barley, and rye products.</big>
 
       <br>
 
       <br>
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      <div style="text-align: left;">
+
    </div>
−
        <ul>
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    <big><br>
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          <li><big>Kumamolisin As: It is the first known example of
+
−
a collagenase derived from the family of the sedolisin. This operates
+
−
at high temperatures and low pH levels. Its characteristics, together
+
−
with those predicted are measured by comparison between a collagenase
+
−
and a peptidase from serine, which are related to the enzyme
+
−
preference, to thus Digest collagen as gluten.</big>
+
−
          </li>
+
−
        </ul>
+
−
        <div style="text-align: center;">
+
−
          <img alt="File:2-2-2 2.jpg" src="https://static.igem.org/mediawiki/2015/b/be/2-2-2_2.jpg" height="243" width="470">
+
−
          <br>
+
−
          <ul style="text-align: left;">
+
−
            <li><big>KumaMax (G319S, D358G, D368H, N281D): It is a
+
−
mutation of the Kumamolisin As, which is designed to digest way more
+
−
efficient gluten, because that can work at pH levels much more lower
+
−
than the original enzyme (a pH of 4.0) which is excellent for the
+
−
average digestive system. <span class="hps">It was
+
−
created by the team IGEM <a href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
+
−
2011</a></span>. Other advantages
+
−
are:</big>
+
−
            </li>
+
−
          </ul>
+
−
          <ol style="text-align: left;">
+
−
            <li><big>It is resistant to high temperatures and acidity
+
−
of the stomach.</big>
+
−
            </li>
+
−
            <li><big>It is heat stable, in others words, it is
+
−
resistant to all changes in their physical and chemical structure.</big>
+
−
            </li>
+
−
            <li><big>It is easily repairable and creable.</big>
+
−
            </li>
+
−
          </ol>
+
−
          <div style="text-align: center;">
+
−
            <img alt="File:175px-Washington Bottle.jpg" src="https://static.igem.org/mediawiki/2015/d/d0/175px-Washington_Bottle.jpg" height="263" width="175">
+
−
            <img alt="File:250px-Washington Kumamolisin VS SC-PEP.png" src="https://static.igem.org/mediawiki/2015/1/1b/250px-Washington_Kumamolisin_VS_SC-PEP.png" height="213" width="250">&nbsp;
+
−
            <img alt="File:250px-Washington Kuma Bonded triad.png" src="https://static.igem.org/mediawiki/2015/6/62/250px-Washington_Kuma_Bonded_triad.png" height="193" width="250">
+
−
            <br>
+
−
            <br>
+
−
            <h1 style="border-bottom: 1px solid rgb(170, 170, 170); margin: 0px 0px 0.6em; background: transparent none repeat scroll 0% 50%; -moz-background-clip: initial; -moz-background-origin: initial; -moz-background-inline-policy: initial; color: rgb(0, 0, 0); font-weight: normal; padding-top: 0.5em; padding-bottom: 0.17em; font-size: 23.876px; font-family: sans-serif; font-style: normal; font-variant: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px;">Metodology&nbsp;</h1>
+
−
            <div style="text-align: left;"><big style="color: rgb(0, 0, 0); font-family: sans-serif; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px;">The
+
−
methodology consists of:<br>
+
 
</big>
 
</big>
−
              <p class="MsoNormal"><big><span style="" lang="EN-US">- For our
+
    <div style="text-align: center;">
−
Plasmid construction, we decided to take the method standard of
+
      <img style="width: 893px; height: 373px;" alt="File:Ex 2.jpg" src="https://static.igem.org/mediawiki/2015/thumb/9/93/Ex_2.jpg/800px-Ex_2.jpg">
−
Assembly
+
−
biobrick, based on grafts and vectors, front and reverse.<o:p></o:p></span></big>
+
−
              </p>
+
−
              <p class="MsoNormal"><big><span style="" lang="EN-US">- Then we
+
−
cut the two genes and making them again an insert front, which we took
+
−
to the
+
−
vector of the terminator making as well as the genes a front vector.<o:p></o:p></span></big>
+
−
              </p>
+
−
              <p class="MsoNormal"><big><span style="" lang="EN-US">- After
+
−
making two front inserts, we proceed to start with two reverse inserts
+
−
for
+
−
where we cut the three genes (Kumamax, RFP, terminator) and we make
+
−
them a
+
−
reverse insert, and we took him to the inverse vector of the RBS.<o:p></o:p></span></big>
+
−
              </p>
+
−
              <p class="MsoNormal"><span style="" lang="EN-US"><big>-
+
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Finally
+
−
we proceed to cut the four genes (RBS, Kumamax, RFP, terminator) as
+
−
insert
+
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reverse, to take them to the inverse vector of the promoter, and we
+
−
finished
+
−
building our final plasmid, the "Kumamax Plux".</big><o:p></o:p></span>
+
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              </p>
+
−
              <p class="MsoNormal"><span style="" lang="EN-US"><o:p></o:p></span>
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−
              </p>
+
−
            </div>
+
−
            <br style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px;">
+
−
            <div style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; text-align: center;">
+
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              <img class="shrinkToFit" alt="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" src="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" style="border: medium none ; vertical-align: middle;" height="657" width="755">
+
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              <div style="text-align: left;"><big>At this time we
+
−
are innovating ideas to add a circuit that will allow us in the future
+
−
to obtain a method of detecting and quantifying the presence of gluten,
+
−
which can also be checked by a commercial kit.</big>
+
−
                <br>
+
−
                <br>
+
−
                <div style="text-align: center;">
+
−
                  <img alt="File:Kit gluten.jpg" src="https://static.igem.org/mediawiki/2015/0/09/Kit_gluten.jpg" style="border: medium none ; vertical-align: middle;" height="513" width="593">
+
−
                </div>
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              </div>
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            <big style="color: rgb(0, 0, 0); font-family: sans-serif; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px;"><br>
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</big><span style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; display: inline ! important; float: none; background-color: rgb(255, 255, 255);"></span>
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Latest revision as of 01:22, 15 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Abstract

The celiac disease affects 1 in 265 people worldwide and 15% of population in Chile. This in an irreversible autoimmune disease in which due to the presence of genes HLA DQ2 or HLA DQ8 in those affected, the consumption of foods containing gluten (all fods derived from wheat, barley, rye, etc.) cause an inflammation in small intestine causing multiple effects in the organism (diarrhea, constipation, porr absorption, among others) significantly affecting their quatily of life.

Whereas the majority of the world food contains gluten, we propose a treatment for those who can't eat gluten, and can do so without problems. In detail, our project involves a system able to degrade gluten via the enzyme KumaMax and report the operation simultaneously with a red fluorescent protein. The foregoing, works in a genetically modified E. coli bacteria. With this project, in long run, we hope to improve the quality of life of all those reported with celiac disease. People who suffer from gluten intolerance have an adverse reaction to gluten proteins found in wheat, barley, and rye products.

File:Ex 2.jpg