Difference between revisions of "Team:HZAU-China/HardWare"

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       <h1></br></br></br>Overview</h1></br>
 
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       <p>As our team is competing this year's competition, we also required to participate in the iGEM 2015 Measurement Interlab study. The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around world.</p>
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       <p>A genetic oscillator was built in E.coil as the real part while an e-oscillator was simulated in a computer as the virtual part. To realize our idea, two issues should be taken into account. The two parts should have the ability to interact with each other and cells have to keep alive for the long and real-time observation. Thus, an interface device is needed to settle them. This page will demonstrate information about the interface and it can be divided into several parts. Firstly, it’s an introduction and shows the necessity of interface device. Then, the whole design and current progress of device is showed. Finally, we will present other devices using for coupling with the virtual part and lab test. Though we are not professional engineering students and not good at hardware, we keep learning relevant knowledge of hardware during the whole summer. Many factors are considered to make the device more accurate.</p>
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      </br><p>The three genetic devices differ in their promotors :</p>
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      <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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      <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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      <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p></br>
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      <p>We transformed the three devices into competent cell (DH 5 alpha) and measured fluorescence as well as optical density of the LB medium at the suitable period (We did pre-experiment before formal experiment for confirming the suitable period as well as the dilution factor), using a plate reader, respectively. We hypothesized that the GFP expression quality of the three devices level is Device 1, Device 2, Device 3, respectively. And our final result accord with our hypothesis.</p>
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Revision as of 02:11, 15 September 2015

Mixed-Reality Cell

Bidirectinal coupling between real and virtual bio-oscillator




Overview


A genetic oscillator was built in E.coil as the real part while an e-oscillator was simulated in a computer as the virtual part. To realize our idea, two issues should be taken into account. The two parts should have the ability to interact with each other and cells have to keep alive for the long and real-time observation. Thus, an interface device is needed to settle them. This page will demonstrate information about the interface and it can be divided into several parts. Firstly, it’s an introduction and shows the necessity of interface device. Then, the whole design and current progress of device is showed. Finally, we will present other devices using for coupling with the virtual part and lab test. Though we are not professional engineering students and not good at hardware, we keep learning relevant knowledge of hardware during the whole summer. Many factors are considered to make the device more accurate.




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