Difference between revisions of "Team:Tokyo Tech/Experiment/Overview of fim inversion system"
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<h3 class="link"><a href="#Introduction">1. Introduction</a></h3> | <h3 class="link"><a href="#Introduction">1. Introduction</a></h3> | ||
<h3 class="link"><a href="#How">2. How to <i>fim</i> switch works</a></h3> | <h3 class="link"><a href="#How">2. How to <i>fim</i> switch works</a></h3> | ||
− | <h3 class="link2"><a href="#Wild">2.1. Wild type | + | <h3 class="link2"><a href="#Wild">2.1. Wild type <i>fim</i> switch</a></h3> |
− | <h3 class="link3"><a href="# | + | <h3 class="link3"><a href="#tdesign">2.1.1. The design of wild type fimswitch </a></h3> |
<h3 class="link3"><a href="#FimB">2.1.2. Result of FimB/FimE Assay to fimS(TT)</a></h3> | <h3 class="link3"><a href="#FimB">2.1.2. Result of FimB/FimE Assay to fimS(TT)</a></h3> | ||
<h3 class="link3"><a href="#AHL">2.1.3. AHL dependent FimE Assay to fimS(TT)</a></h3> | <h3 class="link3"><a href="#AHL">2.1.3. AHL dependent FimE Assay to fimS(TT)</a></h3> | ||
<h3 class="link2"><a href="#Tokyofim">2.2. Tokyo_Tech <i>fim</i> switch</a></h3> | <h3 class="link2"><a href="#Tokyofim">2.2. Tokyo_Tech <i>fim</i> switch</a></h3> | ||
− | <h3 class="link3"><a href="# | + | <h3 class="link3"><a href="#wdesign">2.2.1. The design of wild type <i>fim</i> switch </a></h3> |
− | <h3 class="link3"><a href="#FimB">2.2.2. The design of Tokyo_Tech <i>fim</i>switch | + | <h3 class="link3"><a href="#FimB">2.2.2. The design of Tokyo_Tech <i>fim</i> switch</a></h3> |
− | + | ||
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<p class="text">ここにコピペ。</p> | <p class="text">ここにコピペ。</p> | ||
− | <h2 id=" | + | <h2 id="How" class="smalltitle">2. How to <i>fim</i> switch works</h2> |
− | + | <h3 id="Wild" class="sub5">2.1. Wild type <i>fim</i> switch</h3> | |
<h2 id="Results" class="smalltitle">3. Results</h2> | <h2 id="Results" class="smalltitle">3. Results</h2> |
Revision as of 03:04, 15 September 2015
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Overview of fim inversion system
We introduce fim system
![](https://static.igem.org/mediawiki/2015/a/a4/Tokyo_Tech_textarea_top.png)
contents
1. Introduction
2. How to fim switch works
2.1. Wild type fim switch
2.1.1. The design of wild type fimswitch
2.1.2. Result of FimB/FimE Assay to fimS(TT)
2.1.3. AHL dependent FimE Assay to fimS(TT)
2.2. Tokyo_Tech fim switch
2.2.1. The design of wild type fim switch
2.2.2. The design of Tokyo_Tech fim switch
3. Results
4. Discussion
5. Materials and Methods
5.1. Construction
5.2. Assay Protocol
5.2.1. Protocol1
5.2.2. Protocol2
6. Reference
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![](https://static.igem.org/mediawiki/2015/a/a4/Tokyo_Tech_textarea_top.png)
1. Introduction
ここにコピペ。
2. How to fim switch works
2.1. Wild type fim switch
3. Results
ここにコピペ。
4. Discussion
ここにコピペ。
5. Materials and Methods
5.1. Construction
-Strain
All the samples were DH5alpha strain.
-Plasmids
Device 1: J23101 + I13504(pSB1C3)
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|
Fig.3-7-4-1. |
5.2. Assay Protocol
5.2.1. Protocol1
1. コピペ。
2. コピペ。
3. コピペ。
4. コピペ。
5. コピペ。
6. コピペ。
7. コピペ。
8. コピペ。
9. コピペ。
6.. Reference
ここにコピペ。
![](https://static.igem.org/mediawiki/2015/9/97/Tokyo_Tech_textarea_bottom.png)