Difference between revisions of "Team:San Andres/Parts"

 
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<html xmlns="http://www.w3.org/1999/xhtml">
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<html>
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<head>
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   <title>&lt;endnote&gt;&lt;head&gt;
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&lt;title&gt;&lt;endnote&gt;&lt;head&gt;
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&lt;title&gt;</title>
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</head>
 
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<body>
 
<body>
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    .gwd-a-z37b {
−
}
+
      color: rgb(0, 0, 0);
−
.gwd-td-asn4 {
+
    }
−
border: 1px solid black;
+
    .gwd-div-2ed9 {
−
}
+
      position: absolute;
−
.gwd-a-chbv {
+
      width: 50px;
−
color: rgb(0, 0, 0);
+
      height: 31px;
−
}
+
      left: 713px;
−
.gwd-td-8c55 {
+
      top: 92px;
−
border: 1px solid black;
+
      font-family: 'Times New Roman';
−
}
+
      text-align: left;
−
.gwd-a-rf1i {
+
      color: rgb(0, 0, 0);
−
color: rgb(0, 0, 0);
+
      background-image: none;
−
}
+
    }
−
.gwd-td-jce5 {
+
    .gwd-td-1it6 {
−
border: 1px solid black;
+
      border: 2px solid black;
−
}
+
    }
−
.gwd-a-bm82 {
+
    .gwd-a-cfz2 {
−
color: rgb(0, 0, 0);
+
      color: rgb(0, 0, 0);
−
}
+
    }
−
.gwd-td-7vtt {
+
    .gwd-td-gm83 {
−
border: 1px solid black;
+
      border: 2px solid black;
−
}
+
    }
−
.gwd-a-ffa8 {
+
    .gwd-a-d494 {
−
color: rgb(0, 0, 0);
+
      color: rgb(0, 0, 0);
−
}
+
    }
−
.gwd-td-aqks {
+
    .gwd-td-werd {
−
border: 1px solid black;
+
      border: 2px solid black;
−
}
+
    }
−
.gwd-a-1thu {
+
    .gwd-a-feiv {
−
color: rgb(0, 0, 0);
+
      color: rgb(0, 0, 0);
−
background-image: none;
+
    }
−
background-color: rgb(255, 255, 255);
+
    .gwd-td-pvfp {
−
}
+
      border: 2px solid black;
−
.gwd-td-dgpg {
+
    }
−
border: 1px solid black;
+
    .gwd-a-laum {
−
}
+
      color: rgb(0, 0, 0);
−
.gwd-a-cpqy {
+
    }
−
color: rgb(0, 0, 0);
+
    .gwd-td-yx5y {
−
}
+
      border: 2px solid black;
−
.gwd-td-788u {
+
    }
−
border: 1px solid black;
+
    .gwd-a-iekk {
−
}
+
      color: rgb(0, 0, 0);
−
.gwd-a-z37b {
+
    }
−
color: rgb(0, 0, 0);
+
    .gwd-td-844w {
−
}
+
      border: 2px solid black;
−
.gwd-div-2ed9 {
+
    }
−
position: absolute;
+
    .gwd-a-njb2 {
−
width: 50px;
+
      color: rgb(0, 0, 0);
−
height: 31px;
+
    }
−
left: 713px;
+
    .gwd-td-g48r {
−
top: 92px;
+
      border: 2px solid black;
−
font-family: 'Times New Roman';
+
    }
−
text-align: left;
+
    .gwd-a-r862 {
−
color: rgb(0, 0, 0);
+
      color: rgb(0, 0, 0);
−
background-image: none;
+
    }
−
}
+
    .gwd-td-sdu6 {
−
.gwd-td-1it6 {
+
      border: 2px solid black;
−
border: 2px solid black;
+
    }
−
}
+
    .gwd-a-tjrm {
−
.gwd-a-cfz2 {
+
      color: rgb(0, 0, 0);
−
color: rgb(0, 0, 0);
+
    }
−
}
+
    .gwd-td-4dr4 {
−
.gwd-td-gm83 {
+
      border: 2px solid black;
−
border: 2px solid black;
+
    }
−
}
+
    .gwd-a-yt4x {
−
.gwd-a-d494 {
+
      color: rgb(0, 0, 0);
−
color: rgb(0, 0, 0);
+
    }
−
}
+
    .gwd-div-oiqr {
−
.gwd-td-werd {
+
      background-image: none;
−
border: 2px solid black;
+
      background-color: rgb(255, 255, 255);
−
}
+
    }
−
.gwd-a-feiv {
+
    .gwd-img-voqe {
−
color: rgb(0, 0, 0);
+
      background-image: none;
−
}
+
    }
−
.gwd-td-pvfp {
+
    .gwd-img-kwqf {
−
border: 2px solid black;
+
      position: absolute;
−
}
+
      width: 100px;
−
.gwd-a-laum {
+
      height: 100px;
−
color: rgb(0, 0, 0);
+
      left: 856px;
−
}
+
      top: 13px;
−
.gwd-td-yx5y {
+
    }
−
border: 2px solid black;
+
    .editable {
−
}
+
      height: 67px;
−
.gwd-a-iekk {
+
      width: 913px;
−
color: rgb(0, 0, 0);
+
    }
−
}
+
    .gwd-tbody-gyzl {
−
.gwd-td-844w {
+
      border-color: rgb(128, 128, 128);
−
border: 2px solid black;
+
      height: 67px;
−
}
+
      width: 913px;
−
.gwd-a-njb2 {
+
      border-spacing: 2px;
−
color: rgb(0, 0, 0);
+
    }
−
}
+
    .gwd-tr-djv5 {
−
.gwd-td-g48r {
+
      border-color: rgb(128, 128, 128);
−
border: 2px solid black;
+
      height: 1px;
−
}
+
      vertical-align: middle;
−
.gwd-a-r862 {
+
      width: 913px;
−
color: rgb(0, 0, 0);
+
      border-spacing: 2px;
−
}
+
    }
−
.gwd-td-sdu6 {
+
    .gwd-tr-bjrl {
−
border: 2px solid black;
+
      border-color: rgb(128, 128, 128);
−
}
+
      height: 60px;
−
.gwd-a-tjrm {
+
      vertical-align: middle;
−
color: rgb(0, 0, 0);
+
      width: 913px;
−
}
+
      border-spacing: 2px;
−
.gwd-td-4dr4 {
+
    }
−
border: 2px solid black;
+
    .gwd-td-fbho {
−
}
+
      height: 54px;
−
.gwd-a-yt4x {
+
      padding: 1px;
−
color: rgb(0, 0, 0);
+
      vertical-align: middle;
−
}
+
      width: 59px;
−
.gwd-div-oiqr {
+
      border-spacing: 2px;
−
background-image: none;
+
      background-color: rgb(255, 166, 0);
−
background-color: rgb(255, 255, 255);
+
    }
−
}
+
    .gwd-a-qjw8 {
−
.gwd-img-voqe {
+
      text-decoration: underline;
−
background-image: none;
+
      border-spacing: 2px;
−
}
+
      -webkit-text-decorations-in-effect: underline;
−
.gwd-img-kwqf {
+
      width: auto;
−
position: absolute;
+
    }
−
width: 100px;
+
    .gwd-td-ttm2 {
−
height: 100px;
+
      height: 54px;
−
left: 856px;
+
      padding: 1px;
−
top: 13px;
+
      vertical-align: middle;
−
}
+
      width: 55px;
−
.editable {
+
      border-spacing: 2px;
−
height: 67px;
+
      background-color: rgb(255, 166, 0);
−
width: 913px;
+
    }
−
}
+
    .gwd-a-l1sa {
−
.gwd-tbody-gyzl {
+
      text-decoration: underline;
−
border-color: rgb(128, 128, 128);
+
      border-spacing: 2px;
−
height: 67px;
+
      -webkit-text-decorations-in-effect: underline;
−
width: 913px;
+
      width: auto;
−
border-spacing: 2px;
+
    }
−
}
+
    .gwd-td-kp4t {
−
.gwd-tr-djv5 {
+
      height: 54px;
−
border-color: rgb(128, 128, 128);
+
      padding: 1px;
−
height: 1px;
+
      vertical-align: middle;
−
vertical-align: middle;
+
      width: 95px;
−
width: 913px;
+
      border-spacing: 2px;
−
border-spacing: 2px;
+
      background-color: rgb(255, 166, 0);
−
}
+
    }
−
.gwd-tr-bjrl {
+
    .gwd-a-1gr5 {
−
border-color: rgb(128, 128, 128);
+
      text-decoration: underline;
−
height: 60px;
+
      border-spacing: 2px;
−
vertical-align: middle;
+
      -webkit-text-decorations-in-effect: underline;
−
width: 913px;
+
      width: auto;
−
border-spacing: 2px;
+
    }
−
}
+
    .gwd-td-jvud {
−
.gwd-td-fbho {
+
      height: 54px;
−
height: 54px;
+
      padding: 1px;
−
padding: 1px;
+
      vertical-align: middle;
−
vertical-align: middle;
+
      width: 68px;
−
width: 59px;
+
      border-spacing: 2px;
−
border-spacing: 2px;
+
      background-color: rgb(255, 166, 0);
−
background-color: rgb(255, 166, 0);
+
    }
−
}
+
    .gwd-a-fbim {
−
.gwd-a-qjw8 {
+
      text-decoration: underline;
−
text-decoration: underline;
+
      border-spacing: 2px;
−
border-spacing: 2px;
+
      -webkit-text-decorations-in-effect: underline;
−
-webkit-text-decorations-in-effect: underline;
+
      width: auto;
−
width: auto;
+
    }
−
}
+
    .gwd-td-t42m {
−
.gwd-td-ttm2 {
+
      height: 54px;
−
height: 54px;
+
      padding: 1px;
−
padding: 1px;
+
      vertical-align: middle;
−
vertical-align: middle;
+
      width: 48px;
−
width: 55px;
+
      border-spacing: 2px;
−
border-spacing: 2px;
+
      background-color: rgb(255, 166, 0);
−
background-color: rgb(255, 166, 0);
+
    }
−
}
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    .gwd-a-1qke {
−
.gwd-a-l1sa {
+
      text-decoration: underline;
−
text-decoration: underline;
+
      border-spacing: 2px;
−
border-spacing: 2px;
+
      -webkit-text-decorations-in-effect: underline;
−
-webkit-text-decorations-in-effect: underline;
+
      width: auto;
−
width: auto;
+
    }
−
}
+
    .gwd-td-l6h1 {
−
.gwd-td-kp4t {
+
      padding: 1px;
−
height: 54px;
+
      vertical-align: middle;
−
padding: 1px;
+
      width: 94px;
−
vertical-align: middle;
+
      border-spacing: 2px;
−
width: 95px;
+
    }
−
border-spacing: 2px;
+
    .gwd-a-4l4u {
−
background-color: rgb(255, 166, 0);
+
      text-decoration: underline;
−
}
+
      border-spacing: 2px;
−
.gwd-a-1gr5 {
+
      -webkit-text-decorations-in-effect: underline;
−
text-decoration: underline;
+
      width: auto;
−
border-spacing: 2px;
+
    }
−
-webkit-text-decorations-in-effect: underline;
+
    .gwd-td-rdpo {
−
width: auto;
+
      height: 54px;
−
}
+
      padding: 1px;
−
.gwd-td-jvud {
+
      vertical-align: middle;
−
height: 54px;
+
      width: 71px;
−
padding: 1px;
+
      border-spacing: 2px;
−
vertical-align: middle;
+
      background-color: rgb(255, 166, 0);
−
width: 68px;
+
    }
−
border-spacing: 2px;
+
    .gwd-a-p1o8 {
−
background-color: rgb(255, 166, 0);
+
      text-decoration: underline;
−
}
+
      border-spacing: 2px;
−
.gwd-a-fbim {
+
      -webkit-text-decorations-in-effect: underline;
−
text-decoration: underline;
+
      width: auto;
−
border-spacing: 2px;
+
    }
−
-webkit-text-decorations-in-effect: underline;
+
    .gwd-td-hpsl {
−
width: auto;
+
      height: 54px;
−
}
+
      padding: 1px;
−
.gwd-td-t42m {
+
      vertical-align: middle;
−
height: 54px;
+
      width: 87px;
−
padding: 1px;
+
      border-spacing: 2px;
−
vertical-align: middle;
+
      background-color: rgb(255, 166, 0);
−
width: 48px;
+
    }
−
border-spacing: 2px;
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    .gwd-a-i4cb {
−
background-color: rgb(255, 166, 0);
+
      text-decoration: underline;
−
}
+
      border-spacing: 2px;
−
.gwd-a-1qke {
+
      -webkit-text-decorations-in-effect: underline;
−
text-decoration: underline;
+
      width: auto;
−
border-spacing: 2px;
+
    }
−
-webkit-text-decorations-in-effect: underline;
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    .gwd-td-1973 {
−
width: auto;
+
      height: 54px;
−
}
+
      padding: 1px;
−
.gwd-td-l6h1 {
+
      vertical-align: middle;
−
padding: 1px;
+
      width: 108px;
−
vertical-align: middle;
+
      border-spacing: 2px;
−
width: 94px;
+
      background-color: rgb(255, 166, 0);
−
border-spacing: 2px;
+
    }
−
}
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    .gwd-a-y74o {
−
.gwd-a-4l4u {
+
      text-decoration: underline;
−
text-decoration: underline;
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      border-spacing: 2px;
−
border-spacing: 2px;
+
      -webkit-text-decorations-in-effect: underline;
−
-webkit-text-decorations-in-effect: underline;
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      width: auto;
−
width: auto;
+
    }
−
}
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    .gwd-td-s8h0 {
−
.gwd-td-rdpo {
+
      height: 54px;
−
height: 54px;
+
      padding: 1px;
−
padding: 1px;
+
      vertical-align: middle;
−
vertical-align: middle;
+
      width: 142px;
−
width: 71px;
+
      border-spacing: 2px;
−
border-spacing: 2px;
+
      background-color: rgb(255, 166, 0);
−
background-color: rgb(255, 166, 0);
+
    }
−
}
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    .gwd-a-zg9r {
−
.gwd-a-p1o8 {
+
      text-decoration: underline;
−
text-decoration: underline;
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      border-spacing: 2px;
−
border-spacing: 2px;
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      -webkit-text-decorations-in-effect: underline;
−
-webkit-text-decorations-in-effect: underline;
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      width: auto;
−
width: auto;
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    }
−
}
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    .gwd-td-8a5b {
−
.gwd-td-hpsl {
+
      height: 58px;
−
height: 54px;
+
      padding: 1px;
−
padding: 1px;
+
      vertical-align: middle;
−
vertical-align: middle;
+
      border-spacing: 2px;
−
width: 87px;
+
      width: 0px;
−
border-spacing: 2px;
+
    }
−
background-color: rgb(255, 166, 0);
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    .gwd-br-exed {
−
}
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      border-spacing: 2px;
−
.gwd-a-i4cb {
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      width: auto;
−
text-decoration: underline;
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    }
−
border-spacing: 2px;
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    .gwd-table-vk9d {
−
-webkit-text-decorations-in-effect: underline;
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      -webkit-transform-style: preserve-3d;
−
width: auto;
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      -webkit-transform: translate3d(0px, -4px, 0px);
−
}
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    }
−
.gwd-td-1973 {
+
    body .gwd-gen-goi7gwdanimation {
−
height: 54px;
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      -webkit-animation: gwd-gen-goi7gwdanimation_gwd-keyframes 0.5s linear 0s 1 normal forwards;
−
padding: 1px;
+
    }
−
vertical-align: middle;
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    @-webkit-keyframes gwd-gen-goi7gwdanimation_gwd-keyframes {
−
width: 108px;
+
      0% {
−
border-spacing: 2px;
+
        -webkit-transform: translate3d(0px, -4px, 0px);
−
background-color: rgb(255, 166, 0);
+
        -webkit-animation-timing-function: linear;
−
}
+
      }
−
.gwd-a-y74o {
+
      100% {
−
text-decoration: underline;
+
        -webkit-transform: translate3d(0px, -1px, 0px);
−
border-spacing: 2px;
+
        -webkit-animation-timing-function: linear;
−
-webkit-text-decorations-in-effect: underline;
+
      }
−
width: auto;
+
    }
−
}
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    .gwd-a-3xpx {
−
.gwd-td-s8h0 {
+
      -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0.05, 0, 0, 1);
−
height: 54px;
+
      -webkit-transform-style: preserve-3d;
−
padding: 1px;
+
    }
−
vertical-align: middle;
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    .gwd-img-n53q {
−
width: 142px;
+
      position: absolute;
−
border-spacing: 2px;
+
      width: 123px;
−
background-color: rgb(255, 166, 0);
+
      left: -161.35px;
−
}
+
      top: 218px;
−
.gwd-a-zg9r {
+
    }
−
text-decoration: underline;
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    .gwd-img-kryd {
−
border-spacing: 2px;
+
      position: absolute;
−
-webkit-text-decorations-in-effect: underline;
+
      left: 1009.61157635431px;
−
width: auto;
+
      top: 221.560837438423px;
−
}
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      width: 250px;
−
.gwd-td-8a5b {
+
    }
−
height: 58px;
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    .gwd-img-ia0j {
−
padding: 1px;
+
      position: absolute;
−
vertical-align: middle;
+
      width: 135px;
−
border-spacing: 2px;
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      left: -166.263300492612px;
−
width: 0px;
+
      top: 394.466009852218px;
−
}
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    }
−
.gwd-br-exed {
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    .gwd-a-36ww {
−
border-spacing: 2px;
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      -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, -16.1, 0.05, 0, 1);
−
width: auto;
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      -webkit-transform-style: preserve-3d;
−
}
+
    }
−
.gwd-table-vk9d {
+
    .gwd-body-kwce {
−
-webkit-transform-style: preserve-3d;
+
      width: 100%;
−
-webkit-transform: translate3d(0px, -4px, 0px);
+
    }
−
}
+
    .gwd-img-5ywk {
−
body .gwd-gen-goi7gwdanimation {
+
      position: absolute;
−
-webkit-animation: gwd-gen-goi7gwdanimation_gwd-keyframes 0.5s linear 0s 1 normal forwards;
+
      left: 990.561576354683px;
−
}
+
      top: 349.384236453212px;
−
@-webkit-keyframes gwd-gen-goi7gwdanimation_gwd-keyframes {
+
      width: 250px;
−
0% {
+
    }
−
-webkit-transform: translate3d(0px, -4px, 0px);
+
    .gwd-img-zpsn {
−
-webkit-animation-timing-function: linear;
+
      position: absolute;
−
}
+
      width: 150px;
−
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Latest revision as of 13:02, 15 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pSB1C3
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:Parts.jpg
File:Circuito.png