Difference between revisions of "Team:San Andres/Parts"

 
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<head data-gwd-animation-mode="quickMode">
 
<head data-gwd-animation-mode="quickMode">
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  <title>&lt;endnote&gt;&lt;head&gt; &lt;title&gt;&lt;endnote&gt;&lt;head&gt; &lt;meta&gt; &lt;title&gt;
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<body>
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   <title>wiki</title>
 
   <title>wiki</title>
 
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   <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 
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   <title></title>
 
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   <title>wiki 2</title>
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   <!--navigation menu -->
 
   <!--navigation menu -->
−
   <table class="gwd-table-nb4m editable" width="100%">
+
  <br>
 +
   <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-15ao">
 
     <tbody>
 
     <tbody>
 
       <tr heigth="15px">
 
       <tr heigth="15px">
 
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       <tr heigth="75px">
 
       <tr heigth="75px">
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         <td style="border: 2px solid black;" align="center" bgcolor="FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#FFBA00'">
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         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 
           <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>  
 
           <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>  
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
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         <td style="border: 3px solid black;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team </a>  
+
           <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
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−
           <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project </a>
+
           <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project</a>
 
         </td>
 
         </td>
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+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);"> Statistics</a>
+
           <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://2015.igem.org/Team:San_Andres/Experiments" style="color: rgb(0, 0, 0);"> Enzymes</a>
+
           <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Modeling</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"><a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
−
          <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
+
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
      </tr>
−
           <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Metodology</a>
+
    </tbody>
 +
  </table>
 +
  <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-10nh">
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 +
           <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);">Notebook</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results </a>
+
           <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#FF8600" height="45" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Future Projections </a>
+
           <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
 
         </td>
 
         </td>
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+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://igem.org/Team_List?year=2015">
+
           <a href="https://2015.igem.org/Team:San_Andres/Attributions" style="color: rgb(0, 0, 0);"> Attributions</a>
−
            <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55">
+
        </td>
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          </a>
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        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
 
         </td>
 
         </td>
 
       </tr>
 
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<div class="gwd-div-2ed9"></div>
 
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<div class="gwd-div-7xzo gwd-a-1thu">
 
<div class="gwd-div-7xzo gwd-a-1thu">
−
<h1>Parts&nbsp;</h1>
+
<h1 style="font-family: Algerian;"><big>Parts&nbsp;</big></h1>
−
<big>Throughout the project we started to learn the fundamental
+
<div style="text-align: justify; font-family: Cambria;"><big>Throughout
 +
the project we started to learn the fundamental
 
principles of synthetic biology to get to work on our plasmid with
 
principles of synthetic biology to get to work on our plasmid with
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
this we going to insert in an e. coli the parts (Biobricks) needed to
 
this we going to insert in an e. coli the parts (Biobricks) needed to
−
make our future bacteria can degrade gluten. The parts are:<br>
+
make our future bacteria can degrade gluten. The parts are:</big><br />
−
</big>
+
</div>
−
<ul>
+
<ul style="font-family: Cambria;">
−
   <li><big><span
+
   <li style="text-align: justify;"><big><strong
−
style="font-family: 'Arial','sans-serif';"><strong
+
  style="font-weight: bold;">Promoter
−
  style="font-weight: bold;">Promoter (BBa_J23119</strong><span
+
    <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a
−
  style="font-weight: bold;">)</span>: </span>Constitutive
+
href="http://parts.igem.org/Part:BBa_J23119"><span
 +
  style="font-weight: bold;">)</span></a>:
 +
Constitutive
 
promoter (which works permanently) that is give in the relative
 
promoter (which works permanently) that is give in the relative
−
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big></li>
+
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
−
   <li><big><strong style="font-weight: bold;">RBS
+
  </li>
−
(BBa_K1084103)</strong>: Synthetic RBS with uplifting sequence.</big></li>
+
   <li style="text-align: justify;"><big><strong
−
   <li><big><strong><span
+
style="font-weight: bold;">RBS</strong><strong
−
  style="font-family: 'Arial','sans-serif';">Vector:<span
+
style="font-weight: bold;">
−
  style="font-weight: normal;"> pET29B+</span></span></strong></big></li>
+
    <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
−
   <li><big><strong><span
+
Synthetic RBS with uplifting sequence</big>.</li>
−
style="font-family: 'Arial','sans-serif';">Coding Region:
+
   <li style="text-align: justify;"><big><strong>Vector:<span
−
KumaMax (BBa_K590087):</span></strong> It degrades gluten,
+
  style="font-weight: normal;"> <a
 +
  href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
 +
  </li>
 +
   <li style="text-align: justify;"><big><strong>Coding
 +
Region:
 +
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</strong>
 +
It
 +
degrades gluten,
 
celiac disease leading cause. Enzyme generated by rational mutation for
 
celiac disease leading cause. Enzyme generated by rational mutation for
−
the active site of it.</big></li>
+
the active site of it. It was created by the team IGEM <a
−
  <li><big><strong><span
+
href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
−
style="font-family: 'Arial','sans-serif';">Reporter: RFP
+
2011.</a></big>
−
(BBa_J04450):</span></strong><span
+
  </li>
−
style="font-family: Arial,sans-serif;"> Red f</span>luorescence
+
  <li style="text-align: justify;"><big><strong>Reporter:
−
protein.</big></li>
+
RFP
−
   <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
+
    <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</strong>
−
(BBa_B0015):</strong> </span>Dual terminator consisting of
+
Red fluorescence
 +
protein.</big>
 +
  </li>
 +
   <li style="text-align: justify;"><big><strong>Terminator
 +
    <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
 +
Dual terminator consisting of
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
transcription</big>.</li>
 
transcription</big>.</li>
 
</ul>
 
</ul>
−
<div style="text-align: center;"><img alt="File:1.png"
+
<div style="text-align: center; font-family: Cambria;">
−
  src="https://static.igem.org/mediawiki/2015/4/4a/1.png" height="68"
+
<img alt="File:Parts.jpg"
−
width="312"><br>
+
  src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg"
−
<img alt="File:Circuito.jpg"
+
height="59" width="288" /><br />
−
  src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg"
+
<img
−
height="309" width="800"><br>
+
  src="https://static.igem.org/mediawiki/2015/thumb/3/34/Circuito.png/800px-Circuito.png"
−
<div style="text-align: left;"><big>This is a
+
  alt="File:Circuito.png" />&nbsp;<br />
−
graphic model of as it has be our plasmid where we can visualize the
+
−
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
+
−
joined by means of prefixes and suffixes that indicate the locations of
+
−
court.</big><br>
+
−
<br>
+
−
<div style="text-align: center;"><img
+
−
  alt="File:Plasmido.jpg"
+
−
src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg"
+
−
height="600" width="664"></div>
+
−
</div>
+
−
</div>
+
−
<big><br>
+
−
</big>
+
−
<div style="text-align: center;"><br>
+
−
<br>
+
−
</div>
+
 
</div>
 
</div>
 +
<big style="font-family: Cambria;">
 +
</big></div>
 +
<br style="font-family: Cambria;" />
 +
<a style="font-family: Cambria;"
 +
href="https://2015.igem.org/Main_Page"
 +
class="gwd-a-3xpx gwd-a-36ww">
 +
<img
 +
style="border: 0px solid ; width: 150px; height: 150px; left: 989px; top: 49px;"
 +
alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
 +
class="gwd-img-kwqf" /></a><a
 +
style="font-family: Cambria;"
 +
href="https://igem.org/Team_List?year=2015"
 +
class="gwd-a-3xpx gwd-a-d01x"><img
 +
src="https://static.igem.org/mediawiki/2015/thumb/8/85/Logo_Definitvo.png/654px-Logo_Definitvo.png"
 +
class="gwd-img-zpsn" /></a><br
 +
style="font-family: Cambria;" />
 +
<br style="font-family: Cambria;" />
 +
<br />
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 13:02, 15 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pSB1C3
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:Parts.jpg
File:Circuito.png