Difference between revisions of "Team:San Andres/Parts"
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<html> | <html> | ||
− | + | ||
− | + | <head data-gwd-animation-mode="quickMode"> | |
− | + | <title><endnote><head> <title><endnote><head> <meta> <title> | |
− | <title></title> | + | </title> |
</head> | </head> | ||
+ | |||
<body> | <body> | ||
− | <meta content="text/html; charset=ISO-8859-1" | + | <title>wiki</title> |
− | + | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> | |
− | <title></title> | + | <meta name="generator" content="Google Web Designer 1.3.2.0521"> |
− | <meta content="text/html; charset=ISO-8859-1" | + | <style type="text/css"> |
− | + | html, body { | |
− | <title></title> | + | width: 100%; |
− | <meta content="text/html; charset=ISO-8859-1" | + | height: 100%; |
− | + | margin: 0px; | |
− | <title></title> | + | } |
− | <title> | + | body { |
− | <meta http-equiv="Content-Type" | + | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1); |
− | + | -webkit-transform-style: preserve-3d; | |
− | <meta name="generator" | + | background-image: url(https://static.igem.org/mediawiki/2015/8/8a/BBD7.jpg); |
− | + | background-color: rgb(198, 169, 117); | |
− | <style type="text/css"> | + | } |
− | html, body { | + | </style> |
− | width: 100%; | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | height: 100%; | + | <title></title> |
− | margin: 0px; | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | } | + | <title></title> |
− | body { | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1); | + | <title></title> |
− | -webkit-transform-style: preserve-3d; | + | <title>wiki</title> |
− | background-color: | + | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> |
− | + | <meta name="generator" content="Google Web Designer 1.3.2.0521"> | |
− | } | + | <style type="text/css"> |
− | </style> | + | html, body { |
− | <meta http-equiv="content-type" | + | width: 100%; |
− | + | height: 100%; | |
− | <title></title> | + | margin: 0px; |
− | <meta http-equiv="content-type" | + | } |
− | + | body { | |
− | <title></title> | + | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1); |
− | <meta http-equiv="content-type" | + | -webkit-transform-style: preserve-3d; |
− | + | background-color: transparent; | |
− | <title></title> | + | } |
− | <title>wiki | + | </style> |
− | <meta http-equiv="Content-Type" | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | + | <title></title> | |
− | <meta name="generator" | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | + | <title></title> | |
− | <img style="width: 962px; height: | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | + | <title></title> | |
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− | <style type="text/css" class=""> | + | <title></title> |
− | html, body { | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | width: 100%; | + | <title></title> |
− | height: 10%; | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | margin: 0px; | + | <title></title> |
− | } | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | body { | + | <title></title> |
− | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1); | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | -webkit-transform-style: preserve-3d; | + | <title></title> |
− | background-color: transparent; | + | <title>exp</title> |
− | } | + | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> |
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− | font-size: 24px; | + | <style type="text/css"> |
− | -webkit-transform: translate3d(0px, -2px, 0px); | + | html, body { |
− | -webkit-transform-style: preserve-3d; | + | width: 100%; |
− | background-image: none; | + | height: 100%; |
− | background-color: rgb(255, 255, 255); | + | margin: 0px; |
− | } | + | } |
− | .gwd-td-b6db { | + | body { |
− | border: 1px solid black; | + | -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1); |
− | } | + | -webkit-transform-style: preserve-3d; |
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− | border: 1px solid black; | + | <title></title> |
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− | color: rgb(0, 0, 0); | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | } | + | <title></title> |
− | .gwd-td-jbkf { | + | <title>wiki</title> |
− | border: 1px solid black; | + | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> |
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− | border: 1px solid black; | + | <title></title> |
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− | color: rgb(0, 0, 0); | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
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− | border: 1px solid black; | + | <title></title> |
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− | color: rgb(0, 0, 0); | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
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− | border: 1px solid black; | + | <title></title> |
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− | .gwd-a-mwsi { | + | <title></title> |
− | color: rgb(0, 0, 0); | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
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− | .gwd-td-zyck { | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | border: 1px solid black; | + | <title></title> |
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− | .gwd-a-j8kh { | + | <title></title> |
− | color: rgb(0, 0, 0); | + | <title>wiki</title> |
− | } | + | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> |
− | .gwd-td-uz3t { | + | <meta name="generator" content="Google Web Designer 1.3.2.0521"> |
− | border: 1px solid black; | + | <style type="text/css"> |
− | } | + | html, body { |
− | .gwd-a-83hm { | + | width: 100%; |
− | color: rgb(0, 0, 0); | + | height: 100%; |
− | } | + | margin: 0px; |
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− | border: 1px solid black; | + | body { |
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− | .gwd-a-80zp { | + | -webkit-transform-style: preserve-3d; |
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− | } | + | </style> |
− | .gwd-td-asn4 { | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | border: 1px solid black; | + | <title></title> |
− | } | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | .gwd-a-chbv { | + | <title></title> |
− | color: rgb(0, 0, 0); | + | <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> |
− | } | + | <title></title> |
− | .gwd-td-8c55 { | + | <title>wiki 2</title> |
− | border: 1px solid black; | + | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> |
− | } | + | <meta name="generator" content="Google Web Designer 1.3.2.0521"> |
− | .gwd-a-rf1i { | + | <img style="width: 962px; height: 325px;" src="https://static.igem.org/mediawiki/2015/thumb/8/8b/Gluten-s-Job.jpeg/800px-Gluten-s-Job.jpeg" alt="File:Gluten-s-Job.jpeg"> |
− | color: rgb(0, 0, 0); | + | <style type="text/css" class=""> |
− | } | + | html, body { |
− | .gwd-td-jce5 { | + | width: 100%; |
− | border: 1px solid black; | + | height: 10%; |
− | } | + | margin: 0px; |
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− | color: rgb(0, 0, 0); | + | body { |
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− | .gwd-td-7vtt { | + | -webkit-transform-style: preserve-3d; |
− | border: 1px solid black; | + | background-color: transparent; |
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− | border: 1px solid black; | + | background-image: none; |
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− | color: rgb(0, 0, 0); | + | .gwd-td-b6db { |
− | background-image: none; | + | border: 1px solid black; |
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− | font-family: 'Times New Roman'; | + | border: 1px solid black; |
− | text-align: left; | + | } |
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− | .gwd-img-voqe { | + | color: rgb(0, 0, 0); |
− | background-image: none; | + | } |
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− | </style><!--navigation menu --> | + | border: 1px solid black; |
− | <table class="gwd-table-nb4m editable | + | } |
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− | + | .gwd-td-sdu6 { | |
− | </table> | + | border: 2px solid black; |
+ | } | ||
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+ | } | ||
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+ | border: 2px solid black; | ||
+ | } | ||
+ | .gwd-a-yt4x { | ||
+ | color: rgb(0, 0, 0); | ||
+ | } | ||
+ | .gwd-div-oiqr { | ||
+ | background-image: none; | ||
+ | background-color: rgb(255, 255, 255); | ||
+ | } | ||
+ | .gwd-img-voqe { | ||
+ | background-image: none; | ||
+ | } | ||
+ | .gwd-img-kwqf { | ||
+ | position: absolute; | ||
+ | width: 100px; | ||
+ | height: 100px; | ||
+ | left: 856px; | ||
+ | top: 13px; | ||
+ | } | ||
+ | .editable { | ||
+ | height: 67px; | ||
+ | width: 913px; | ||
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+ | .gwd-tbody-gyzl { | ||
+ | border-color: rgb(128, 128, 128); | ||
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+ | height: 54px; | ||
+ | padding: 1px; | ||
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+ | <!--navigation menu --> | ||
+ | <br> | ||
+ | <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-15ao"> | ||
+ | <tbody> | ||
+ | <tr heigth="15px"> | ||
+ | </tr> | ||
+ | <tr heigth="75px"> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid black;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Modeling</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"><a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-10nh"> | ||
+ | <tbody> | ||
+ | <tr heigth="15px"> | ||
+ | </tr> | ||
+ | <tr heigth="75px"> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);">Notebook</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Attributions" style="color: rgb(0, 0, 0);"> Attributions</a> | ||
+ | </td> | ||
+ | <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
<div class="gwd-div-2ed9"></div> | <div class="gwd-div-2ed9"></div> | ||
<div class="gwd-div-7xzo gwd-a-1thu"> | <div class="gwd-div-7xzo gwd-a-1thu"> | ||
− | <h1>Parts </h1> | + | <h1 style="font-family: Algerian;"><big>Parts </big></h1> |
− | <big>Throughout the project we started to learn the fundamental | + | <div style="text-align: justify; font-family: Cambria;"><big>Throughout |
+ | the project we started to learn the fundamental | ||
principles of synthetic biology to get to work on our plasmid with | principles of synthetic biology to get to work on our plasmid with | ||
which we want to see gluten degradation via the enzyme Kumamax. For | which we want to see gluten degradation via the enzyme Kumamax. For | ||
this we going to insert in an e. coli the parts (Biobricks) needed to | this we going to insert in an e. coli the parts (Biobricks) needed to | ||
− | make our future bacteria can degrade gluten. The parts are:<br> | + | make our future bacteria can degrade gluten. The parts are:</big><br /> |
− | </ | + | </div> |
− | <ul> | + | <ul style="font-family: Cambria;"> |
− | <li | + | <li style="text-align: justify;"><big><strong |
− | + | style="font-weight: bold;">Promoter | |
− | style="font-weight: bold;"><a | + | <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a |
− | + | href="http://parts.igem.org/Part:BBa_J23119"><span | |
− | + | style="font-weight: bold;">)</span></a>: | |
− | + | Constitutive | |
promoter (which works permanently) that is give in the relative | promoter (which works permanently) that is give in the relative | ||
− | fluorescence of these plasmids in the TG1 strain grown in LB medium.</big></li> | + | fluorescence of these plasmids in the TG1 strain grown in LB medium.</big> |
− | <li><big><strong style="font-weight: bold;">< | + | </li> |
− | href="http://parts.igem.org/Part:BBa_K1084103"> | + | <li style="text-align: justify;"><big><strong |
− | + | style="font-weight: bold;">RBS</strong><strong | |
− | <li><big><strong><span | + | style="font-weight: bold;"> |
− | style="font- | + | <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>: |
− | href="http://parts.igem.org/Part:pSB1C3"> | + | Synthetic RBS with uplifting sequence</big>.</li> |
− | + | <li style="text-align: justify;"><big><strong>Vector:<span | |
− | <li><big><strong> | + | style="font-weight: normal;"> <a |
− | + | href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big> | |
− | + | </li> | |
− | + | <li style="text-align: justify;"><big><strong>Coding | |
+ | Region: | ||
+ | KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</strong> | ||
+ | It | ||
degrades gluten, | degrades gluten, | ||
celiac disease leading cause. Enzyme generated by rational mutation for | celiac disease leading cause. Enzyme generated by rational mutation for | ||
− | the active site of it.</ | + | the active site of it. It was created by the team IGEM <a |
− | + | href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington | |
− | + | 2011.</a></big> | |
− | + | </li> | |
− | (BBa_J04450) | + | <li style="text-align: justify;"><big><strong>Reporter: |
− | + | RFP | |
− | protein.</big></li> | + | <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</strong> |
− | <li | + | Red fluorescence |
− | + | protein.</big> | |
− | + | </li> | |
+ | <li style="text-align: justify;"><big><strong>Terminator | ||
+ | <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong> | ||
+ | Dual terminator consisting of | ||
the B0010 and B0012 parties. It serves to give greater efficiency in | the B0010 and B0012 parties. It serves to give greater efficiency in | ||
transcription</big>.</li> | transcription</big>.</li> | ||
</ul> | </ul> | ||
− | <div style="text-align: center;"><img alt="File: | + | <div style="text-align: center; font-family: Cambria;"> |
− | src="https://static.igem.org/mediawiki/2015/ | + | <img alt="File:Parts.jpg" |
− | + | src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg" | |
− | <img | + | height="59" width="288" /><br /> |
− | src="https://static.igem.org/mediawiki/2015/thumb/ | + | <img |
− | + | src="https://static.igem.org/mediawiki/2015/thumb/3/34/Circuito.png/800px-Circuito.png" | |
− | + | alt="File:Circuito.png" /> <br /> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | alt="File: | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
− | <br> | + | <big style="font-family: Cambria;"> |
+ | </big></div> | ||
+ | <br style="font-family: Cambria;" /> | ||
+ | <a style="font-family: Cambria;" | ||
+ | href="https://2015.igem.org/Main_Page" | ||
+ | class="gwd-a-3xpx gwd-a-36ww"> | ||
+ | <img | ||
+ | style="border: 0px solid ; width: 150px; height: 150px; left: 989px; top: 49px;" | ||
+ | alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" | ||
+ | class="gwd-img-kwqf" /></a><a | ||
+ | style="font-family: Cambria;" | ||
+ | href="https://igem.org/Team_List?year=2015" | ||
+ | class="gwd-a-3xpx gwd-a-d01x"><img | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/8/85/Logo_Definitvo.png/654px-Logo_Definitvo.png" | ||
+ | class="gwd-img-zpsn" /></a><br | ||
+ | style="font-family: Cambria;" /> | ||
+ | <br style="font-family: Cambria;" /> | ||
+ | <br /> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 13:02, 15 September 2015
Home | Team | Project | Parts | Modeling | Results |
Notebook | Human Practices | Future Projections | Attributions | Collaborations |
Parts
Throughout
the project we started to learn the fundamental
principles of synthetic biology to get to work on our plasmid with
which we want to see gluten degradation via the enzyme Kumamax. For
this we going to insert in an e. coli the parts (Biobricks) needed to
make our future bacteria can degrade gluten. The parts are:
- Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
- RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
- Vector: pSB1C3
- Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
- Reporter: RFP (BBa_J04450): Red fluorescence protein.
- Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.