Difference between revisions of "Team:San Andres/Parts"

 
(10 intermediate revisions by the same user not shown)
Line 2: Line 2:
  
 
<head data-gwd-animation-mode="quickMode">
 
<head data-gwd-animation-mode="quickMode">
 +
  <title>&lt;endnote&gt;&lt;head&gt; &lt;title&gt;&lt;endnote&gt;&lt;head&gt; &lt;meta&gt; &lt;title&gt;
 +
  </title>
 +
</head>
 +
 +
<body>
 +
  <title>wiki</title>
 +
  <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 +
  <meta name="generator" content="Google Web Designer 1.3.2.0521">
 +
  <style type="text/css">
 +
    html, body {
 +
      width: 100%;
 +
      height: 100%;
 +
      margin: 0px;
 +
    }
 +
    body {
 +
      -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 +
      -webkit-transform-style: preserve-3d;
 +
      background-image: url(https://static.igem.org/mediawiki/2015/8/8a/BBD7.jpg);
 +
      background-color: rgb(198, 169, 117);
 +
    }
 +
  </style>
 +
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 +
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 +
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 
   <title>wiki</title>
 
   <title>wiki</title>
 
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
Line 17: Line 44:
 
     }
 
     }
 
   </style>
 
   </style>
−
</head>
 
−
 
−
<body class="gwd-body-kwce">
 
 
   <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 
   <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 
   <title></title>
 
   <title></title>
Line 106: Line 130:
 
       -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 
       -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
−
      background-image: url(https://static.igem.org/mediawiki/2015/8/8a/BBD7.jpg);
 
−
      background-color: rgb(198, 169, 117);
 
 
     }
 
     }
 
   </style>
 
   </style>
Line 517: Line 539:
 
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0.05, 0, 0, 1);
 
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0.05, 0, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
−
    }
 
−
    .gwd-img-l2p5 {
 
−
      position: absolute;
 
−
      width: 135px;
 
−
      left: -160px;
 
−
      top: 26px;
 
 
     }
 
     }
 
     .gwd-img-n53q {
 
     .gwd-img-n53q {
Line 543: Line 559:
 
     }
 
     }
 
     .gwd-a-36ww {
 
     .gwd-a-36ww {
−
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
+
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, -16.1, 0.05, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
 
     }
 
     }
Line 554: Line 570:
 
       top: 349.384236453212px;
 
       top: 349.384236453212px;
 
       width: 250px;
 
       width: 250px;
 +
    }
 +
    .gwd-img-zpsn {
 +
      position: absolute;
 +
      width: 150px;
 +
      left: -165.73913043478px;
 +
      top: 49.3260869565235px;
 +
    }
 +
    .gwd-a-d01x {
 +
      -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 +
      -webkit-transform-style: preserve-3d;
 +
    }
 +
    .gwd-table-10nh {
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 +
    }
 +
    .gwd-table-15ao {
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 
     }
 
     }
 
   </style>
 
   </style>
 
   <!--navigation menu -->
 
   <!--navigation menu -->
 
   <br>
 
   <br>
−
   <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable">
+
   <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-15ao">
 
     <tbody>
 
     <tbody>
 
       <tr heigth="15px">
 
       <tr heigth="15px">
 
       </tr>
 
       </tr>
 
       <tr heigth="75px">
 
       <tr heigth="75px">
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#FFBA00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 
           <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>  
 
           <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>  
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid black;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 
           <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
 
           <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);"> Notebook</a>
+
           <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
          <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project </a>
+
−
        </td>
+
−
        <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
 
           <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
 
           <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Modeling</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"><a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
 
         </td>
 
         </td>
 
       </tr>
 
       </tr>
 
     </tbody>
 
     </tbody>
 
   </table>
 
   </table>
−
   <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable">
+
   <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-10nh">
 
     <tbody>
 
     <tbody>
 
       <tr heigth="15px">
 
       <tr heigth="15px">
 
       </tr>
 
       </tr>
 
       <tr heigth="75px">
 
       <tr heigth="75px">
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#FFBA00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
           <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);">Modeling</a>
+
           <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);">Notebook</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
−
          <a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
+
−
        </td>
+
−
        <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
 
           <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
 
           <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 
           <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
 
           <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
 
         </td>
 
         </td>
−
         <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
+
         <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Attributions" style="color: rgb(0, 0, 0);"> Attributions</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 
           <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
 
           <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
 
         </td>
 
         </td>
Line 606: Line 642:
 
<div class="gwd-div-2ed9"></div>
 
<div class="gwd-div-2ed9"></div>
 
<div class="gwd-div-7xzo gwd-a-1thu">
 
<div class="gwd-div-7xzo gwd-a-1thu">
−
<h1>Parts&nbsp;</h1>
+
<h1 style="font-family: Algerian;"><big>Parts&nbsp;</big></h1>
−
<big>Throughout the project we started to learn the fundamental
+
<div style="text-align: justify; font-family: Cambria;"><big>Throughout
 +
the project we started to learn the fundamental
 
principles of synthetic biology to get to work on our plasmid with
 
principles of synthetic biology to get to work on our plasmid with
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
this we going to insert in an e. coli the parts (Biobricks) needed to
 
this we going to insert in an e. coli the parts (Biobricks) needed to
−
make our future bacteria can degrade gluten. The parts are:<br>
+
make our future bacteria can degrade gluten. The parts are:</big><br />
−
</big>
+
</div>
−
<ul>
+
<ul style="font-family: Cambria;">
−
   <li><big><span
+
   <li style="text-align: justify;"><big><strong
−
style="font-family: 'Arial','sans-serif';"><strong
+
 
  style="font-weight: bold;">Promoter
 
  style="font-weight: bold;">Promoter
 
     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a
 
     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a
 
  href="http://parts.igem.org/Part:BBa_J23119"><span
 
  href="http://parts.igem.org/Part:BBa_J23119"><span
 
  style="font-weight: bold;">)</span></a>:
 
  style="font-weight: bold;">)</span></a>:
−
    </span>Constitutive
+
Constitutive
 
promoter (which works permanently) that is give in the relative
 
promoter (which works permanently) that is give in the relative
−
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big></li>
+
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
−
   <li><big><strong style="font-weight: bold;">RBS</strong><strong
+
  </li>
 +
   <li style="text-align: justify;"><big><strong
 +
style="font-weight: bold;">RBS</strong><strong
 
  style="font-weight: bold;">
 
  style="font-weight: bold;">
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
Synthetic RBS with uplifting sequence</big>.</li>
 
Synthetic RBS with uplifting sequence</big>.</li>
−
   <li><big><strong>Vector:<span
+
   <li style="text-align: justify;"><big><strong>Vector:<span
 
  style="font-weight: normal;"> <a
 
  style="font-weight: normal;"> <a
−
  href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big></li>
+
  href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
−
   <li><big><strong><span
+
  </li>
−
style="font-family: 'Arial','sans-serif';">Coding Region:
+
   <li style="text-align: justify;"><big><strong>Coding
−
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
+
Region:
 +
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</strong>
 
It
 
It
 
degrades gluten,
 
degrades gluten,
Line 638: Line 677:
 
the active site of it. It was created by the team IGEM <a
 
the active site of it. It was created by the team IGEM <a
 
  href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
 
  href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
−
2011.</a></big></li>
+
2011.</a></big>
−
   <li><big><strong><span
+
  </li>
−
style="font-family: 'Arial','sans-serif';">Reporter: RFP
+
   <li style="text-align: justify;"><big><strong>Reporter:
−
     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span
+
RFP
−
style="font-family: Arial,sans-serif;"> Red f</span>luorescence
+
     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</strong>
−
protein.</big></li>
+
Red fluorescence
−
   <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
+
protein.</big>
 +
  </li>
 +
   <li style="text-align: justify;"><big><strong>Terminator
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
−
    </span>Dual terminator consisting of
+
Dual terminator consisting of
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
transcription</big>.</li>
 
transcription</big>.</li>
 
</ul>
 
</ul>
−
<div style="text-align: center;"><img
+
<div style="text-align: center; font-family: Cambria;">
−
alt="File:Parts.jpg"
+
<img alt="File:Parts.jpg"
 
  src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg"
 
  src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg"
−
  height="59" width="288"><br>
+
  height="59" width="288" /><br />
−
<img alt="File:Circuito.jpg"
+
<img
−
  src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg"
+
  src="https://static.igem.org/mediawiki/2015/thumb/3/34/Circuito.png/800px-Circuito.png"
−
height="309" width="800"><br>
+
  alt="File:Circuito.png" />&nbsp;<br />
−
<div style="text-align: left;"><big>This is a
+
−
graphic model of as it has be our plasmid where we can visualize the
+
−
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
+
−
joined by means of prefixes and suffixes that indicate the locations of
+
−
court.</big><br>
+
−
<br>
+
−
<div style="text-align: center;"><img
+
−
  alt="File:Plasmido.jpg"
+
−
src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg"
+
−
height="600" width="664"></div>
+
 
</div>
 
</div>
−
</div>
+
<big style="font-family: Cambria;">
−
<big><br>
+
</big></div>
−
</big>
+
<br style="font-family: Cambria;" />
−
</div>
+
<a style="font-family: Cambria;"
−
<br>
+
href="https://2015.igem.org/Main_Page"
−
  <a href="https://2015.igem.org/Main_Page" class="gwd-a-3xpx gwd-a-36ww">
+
class="gwd-a-3xpx gwd-a-36ww">
−
    <img style="border: 0px solid; width: 150px; height: 150px; left: 1032.48768472907px; top: 56.128078817734px;" alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" class="gwd-img-kwqf">
+
<img
−
    <img src="https://static.igem.org/mediawiki/2015/3/3d/Unesco_2.png" class="gwd-img-5ywk">
+
style="border: 0px solid ; width: 150px; height: 150px; left: 989px; top: 49px;"
−
  </a>
+
alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
−
  <a href="https://igem.org/Team_List?year=2015" class="gwd-a-3xpx">
+
class="gwd-img-kwqf" /></a><a
−
    <img src="https://static.igem.org/mediawiki/2015/thumb/9/94/Logo43.png/454px-Logo43.png" class="gwd-img-l2p5">
+
style="font-family: Cambria;"
−
  </a>
+
href="https://igem.org/Team_List?year=2015"
−
  <br>
+
class="gwd-a-3xpx gwd-a-d01x"><img
−
  <br>
+
src="https://static.igem.org/mediawiki/2015/thumb/8/85/Logo_Definitvo.png/654px-Logo_Definitvo.png"
−
  <br>
+
class="gwd-img-zpsn" /></a><br
−
  <br>
+
style="font-family: Cambria;" />
−
  <img src="https://static.igem.org/mediawiki/2015/1/14/Logo_SA_2.png" class="gwd-img-n53q">
+
<br style="font-family: Cambria;" />
−
  <img src="https://static.igem.org/mediawiki/2015/thumb/e/e1/UMayor.png/800px-UMayor.png" class="gwd-img-kryd">
+
<br />
−
  <img src="https://static.igem.org/mediawiki/2015/thumb/4/4b/Maipu.png/405px-Maipu.png" class="gwd-img-ia0j">
+
−
<br>
+
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 13:02, 15 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pSB1C3
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:Parts.jpg
File:Circuito.png