Difference between revisions of "Team:San Andres/Parts"

 
(8 intermediate revisions by the same user not shown)
Line 2: Line 2:
  
 
<head data-gwd-animation-mode="quickMode">
 
<head data-gwd-animation-mode="quickMode">
 +
  <title>&lt;endnote&gt;&lt;head&gt; &lt;title&gt;&lt;endnote&gt;&lt;head&gt; &lt;meta&gt; &lt;title&gt;
 +
  </title>
 +
</head>
 +
 +
<body>
 
   <title>wiki</title>
 
   <title>wiki</title>
 
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
Line 14: Line 19:
 
       -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 
       -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
−
    background-image: url(https://static.igem.org/mediawiki/2015/8/8a/BBD7.jpg);
+
      background-image: url(https://static.igem.org/mediawiki/2015/8/8a/BBD7.jpg);
 
       background-color: rgb(198, 169, 117);
 
       background-color: rgb(198, 169, 117);
 
     }
 
     }
 
   </style>
 
   </style>
−
</head>
+
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
−
 
+
  <title></title>
−
<body class="gwd-body-kwce">
+
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 +
  <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 +
  <title></title>
 +
  <title>wiki</title>
 +
  <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 +
  <meta name="generator" content="Google Web Designer 1.3.2.0521">
 +
  <style type="text/css">
 +
    html, body {
 +
      width: 100%;
 +
      height: 100%;
 +
      margin: 0px;
 +
    }
 +
    body {
 +
      -webkit-transform: perspective(1400px) matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 +
      -webkit-transform-style: preserve-3d;
 +
      background-color: transparent;
 +
    }
 +
  </style>
 
   <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 
   <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
 
   <title></title>
 
   <title></title>
Line 129: Line 152:
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
 
       background-color: transparent;
 
       background-color: transparent;
 +
    }
 +
    .gwd-table-nb4m {
 +
      font-size: 24px;
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 +
      background-image: none;
 +
      background-color: rgb(255, 255, 255);
 
     }
 
     }
 
     .gwd-td-b6db {
 
     .gwd-td-b6db {
Line 509: Line 539:
 
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0.05, 0, 0, 1);
 
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0.05, 0, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
−
    }
 
−
    .gwd-img-l2p5 {
 
−
      position: absolute;
 
−
      width: 135px;
 
−
      left: -160px;
 
−
      top: 26px;
 
 
     }
 
     }
 
     .gwd-img-n53q {
 
     .gwd-img-n53q {
Line 535: Line 559:
 
     }
 
     }
 
     .gwd-a-36ww {
 
     .gwd-a-36ww {
−
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
+
       -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, -16.1, 0.05, 0, 1);
 
       -webkit-transform-style: preserve-3d;
 
       -webkit-transform-style: preserve-3d;
 
     }
 
     }
Line 546: Line 570:
 
       top: 349.384236453212px;
 
       top: 349.384236453212px;
 
       width: 250px;
 
       width: 250px;
 +
    }
 +
    .gwd-img-zpsn {
 +
      position: absolute;
 +
      width: 150px;
 +
      left: -165.73913043478px;
 +
      top: 49.3260869565235px;
 +
    }
 +
    .gwd-a-d01x {
 +
      -webkit-transform: matrix3d(1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1);
 +
      -webkit-transform-style: preserve-3d;
 +
    }
 +
    .gwd-table-10nh {
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 +
    }
 +
    .gwd-table-15ao {
 +
      -webkit-transform: translate3d(0px, -2px, 0px);
 +
      -webkit-transform-style: preserve-3d;
 
     }
 
     }
 
   </style>
 
   </style>
 
   <!--navigation menu -->
 
   <!--navigation menu -->
 
   <br>
 
   <br>
−
   <div class="gwd-div-2ed9"></div>
+
   <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-15ao">
−
  <div class="gwd-div-7xzo gwd-a-1thu">
+
    <tbody>
−
    <h1>Parts&nbsp;</h1>
+
      <tr heigth="15px">
−
    <big>Throughout the project we started to learn the fundamental
+
      </tr>
 +
      <tr heigth="75px">
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>
 +
        </td>
 +
        <td style="border: 3px solid black;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);"> Modeling</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'"><a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
  <table style="width: 968px; height: 34px;" class="gwd-table-nb4m editable gwd-table-10nh">
 +
    <tbody>
 +
      <tr heigth="15px">
 +
      </tr>
 +
      <tr heigth="75px">
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);">Notebook</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Attributions" style="color: rgb(0, 0, 0);"> Attributions</a>
 +
        </td>
 +
        <td style="border: 3px solid #1d1003;" align="center" bgcolor="#ffee1b" height="30" onmouseout="this.bgColor='#ffee1b'" onmouseover="this.bgColor='#6bb402'">
 +
          <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
<div class="gwd-div-2ed9"></div>
 +
<div class="gwd-div-7xzo gwd-a-1thu">
 +
<h1 style="font-family: Algerian;"><big>Parts&nbsp;</big></h1>
 +
<div style="text-align: justify; font-family: Cambria;"><big>Throughout
 +
the project we started to learn the fundamental
 
principles of synthetic biology to get to work on our plasmid with
 
principles of synthetic biology to get to work on our plasmid with
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
this we going to insert in an e. coli the parts (Biobricks) needed to
 
this we going to insert in an e. coli the parts (Biobricks) needed to
−
make our future bacteria can degrade gluten. The parts are:<br>
+
make our future bacteria can degrade gluten. The parts are:</big><br />
−
</big>
+
</div>
−
    <ul>
+
<ul style="font-family: Cambria;">
−
      <li><big><span style="font-family: 'Arial','sans-serif';"><strong style="font-weight: bold;">Promoter
+
  <li style="text-align: justify;"><big><strong
−
     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a href="http://parts.igem.org/Part:BBa_J23119"><span style="font-weight: bold;">)</span></a>:
+
style="font-weight: bold;">Promoter
−
    </span>Constitutive
+
     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a
 +
href="http://parts.igem.org/Part:BBa_J23119"><span
 +
style="font-weight: bold;">)</span></a>:
 +
Constitutive
 
promoter (which works permanently) that is give in the relative
 
promoter (which works permanently) that is give in the relative
 
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
 
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
−
      </li>
+
  </li>
−
      <li><big><strong style="font-weight: bold;">RBS</strong><strong style="font-weight: bold;">
+
  <li style="text-align: justify;"><big><strong
 +
style="font-weight: bold;">RBS</strong><strong
 +
style="font-weight: bold;">
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
Synthetic RBS with uplifting sequence</big>.</li>
 
Synthetic RBS with uplifting sequence</big>.</li>
−
      <li><big><strong>Vector:<span style="font-weight: normal;"> <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
+
  <li style="text-align: justify;"><big><strong>Vector:<span
−
      </li>
+
style="font-weight: normal;"> <a
−
      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Coding Region:
+
href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
−
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
+
  </li>
 +
  <li style="text-align: justify;"><big><strong>Coding
 +
Region:
 +
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</strong>
 
It
 
It
 
degrades gluten,
 
degrades gluten,
 
celiac disease leading cause. Enzyme generated by rational mutation for
 
celiac disease leading cause. Enzyme generated by rational mutation for
−
the active site of it. It was created by the team IGEM <a href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
+
the active site of it. It was created by the team IGEM <a
 +
href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
 
2011.</a></big>
 
2011.</a></big>
−
      </li>
+
  </li>
−
      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Reporter: RFP
+
  <li style="text-align: justify;"><big><strong>Reporter:
−
     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span style="font-family: Arial,sans-serif;"> Red f</span>luorescence
+
RFP
 +
     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</strong>
 +
Red fluorescence
 
protein.</big>
 
protein.</big>
−
      </li>
+
  </li>
−
      <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
+
  <li style="text-align: justify;"><big><strong>Terminator
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
−
    </span>Dual terminator consisting of
+
Dual terminator consisting of
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
transcription</big>.</li>
 
transcription</big>.</li>
−
    </ul>
+
</ul>
−
    <div style="text-align: center;">
+
<div style="text-align: center; font-family: Cambria;">
−
      <img alt="File:Parts.jpg" src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg" height="59" width="288">
+
<img alt="File:Parts.jpg"
−
      <br>
+
src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg"
−
      <img alt="File:Circuito.jpg" src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg" height="309" width="800">
+
height="59" width="288" /><br />
−
      <br>
+
<img
−
      <div style="text-align: left;"><big>This is a
+
src="https://static.igem.org/mediawiki/2015/thumb/3/34/Circuito.png/800px-Circuito.png"
−
graphic model of as it has be our plasmid where we can visualize the
+
alt="File:Circuito.png" />&nbsp;<br />
−
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
+
</div>
−
joined by means of prefixes and suffixes that indicate the locations of
+
<big style="font-family: Cambria;">
−
court.</big>
+
</big></div>
−
        <br>
+
<br style="font-family: Cambria;" />
−
        <br>
+
<a style="font-family: Cambria;"
−
        <div style="text-align: center;">
+
href="https://2015.igem.org/Main_Page"
−
          <img alt="File:Plasmido.jpg" src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg" height="600" width="664">
+
class="gwd-a-3xpx gwd-a-36ww">
−
        </div>
+
<img
−
      </div>
+
style="border: 0px solid ; width: 150px; height: 150px; left: 989px; top: 49px;"
−
    </div>
+
alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
−
    <big><br>
+
class="gwd-img-kwqf" /></a><a
−
</big>
+
style="font-family: Cambria;"
−
  </div>
+
href="https://igem.org/Team_List?year=2015"
−
  <br>
+
class="gwd-a-3xpx gwd-a-d01x"><img
−
  <a href="https://2015.igem.org/Main_Page" class="gwd-a-3xpx gwd-a-36ww">
+
src="https://static.igem.org/mediawiki/2015/thumb/8/85/Logo_Definitvo.png/654px-Logo_Definitvo.png"
−
    <img style="border: 0px solid ; width: 150px; height: 150px; top: 35px; left: 989px;" alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" class="gwd-img-kwqf">
+
class="gwd-img-zpsn" /></a><br
−
  </a>
+
style="font-family: Cambria;" />
−
  <a href="https://igem.org/Team_List?year=2015" class="gwd-a-3xpx">
+
<br style="font-family: Cambria;" />
−
    <img src="https://static.igem.org/mediawiki/2015/thumb/9/94/Logo43.png/454px-Logo43.png" class="gwd-img-l2p5">
+
<br />
−
  </a>
+
−
  <br>
+
−
  <br>
+
−
  <div class="gwd-div-t5nw gwd-div-fola">
+
−
    <style>
+
−
      body {
+
−
        font-family: Arial;
+
−
      }
+
−
      nav ul {
+
−
        list-style: none;
+
−
        padding: 0px;
+
−
      }
+
−
      nav li {
+
−
        line-height: 3rem;
+
−
        position: relative;
+
−
        width: 190px;
+
−
      }
+
−
      nav li ul {
+
−
        position: absolute;
+
−
      }
+
−
      nav a {
+
−
        color: rgb(0, 0, 0);
+
−
        display: block;
+
−
        padding: 0px 2.5em;
+
−
        text-decoration: none;
+
−
        transition: 0.3s;
+
−
        -webkit-transition: 0.3s;
+
−
      }
+
−
      nav a:hover {
+
−
        background: rgb(255, 186, 0);
+
−
      }
+
−
      nav li ul {
+
−
        display: none;
+
−
      }
+
−
      nav li:hover ul {
+
−
        display: block;
+
−
      }
+
−
      nav > ul {
+
−
        display: table;
+
−
        width: 100%;
+
−
        background: rgb(255, 166, 0);
+
−
      }
+
−
      nav > ul > li {
+
−
        float: left;
+
−
      }
+
−
      nav li li {
+
−
        max-height: 0px;
+
−
        transition: all 0.1s;
+
−
        -webkit-transition: all 0.1s;
+
−
        overflow: hidden;
+
−
        width: 200px;
+
−
        background: rgb(237, 173, 0);
+
−
      }
+
−
      nav li:hover > ul > li {
+
−
        max-height: 500px;
+
−
        overflow: visible;
+
−
      }
+
−
      nav > ul > li > ul {
+
−
        top: 100%;
+
−
      }
+
−
      .gwd-nav-nvls {
+
−
        -webkit-transform: translate3d(-13.57px, 349.01px, 0px);
+
−
        -webkit-transform-style: preserve-3d;
+
−
      }
+
−
      .gwd-div-fola {
+
−
        -webkit-transform: translate3d(-7.67px, -1793.66px, 0px);
+
−
        -webkit-transform-style: preserve-3d;
+
−
      }
+
−
    </style>
+
−
    <nav class="gwd-nav-nvls">
+
−
      <ul>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres">Home</a>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Team">Team</a>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Description">Project</a>
+
−
          <ul>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Parts">Parts</a>
+
−
            </li>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Modeling">Modeling</a>
+
−
            </li>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Results">Results</a>
+
−
            </li>
+
−
          </ul>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Notebook">Notebook</a>
+
−
          <ul>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Practices">Human Practices</a>
+
−
            </li>
+
−
            <li><a href="https://2015.igem.org/Team:San_Andres/Design">Future Projections</a>
+
−
            </li>
+
−
          </ul>
+
−
        </li>
+
−
        <li><a href="https://2015.igem.org/Team:San_Andres/Collaborations">Collaborations</a>
+
−
        </li>
+
−
      </ul>
+
−
    </nav>
+
−
  </div>
+
 
</body>
 
</body>
−
 
 
</html>
 
</html>

Latest revision as of 13:02, 15 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pSB1C3
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:Parts.jpg
File:Circuito.png