Difference between revisions of "Team:Bielefeld-CeBiTec/Organisms"
m |
m |
||
| Line 28: | Line 28: | ||
</div> | </div> | ||
| − | <div class="Subtitle"> | + | <div class="Subtitle" id="Ecoli"> |
<h2><i>Escherichia coli</i></h2> | <h2><i>Escherichia coli</i></h2> | ||
</div> | </div> | ||
Revision as of 21:36, 15 September 2015
Organisms
...
Escherichia coli
We used following strains of E. coli during our project:
- KRX
- Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.
- ER2566 (T7 Express Competent E. coli)
- Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm]
Bacillus subtilis
In our project we used B. subtilis strain 168 with genotype trpC2. You can find further information about the origin of this strain in Zeigler et al.
Agrobacterium tumefaciens
We isolated some sequence from A. tumefaciens C58.1. For detailed information about this organism please be referred to Wood et al. 2001.
References
Wood, D. W.; Setubal, J. C.; Kaul, R.; Monks, D. E.; Kitajima, J. P.; Okura, V. K. et al. (2001): The genome of the natural genetic engineer Agrobacterium tumefaciens C58. In: Science (New York, N.Y.) 294 (5550), S. 2317–2323. DOI: 10.1126/science.1066804.
Zeigler, Daniel R.; Prágai, Zoltán; Rodriguez, Sabrina; Chevreux, Bastien; Muffler, Andrea; Albert, Thomas et al. (2008): The origins of 168, W23, and other Bacillus subtilis legacy strains. In: Journal of bacteriology 190 (21), S. 6983–6995. DOI: 10.1128/JB.00722-08.
