Difference between revisions of "Team:HZAU-China/InterLab/Results"

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       <h1></br></br></br>Overview</h1></br>
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       <h1></br></br></br>Results</h1></br>
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       <p>As our team is competing this year's competition, we also required to participate in the iGEM 2015 Measurement Interlab study. The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around world.</p>
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<h3>1. Here are results of per-experiment.</h3>
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      </br><p>The three genetic devices differ in their promotors :</p>
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       <p>The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours. </p>
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      <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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      <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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<img src="https://static.igem.org/mediawiki/2015/8/82/Team_HZAU-China_interp1.png" width="750px" height="300px">
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      <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p></br>
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      <p>We transformed the three devices into competent cell (DH 5 alpha) and measured fluorescence as well as optical density of the LB medium at the suitable period (We did pre-experiment before formal experiment for confirming the suitable period as well as the dilution factor), using a plate reader, respectively. We hypothesized that the GFP expression quality of the three devices level is Device 1, Device 2, Device 3, respectively. And our final result accord with our hypothesis.</p>
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<p class="zhushi">Fig.1 Growth curve of per-experiment</p>
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<p>As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.</p>
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Revision as of 03:05, 16 September 2015

Mixed-Reality Cell

Bidirectinal coupling between real and virtual bio-oscillator




Results


1. Here are results of per-experiment.

The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours.


Fig.1 Growth curve of per-experiment

As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.





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