Difference between revisions of "Team:Bielefeld-CeBiTec/Protocols"
(Created page with "{{Bielefeld-CeBiTec/Header}} <html> <script> $(document).ready(function(){ $("#button1").click(function(){ $(".panel-collapse").collapse("show"); }); }); $(document).re...") |
|||
| Line 96: | Line 96: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
| Line 108: | Line 109: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
| Line 128: | Line 130: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
| Line 135: | Line 138: | ||
</div> | </div> | ||
<div id="collapseEight" class="panel-collapse collapse"> | <div id="collapseEight" class="panel-collapse collapse"> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<div class="panel-body"> | <div class="panel-body"> | ||
<p>...</p> | <p>...</p> | ||
Revision as of 18:49, 22 June 2015
...
...
...
...
- Thaw 50 µl electrocompetent E. coli cells on ice, dilute with icecold 50 µl glycerine (10%) if necessary
- Add 0.5-5 µl plasmid to 50 µl electrocompetent cells
- Store cells on ice for 1 minute
- Electroporate at U = 2.5 kV, C = 25 µF, R = 400 Ω
- Transfer transformation reaction to 450 µl SOC-Medium and incubate 1 h at 37 °C
- Plate on selective LB-Medium
- Incubate over night at 37 °C