Difference between revisions of "Team:OUC-China"

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Revision as of 16:34, 16 September 2015

<!DOCTYPE html> Team:OUC-China

how does it work?

This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.

step 1

Our Magthermocoli contains 2 core components: magnetic receiver & thermosensitive regulator.

step 2

Induced by electromagnetic field，magnetic receiver would be heated, thus raise the ambient temperature.

step 3

In response to the change of temperature, thermosensitive regulator would initiate GFP (or RFP) expression.

Magnetic Receiver

For magnetic receiver,we chose Ferritin, the iron-storage protein in organisms, which could synthesizeferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core would be heated, raising the ambient temperature.

In this session, we explored measurement techniques for the valuation of magnetism、biomineralization and Protein concentration per cell.

More details

Thermosensitive Regulator

For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.

RNA thermometer is a structured RNA which could expose RBS only at appropriate temperature. In this session, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.

More details

Thermosensitive T7 RNA polymerase is a normal T7 RNA polymerase at whose selected locus was inserted by a temperature-sensitive intein, which could self-splice at specific temperature.

More details