Difference between revisions of "Team:San Andres/Notebook"
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<table style="width: 968px; height: 34px;" | <table style="width: 968px; height: 34px;" | ||
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style="font-family: Cambria;" lang="EN-US"><span | style="font-family: Cambria;" lang="EN-US"><span | ||
style="font-weight: bold;">January:</span> | style="font-weight: bold;">January:</span> | ||
| − | Cultivation of Biobricks, extraction plasmidial and double digestion. </span></big></li> | + | Cultivation of Biobricks, extraction plasmidial and double digestion.</span></big></li> |
| + | <br> | ||
</ul> | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
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<br> | <br> | ||
<img style="width: 642px; height: 132px;" alt="File:Tabla.png" | <img style="width: 642px; height: 132px;" alt="File:Tabla.png" | ||
| − | src="https://static.igem.org/mediawiki/2015/e/ee/Tabla.png"></div> | + | src="https://static.igem.org/mediawiki/2015/e/ee/Tabla.png"><br> |
| + | <br> | ||
| + | </div> | ||
<ul> | <ul> | ||
<li><big><span style=""><span | <li><big><span style=""><span | ||
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Preparation of methodologies and ligation of KumaMax with RFP.<span | Preparation of methodologies and ligation of KumaMax with RFP.<span | ||
style=""> </span></span></big></li> | style=""> </span></span></big></li> | ||
| + | <br> | ||
</ul> | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
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style="width: 557px; height: 374px;" alt="File:Methodology.jpg" | style="width: 557px; height: 374px;" alt="File:Methodology.jpg" | ||
src="https://static.igem.org/mediawiki/2015/thumb/3/34/Methodology.jpg/800px-Methodology.jpg" | src="https://static.igem.org/mediawiki/2015/thumb/3/34/Methodology.jpg/800px-Methodology.jpg" | ||
| − | srcset="/wiki/images/thumb/3/34/Methodology.jpg/1200px-Methodology.jpg 1.5x, /wiki/images/3/34/Methodology.jpg 2x"></div> | + | srcset="/wiki/images/thumb/3/34/Methodology.jpg/1200px-Methodology.jpg 1.5x, /wiki/images/3/34/Methodology.jpg 2x"><br> |
| + | <br> | ||
| + | </div> | ||
<ul> | <ul> | ||
<li><big><span style=""><span | <li><big><span style=""><span | ||
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</span></span><span style="" lang="EN-US"><span | </span></span><span style="" lang="EN-US"><span | ||
style="font-weight: bold;">March:</span> | style="font-weight: bold;">March:</span> | ||
| − | Presentation of the project to CORFO.<span style=""> | + | Presentation of the project to CORFO.<span style=""> </span></span></big></li> |
| − | + | <br> | |
</ul> | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
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alt="File:Igem marzo 2015.jpg" | alt="File:Igem marzo 2015.jpg" | ||
src="https://static.igem.org/mediawiki/2015/thumb/8/8f/Igem_marzo_2015.jpg/800px-Igem_marzo_2015.jpg" | src="https://static.igem.org/mediawiki/2015/thumb/8/8f/Igem_marzo_2015.jpg/800px-Igem_marzo_2015.jpg" | ||
| − | srcset="/wiki/images/8/8f/Igem_marzo_2015.jpg 1.5x, /wiki/images/8/8f/Igem_marzo_2015.jpg 2x"></div> | + | srcset="/wiki/images/8/8f/Igem_marzo_2015.jpg 1.5x, /wiki/images/8/8f/Igem_marzo_2015.jpg 2x"><br> |
| + | <br> | ||
| + | </div> | ||
<ul> | <ul> | ||
<li><big><span style=""><span | <li><big><span style=""><span | ||
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</span></span><span style="" lang="EN-US"><span | </span></span><span style="" lang="EN-US"><span | ||
style="font-weight: bold;">May:</span> | style="font-weight: bold;">May:</span> | ||
| − | Samples are lost. Restart procedures.<span style=""> | + | Samples are lost. Restart procedures.<span style=""> </span></span></big></li> |
| − | + | <br> | |
</ul> | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
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<img style="width: 366px; height: 274px;" | <img style="width: 366px; height: 274px;" | ||
alt="File:Biobricks2.jpg" | alt="File:Biobricks2.jpg" | ||
| − | src="https://static.igem.org/mediawiki/2015/e/ee/Biobricks2.jpg"></div> | + | src="https://static.igem.org/mediawiki/2015/e/ee/Biobricks2.jpg"><br> |
| + | <br> | ||
| + | </div> | ||
<ul> | <ul> | ||
<li><big><span style=""><span | <li><big><span style=""><span | ||
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</span></span></span><span style="" | </span></span></span><span style="" | ||
lang="EN-US"><span style="font-weight: bold;">June:</span> | lang="EN-US"><span style="font-weight: bold;">June:</span> | ||
| − | Double digestion and links which fail.<span style=""> | + | Double digestion and links which fail.<span style=""> </span></span></big></li> |
| − | + | <br> | |
</ul> | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
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height="216" width="302"> <img | height="216" width="302"> <img | ||
style="width: 417px; height: 216px;" alt="File:Electro5.jpg" | style="width: 417px; height: 216px;" alt="File:Electro5.jpg" | ||
| − | src="https://static.igem.org/mediawiki/2015/b/bf/Electro5.jpg"></div> | + | src="https://static.igem.org/mediawiki/2015/b/bf/Electro5.jpg"><br> |
| + | <br> | ||
| + | </div> | ||
<ul> | <ul> | ||
<li><big><span style=""><span | <li><big><span style=""><span | ||
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</span></span></span><span style="" | </span></span></span><span style="" | ||
lang="EN-US"><span style="font-weight: bold;">July:</span> | lang="EN-US"><span style="font-weight: bold;">July:</span> | ||
| − | Apply plan of ligations. | + | Apply plan of ligations.</span></big></li> |
| − | + | <br> | |
</ul> | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
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style="" lang="EN-US"><span style=""></span></span></big><br> | style="" lang="EN-US"><span style=""></span></span></big><br> | ||
<br> | <br> | ||
| − | <div style="text-align: left;"><big><span style=""><span | + | <div style="text-align: left;"> |
| + | <ul> | ||
| + | <li><big><span style=""><span | ||
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> <span | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> <span | ||
style="font-weight: bold;"> | style="font-weight: bold;"> | ||
| − | </span></span></span><span style="" | + | </span></span></span><span style="" |
lang="EN-US"><span style="font-weight: bold;">August:</span> | lang="EN-US"><span style="font-weight: bold;">August:</span> | ||
All failed again. Change in methodology for the assambly standar. | All failed again. Change in methodology for the assambly standar. | ||
| − | Restart procedures.<span style=""> </span></span></big>< | + | Restart procedures.<span style=""> </span></span></big></li> |
| + | </ul> | ||
<br> | <br> | ||
</div> | </div> | ||
| − | <img style="width: | + | <img style="width: 341px; height: 169px;" |
alt="File:Electro9.jpg" | alt="File:Electro9.jpg" | ||
src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Electro9.jpg/800px-Electro9.jpg" | src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Electro9.jpg/800px-Electro9.jpg" | ||
srcset="/wiki/images/1/1c/Electro9.jpg 1.5x, /wiki/images/1/1c/Electro9.jpg 2x"> | srcset="/wiki/images/1/1c/Electro9.jpg 1.5x, /wiki/images/1/1c/Electro9.jpg 2x"> | ||
| − | </div> | + | <img class="shrinkToFit" |
| + | alt="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" | ||
| + | src="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" | ||
| + | style="border: medium none ; vertical-align: middle; width: 335px; height: 290px;"><br> | ||
| + | <br> | ||
| + | </div> | ||
<ul> | <ul> | ||
<li><big><span style="font-family: Cambria;" | <li><big><span style="font-family: Cambria;" | ||
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style="font-weight: bold;">September:</span> | style="font-weight: bold;">September:</span> | ||
Finally works the KMX - RFP ligation and the plasmid.</span></big></span></li> | Finally works the KMX - RFP ligation and the plasmid.</span></big></span></li> | ||
| + | <br> | ||
</ul> | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
Latest revision as of 21:00, 16 September 2015
| Home | Team | Project | Parts | Modeling | Results |
| Notebook | Human Practices | Future Projections | Attributions | Collaborations |
Notebook
This
project was initiated in April of the year 2014 and in this time we
have
acquired new experiences like to know students from other schools and
communes
with our same interests, also with our same age range, which goes
promoting
inter-school knowledges. Also this project has served as a guide for
the
future, helping us to understand University life through various clases
of the
sience fields, as in the case of molecular biology, where it taught us
the
basics of this, which helped us to understand concepts like what is an
plasmid?, and how use it, things which helped us to generate an idea of
how to
solve a problem. Then
we learned various techniques from laboratories to handle the
bacterias,stuff of laboratory, laboratory's machines and biobricks,
essensial
things for our work, and like that start own project, in this case
about celiac
disease.
Lab (2015)
- January: Cultivation of Biobricks, extraction plasmidial and double digestion.
- February: Preparation of methodologies and ligation of KumaMax with RFP.
- March: Presentation of the project to CORFO.
- May: Samples are lost. Restart procedures.
- June: Double digestion and links which fail.
- July: Apply plan of ligations.
- August: All failed again. Change in methodology for the assambly standar. Restart procedures.
- September: Finally works the KMX - RFP ligation and the plasmid.
