Difference between revisions of "Team:San Andres/Notebook"

 
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       <a href="https://2015.igem.org/Team:San_Andres/Team"
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  onmouseout="this.bgColor='#ffee1b'"
−
onmouseout="this.bgColor='#fcff00'" align="center"
+
  onmouseover="this.bgColor='#6bb402'" align="center"
−
bgcolor="#fcff00" height="45">
+
  bgcolor="#ffee1b" height="30">
−
      <a href="https://2015.igem.org/Team:San_Andres/Experiments"
+
−
style="color: rgb(0, 0, 0);"> Enzymes</a>
+
−
      </td>
+
−
      <td style="border: 2px solid black;"
+
−
  onmouseover="this.bgColor='#ffba00'"
+
−
  onmouseout="this.bgColor='#fcff00'" align="center"
+
−
  bgcolor="#fcff00" height="45">
+
 
       <a href="https://2015.igem.org/Team:San_Andres/Parts"
 
       <a href="https://2015.igem.org/Team:San_Andres/Parts"
 
  style="color: rgb(0, 0, 0);"> Parts</a>
 
  style="color: rgb(0, 0, 0);"> Parts</a>
 
       </td>
 
       </td>
−
       <td style="border: 2px solid black;"
+
       <td style="border: 3px solid rgb(29, 16, 3);"
−
  onmouseover="this.bgColor='#ffba00'"
+
  onmouseout="this.bgColor='#ffee1b'"
−
  onmouseout="this.bgColor='#fcff00'" align="center"
+
  onmouseover="this.bgColor='#6bb402'" align="center"
−
  bgcolor="#fcff00" height="45">
+
  bgcolor="#ffee1b" height="30">
 
       <a href="https://2015.igem.org/Team:San_Andres/Modeling"
 
       <a href="https://2015.igem.org/Team:San_Andres/Modeling"
−
  style="color: rgb(0, 0, 0);"> Metodology</a>
+
  style="color: rgb(0, 0, 0);"> Modeling</a>
 
       </td>
 
       </td>
−
       <td style="border: 2px solid black;"
+
       <td style="border: 3px solid rgb(29, 16, 3);"
−
  onmouseover="this.bgColor='#ffba00'"
+
  onmouseout="this.bgColor='#ffee1b'"
−
  onmouseout="this.bgColor='#fcff00'" align="center"
+
  onmouseover="this.bgColor='#6bb402'" align="center"
−
  bgcolor="#fcff00" height="45">
+
  bgcolor="#ffee1b" height="30"><a
−
      <a href="https://2015.igem.org/Team:San_Andres/Results"
+
href="https://2015.igem.org/Team:San_Andres/Results"
−
  style="color: rgb(0, 0, 0);"> Results </a>
+
  style="color: rgb(0, 0, 0);"> Results</a>
 
       </td>
 
       </td>
−
       <td style="border: 2px solid black;"
+
    </tr>
−
  onmouseover="this.bgColor='#ffba00'"
+
  </tbody>
−
  onmouseout="this.bgColor='#fcff00'" align="center"
+
</table>
−
  bgcolor="#fcff00" height="45">
+
<table style="width: 968px; height: 34px;"
 +
class="gwd-table-nb4m editable gwd-table-10nh">
 +
  <tbody>
 +
    <tr heigth="15px">
 +
    </tr>
 +
    <tr heigth="75px">
 +
       <td style="border: 3px solid rgb(29, 16, 3);"
 +
onmouseout="this.bgColor='#ffee1b'"
 +
  onmouseover="this.bgColor='#6bb402'" align="center"
 +
bgcolor="#ffee1b" height="30">
 +
      <a href="https://2015.igem.org/Team:San_Andres/Notebook"
 +
style="color: rgb(0, 0, 0);">Notebook</a>
 +
      </td>
 +
      <td style="border: 3px solid rgb(29, 16, 3);"
 +
  onmouseout="this.bgColor='#ffee1b'"
 +
onmouseover="this.bgColor='#6bb402'" align="center"
 +
bgcolor="#ffee1b" height="30">
 +
      <a href="https://2015.igem.org/Team:San_Andres/Practices"
 +
style="color: rgb(0, 0, 0);"> Human Practices</a>
 +
      </td>
 +
      <td style="border: 3px solid rgb(29, 16, 3);"
 +
onmouseout="this.bgColor='#ffee1b'"
 +
onmouseover="this.bgColor='#6bb402'" align="center"
 +
bgcolor="#ffee1b" height="30">
 +
      <a href="https://2015.igem.org/Team:San_Andres/Design"
 +
style="color: rgb(0, 0, 0);"> Future Projections</a>
 +
      </td>
 +
      <td style="border: 3px solid rgb(29, 16, 3);"
 +
onmouseout="this.bgColor='#ffee1b'"
 +
onmouseover="this.bgColor='#6bb402'" align="center"
 +
bgcolor="#ffee1b" height="30">
 +
      <a href="https://2015.igem.org/Team:San_Andres/Attributions"
 +
style="color: rgb(0, 0, 0);"> Attributions</a>
 +
      </td>
 +
      <td style="border: 3px solid rgb(29, 16, 3);"
 +
onmouseout="this.bgColor='#ffee1b'"
 +
onmouseover="this.bgColor='#6bb402'" align="center"
 +
  bgcolor="#ffee1b" height="30">
 
       <a href="https://2015.igem.org/Team:San_Andres/Collaborations"
 
       <a href="https://2015.igem.org/Team:San_Andres/Collaborations"
−
  style="color: rgb(0, 0, 0);"> Future Projections </a>
+
  style="color: rgb(0, 0, 0);"> Collaborations</a>
 
       </td>
 
       </td>
−
      <td align="center">
 
−
      <a href="https://igem.org/Team_List?year=2015"><img
 
−
src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
 
−
width="55"></a></td>
 
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>
Line 274: Line 731:
 
<div class="gwd-div-2ed9"></div>
 
<div class="gwd-div-2ed9"></div>
 
<div class="gwd-div-7xzo gwd-a-1thu">
 
<div class="gwd-div-7xzo gwd-a-1thu">
−
<h1>Statistics</h1>
+
<h1><big style="font-family: Algerian; font-weight: normal;">Notebook</big><br>
−
<div style="text-align: left;"><big>Europe have the
+
</h1>
−
contries with more celiacs internationally, also they are the places
+
<div style="text-align: justify; font-family: Cambria;"><big><span
−
with more deaths by this pathology and the regions than more consuming
+
style="color: black;">This
−
wheat in the world.</big><br>
+
project was initiated in April of the year 2014 and in this time we
 +
have
 +
acquired new experiences like to know students from other schools and
 +
communes
 +
with our same interests, also with our same age range, which goes
 +
promoting
 +
inter-school knowledges. Also this project has served as a guide for
 +
the
 +
future, helping us to understand University life through various clases
 +
of the
 +
sience fields, as in the case of molecular biology, where it taught us
 +
the
 +
basics of this, which helped us to understand concepts like what is an
 +
plasmid?, and how use it, things which helped us to generate an idea of
 +
how to
 +
solve a problem.</span> <span style="color: black;">Then
 +
we learned various techniques from laboratories to handle the
 +
bacterias,stuff of laboratory, laboratory's machines and biobricks,
 +
essensial
 +
things for our work, and like that start own project, in this case
 +
about celiac
 +
disease.</span></big><span style="color: black;"><o:p></o:p></span><br>
 +
<br>
 +
<span style="color: black;"></span><span
 +
style="color: black;"><o:p></o:p></span></div>
 +
<div style="text-align: center; font-family: Cambria;">
 +
<div style="text-align: justify;">
 
<div style="text-align: center;"><img
 
<div style="text-align: center;"><img
−
  alt="File:Stastics.png"
+
  style="width: 462px; height: 308px;" alt="File:Lab 800.jpg"
−
  src="https://static.igem.org/mediawiki/2015/0/0d/Stastics.png"
+
  src="https://static.igem.org/mediawiki/2015/0/01/Lab_800.jpg">&nbsp;
−
height="488" width="651"><br>
+
<img style="width: 475px; height: 308px;"
−
<div style="text-align: left;"><big>Since the main
+
  alt="File:Bio molecular.png"
−
gene pool of people with this mutation originated in Northern Europe,
+
  src="https://static.igem.org/mediawiki/2015/3/39/Bio_molecular.png">
−
all of their descendents throughout North America, Australia, South
+
−
Africa, etc. are at a higher risk for inheriting this genetic
+
−
predisposition to the disease.&nbsp;</big><span
+
−
  id="result_box" class="" lang="en"><big><span
+
−
  class="hps">For example</span> <span class="hps">in
+
−
the United States</span><span>,</span> <span
+
−
class="hps">the prevalence</span> <span class="hps">of
+
−
celiac disease</span> <span class="hps">by</span>
+
−
<span class="hps">ethnic</span> <span class="hps">race</span>
+
−
</big><span class="hps"><big>is:<br>
+
−
</big><br>
+
 
<br>
 
<br>
−
</span></span>
+
<big><big><br style="font-weight: bold;">
 +
</big></big>
 +
<div style="text-align: left;"><big><big><span
 +
style="font-weight: bold;">Lab (2015)</span></big></big><br>
 +
<ul>
 +
  <li><big><span style="font-family: Symbol;"
 +
lang="EN-US"><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal; font-family: Cambria;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
    </span></span></span><span
 +
style="font-family: Cambria;" lang="EN-US"><span
 +
style="font-weight: bold;">January:</span>
 +
Cultivation of Biobricks, extraction plasmidial and double digestion.</span></big></li>
 +
  <br>
 +
</ul>
 
<div style="text-align: center;"><img
 
<div style="text-align: center;"><img
−
  alt="File:Celiac-Disease US.png"
+
  style="width: 394px; height: 234px;" alt="Biobricks.jpg"
−
  src="https://static.igem.org/mediawiki/2015/thumb/5/5a/Celiac-Disease_US.png/800px-Celiac-Disease_US.png"
+
  src="https://static.igem.org/mediawiki/2015/thumb/c/c5/Biobricks.jpg/300px-Biobricks.jpg"
−
  height="445" width="800"><br>
+
  class="thumbimage"
 +
srcset="/wiki/images/c/c5/Biobricks.jpg 1.5x, /wiki/images/c/c5/Biobricks.jpg 2x">&nbsp;<img
 +
style="width: 513px; height: 235px;"
 +
alt="https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg"><br>
 
<br>
 
<br>
−
<div style="text-align: left;"><br>
+
<img style="width: 642px; height: 132px;" alt="File:Tabla.png"
−
<img src="https://static.igem.org/mediawiki/2015/7/75/Celiaco.jpg"
+
src="https://static.igem.org/mediawiki/2015/e/ee/Tabla.png"><br>
−
alt="celiaco.jpg" title="celiaco.jpg"
+
−
style="width: 365px; height: 363px; float: left;"><br>
+
 
<br>
 
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">
 +
    </span></span></span><span style=""
 +
lang="EN-US"><span style="font-weight: bold;">February:</span>
 +
Preparation of methodologies and ligation of KumaMax with RFP.<span
 +
style="">&nbsp;&nbsp;</span></span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
alt="File:Electro2jpg.png"
 +
src="https://static.igem.org/mediawiki/2015/e/e2/Electro2jpg.png"
 +
height="374" width="278"> <img
 +
style="width: 557px; height: 374px;" alt="File:Methodology.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/3/34/Methodology.jpg/800px-Methodology.jpg"
 +
srcset="/wiki/images/thumb/3/34/Methodology.jpg/1200px-Methodology.jpg 1.5x, /wiki/images/3/34/Methodology.jpg 2x"><br>
 
<br>
 
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
    </span></span><span style="" lang="EN-US"><span
 +
style="font-weight: bold;">March:</span>
 +
Presentation of the project to CORFO.<span style="">&nbsp;&nbsp;</span></span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 374px; height: 299px;"
 +
alt="File:120315 IGEM 154 copia.jpg"
 +
src="https://static.igem.org/mediawiki/2015/f/f3/120315_IGEM_154_copia.jpg">
 +
<img style="width: 397px; height: 298px;"
 +
alt="File:Igem marzo 2015.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/8/8f/Igem_marzo_2015.jpg/800px-Igem_marzo_2015.jpg"
 +
srcset="/wiki/images/8/8f/Igem_marzo_2015.jpg 1.5x, /wiki/images/8/8f/Igem_marzo_2015.jpg 2x"><br>
 
<br>
 
<br>
−
<div style="text-align: center;"><big>&nbsp;And
+
</div>
−
in our country Chile:<br>
+
<ul>
−
</big></div>
+
  <li><big><span style=""><span
−
<ul style="text-align: center;">
+
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
−
  <li><big>The probability of having celiac disease is 1
+
    </span></span><span style="" lang="EN-US"><span
−
each 265.</big></li>
+
style="font-weight: bold;">May:</span>
−
  <li><big>The celiac patients represent 15% of the Chilean
+
Samples are lost. Restart procedures.<span style="">&nbsp;&nbsp;&nbsp;</span></span></big></li>
−
population.</big></li>
+
   <br>
−
   <li><big>Most die by cancer without really knowing their
+
−
status.</big></li>
+
 
</ul>
 
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 503px; height: 276px;" alt="File:Electro3.png"
 +
src="https://static.igem.org/mediawiki/2015/thumb/c/c8/Electro3.png/800px-Electro3.png"
 +
srcset="/wiki/images/c/c8/Electro3.png 1.5x, /wiki/images/c/c8/Electro3.png 2x">
 +
<img style="width: 366px; height: 274px;"
 +
alt="File:Biobricks2.jpg"
 +
src="https://static.igem.org/mediawiki/2015/e/ee/Biobricks2.jpg"><br>
 +
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">&nbsp;
 +
    </span></span></span><span style=""
 +
lang="EN-US"><span style="font-weight: bold;">June:</span>
 +
Double digestion and links which fail.<span style="">&nbsp;&nbsp;</span></span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
alt="File:Electro4.png"
 +
src="https://static.igem.org/mediawiki/2015/5/57/Electro4.png"
 +
height="216" width="302"> <img
 +
style="width: 417px; height: 216px;" alt="File:Electro5.jpg"
 +
src="https://static.igem.org/mediawiki/2015/b/bf/Electro5.jpg"><br>
 +
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">&nbsp;&nbsp;
 +
    </span></span></span><span style=""
 +
lang="EN-US"><span style="font-weight: bold;">July:</span>
 +
Apply plan of ligations.</span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 311px; height: 288px;" alt="File:Electro6.jpg"
 +
src="https://static.igem.org/mediawiki/2015/7/79/Electro6.jpg">
 +
&nbsp;<img style="width: 287px; height: 289px;"
 +
alt="https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg"
 +
src="https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg"><big><span
 +
style="" lang="EN-US"></span></big><big><span
 +
style="" lang="EN-US"><span style=""></span></span></big><br>
 +
<br>
 +
<div style="text-align: left;">
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">
 +
    </span></span></span><span style=""
 +
lang="EN-US"><span style="font-weight: bold;">August:</span>
 +
All failed again. Change in methodology for the assambly standar.
 +
Restart procedures.<span style=""> </span></span></big></li>
 +
</ul>
 +
<br>
 +
</div>
 +
&nbsp; &nbsp;<img style="width: 341px; height: 169px;"
 +
alt="File:Electro9.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Electro9.jpg/800px-Electro9.jpg"
 +
srcset="/wiki/images/1/1c/Electro9.jpg 1.5x, /wiki/images/1/1c/Electro9.jpg 2x">
 +
&nbsp;<img class="shrinkToFit"
 +
alt="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg"
 +
src="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg"
 +
style="border: medium none ; vertical-align: middle; width: 335px; height: 290px;"><br>
 +
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style="font-family: Cambria;"
 +
lang="EN-US"><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">
 +
    </span></span></span></span></big><span
 +
style="" lang="EN-US"><big><span
 +
style="font-family: Cambria;"><span
 +
style="font-weight: bold;">September:</span>
 +
Finally works the KMX - RFP ligation and the plasmid.</span></big></span></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 322px; height: 243px;" alt="File:Electro8.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/4/4f/Electro8.jpg/793px-Electro8.jpg"
 +
srcset="/wiki/images/4/4f/Electro8.jpg 1.5x, /wiki/images/4/4f/Electro8.jpg 2x">
 +
<img style="width: 289px; height: 242px;"
 +
alt="File:Circulo.png"
 +
src="https://static.igem.org/mediawiki/2015/thumb/1/18/Circulo.png/714px-Circulo.png"
 +
srcset="/wiki/images/thumb/1/18/Circulo.png/1071px-Circulo.png 1.5x, /wiki/images/thumb/1/18/Circulo.png/1427px-Circulo.png 2x"><br>
 +
<br>
 
</div>
 
</div>
−
<div style="text-align: center;"><span id="result_box"
 
−
class="" lang="en"><span class="hps"></span><span
 
−
class="hps"></span></span><br>
 
 
</div>
 
</div>
−
<span id="result_box" class="" lang="en"><span
 
−
class="hps"></span></span></div>
 
−
<span id="result_box" class="" lang="en"><span
 
−
class="hps"><br>
 
−
</span></span></div>
 
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
−
<div style="text-align: center;"><br>
+
<strong style="font-family: Cambria;"></strong>
 
</div>
 
</div>
−
<div class="gwd-div-a9c8"></div>
+
<br style="font-family: Cambria;">
 +
<a style="font-family: Cambria;"
 +
href="https://2015.igem.org/Main_Page"
 +
class="gwd-a-3xpx gwd-a-36ww">
 +
<img
 +
style="border: 0px solid ; width: 150px; height: 150px; left: 989px; top: 49px;"
 +
alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
 +
class="gwd-img-kwqf"></a><a
 +
style="font-family: Cambria;"
 +
href="https://igem.org/Team_List?year=2015"
 +
class="gwd-a-3xpx gwd-a-d01x"><img
 +
src="https://static.igem.org/mediawiki/2015/thumb/8/85/Logo_Definitvo.png/654px-Logo_Definitvo.png"
 +
class="gwd-img-zpsn"></a><br
 +
style="font-family: Cambria;">
 
<br>
 
<br>
 
</body>
 
</body>
 
</html>
 
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Latest revision as of 21:00, 16 September 2015

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Notebook

This project was initiated in April of the year 2014 and in this time we have acquired new experiences like to know students from other schools and communes with our same interests, also with our same age range, which goes promoting inter-school knowledges. Also this project has served as a guide for the future, helping us to understand University life through various clases of the sience fields, as in the case of molecular biology, where it taught us the basics of this, which helped us to understand concepts like what is an plasmid?, and how use it, things which helped us to generate an idea of how to solve a problem. Then we learned various techniques from laboratories to handle the bacterias,stuff of laboratory, laboratory's machines and biobricks, essensial things for our work, and like that start own project, in this case about celiac disease.

File:Lab 800.jpg  File:Bio molecular.png

Lab (2015)
  •          January: Cultivation of Biobricks, extraction plasmidial and double digestion.

Biobricks.jpg https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg

File:Tabla.png

  •          February: Preparation of methodologies and ligation of KumaMax with RFP.  

File:Electro2jpg.png File:Methodology.jpg

  •          March: Presentation of the project to CORFO.  

File:120315 IGEM 154 copia.jpg File:Igem marzo 2015.jpg

  •          May: Samples are lost. Restart procedures.   

File:Electro3.png File:Biobricks2.jpg

  •          June: Double digestion and links which fail.  

File:Electro4.png File:Electro5.jpg

  •          July: Apply plan of ligations.

File:Electro6.jpg  https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg

  •          August: All failed again. Change in methodology for the assambly standar. Restart procedures.

   File:Electro9.jpg  https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg

  •          September: Finally works the KMX - RFP ligation and the plasmid.

File:Electro8.jpg File:Circulo.png