Difference between revisions of "Team:San Andres/Notebook"
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</head> | </head> | ||
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<tr heigth="75px"> | <tr heigth="75px"> | ||
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<a href="https://2015.igem.org/Team:San_Andres" | <a href="https://2015.igem.org/Team:San_Andres" | ||
style="color: rgb(0, 0, 0);">Home </a> </td> | style="color: rgb(0, 0, 0);">Home </a> </td> | ||
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<a href="https://2015.igem.org/Team:San_Andres/Team" | <a href="https://2015.igem.org/Team:San_Andres/Team" | ||
style="color: rgb(0, 0, 0);"> Team</a> | style="color: rgb(0, 0, 0);"> Team</a> | ||
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− | + | ||
− | + | ||
− | + | ||
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<a href="https://2015.igem.org/Team:San_Andres/Description" | <a href="https://2015.igem.org/Team:San_Andres/Description" | ||
− | style="color: rgb(0, 0, 0);"> Project </a> | + | style="color: rgb(0, 0, 0);"> Project</a> |
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<a href="https://2015.igem.org/Team:San_Andres/Parts" | <a href="https://2015.igem.org/Team:San_Andres/Parts" | ||
style="color: rgb(0, 0, 0);"> Parts</a> | style="color: rgb(0, 0, 0);"> Parts</a> | ||
</td> | </td> | ||
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<a href="https://2015.igem.org/Team:San_Andres/Modeling" | <a href="https://2015.igem.org/Team:San_Andres/Modeling" | ||
style="color: rgb(0, 0, 0);"> Modeling</a> | style="color: rgb(0, 0, 0);"> Modeling</a> | ||
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− | bgcolor="# | + | bgcolor="#ffee1b" height="30"><a |
− | + | href="https://2015.igem.org/Team:San_Andres/Results" | |
style="color: rgb(0, 0, 0);"> Results</a> | style="color: rgb(0, 0, 0);"> Results</a> | ||
</td> | </td> | ||
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+ | <a href="https://2015.igem.org/Team:San_Andres/Notebook" | ||
+ | style="color: rgb(0, 0, 0);">Notebook</a> | ||
+ | </td> | ||
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<a href="https://2015.igem.org/Team:San_Andres/Practices" | <a href="https://2015.igem.org/Team:San_Andres/Practices" | ||
− | style="color: rgb(0, 0, 0);"> Human Practices </a> | + | style="color: rgb(0, 0, 0);"> Human Practices</a> |
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<a href="https://2015.igem.org/Team:San_Andres/Design" | <a href="https://2015.igem.org/Team:San_Andres/Design" | ||
style="color: rgb(0, 0, 0);"> Future Projections</a> | style="color: rgb(0, 0, 0);"> Future Projections</a> | ||
</td> | </td> | ||
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− | <a href="https://2015.igem.org/Team:San_Andres/ | + | <a href="https://2015.igem.org/Team:San_Andres/Attributions" |
− | style="color: rgb(0, 0, 0);"> | + | style="color: rgb(0, 0, 0);"> Attributions</a> |
</td> | </td> | ||
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− | <a href="https://igem.org/ | + | onmouseout="this.bgColor='#ffee1b'" |
− | + | onmouseover="this.bgColor='#6bb402'" align="center" | |
+ | bgcolor="#ffee1b" height="30"> | ||
+ | <a href="https://2015.igem.org/Team:San_Andres/Collaborations" | ||
+ | style="color: rgb(0, 0, 0);"> Collaborations</a> | ||
</td> | </td> | ||
</tr> | </tr> | ||
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<div class="gwd-div-2ed9"></div> | <div class="gwd-div-2ed9"></div> | ||
<div class="gwd-div-7xzo gwd-a-1thu"> | <div class="gwd-div-7xzo gwd-a-1thu"> | ||
− | <h1>Notebook</h1> | + | <h1><big style="font-family: Algerian; font-weight: normal;">Notebook</big><br> |
− | <big>This project was initiated in April of the year 2014 and in | + | </h1> |
− | this time we have acquired new experiences to know students from other | + | <div style="text-align: justify; font-family: Cambria;"><big><span |
− | schools and communes with our same interests, also with our same age | + | style="color: black;">This |
− | range | + | project was initiated in April of the year 2014 and in this time we |
− | as a guide for the future, helping us to understand University life | + | have |
− | through various | + | acquired new experiences like to know students from other schools and |
− | biology, where it taught us the basics of this, which helped us to | + | communes |
− | generate an idea of how to solve a problem.</big><br> | + | with our same interests, also with our same age range, which goes |
+ | promoting | ||
+ | inter-school knowledges. Also this project has served as a guide for | ||
+ | the | ||
+ | future, helping us to understand University life through various clases | ||
+ | of the | ||
+ | sience fields, as in the case of molecular biology, where it taught us | ||
+ | the | ||
+ | basics of this, which helped us to understand concepts like what is an | ||
+ | plasmid?, and how use it, things which helped us to generate an idea of | ||
+ | how to | ||
+ | solve a problem.</span> <span style="color: black;">Then | ||
+ | we learned various techniques from laboratories to handle the | ||
+ | bacterias,stuff of laboratory, laboratory's machines and biobricks, | ||
+ | essensial | ||
+ | things for our work, and like that start own project, in this case | ||
+ | about celiac | ||
+ | disease.</span></big><span style="color: black;"><o:p></o:p></span><br> | ||
<br> | <br> | ||
+ | <span style="color: black;"></span><span | ||
+ | style="color: black;"><o:p></o:p></span></div> | ||
+ | <div style="text-align: center; font-family: Cambria;"> | ||
+ | <div style="text-align: justify;"> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
+ | style="width: 462px; height: 308px;" alt="File:Lab 800.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/0/01/Lab_800.jpg"> | ||
+ | <img style="width: 475px; height: 308px;" | ||
alt="File:Bio molecular.png" | alt="File:Bio molecular.png" | ||
− | src="https://static.igem.org/mediawiki/2015/3/39/Bio_molecular.png | + | src="https://static.igem.org/mediawiki/2015/3/39/Bio_molecular.png"> |
− | + | ||
<br> | <br> | ||
− | <div style="text-align: left;"><big> | + | <big><big><br style="font-weight: bold;"> |
− | + | </big></big> | |
− | + | <div style="text-align: left;"><big><big><span | |
+ | style="font-weight: bold;">Lab (2015)</span></big></big><br> | ||
+ | <ul> | ||
+ | <li><big><span style="font-family: Symbol;" | ||
+ | lang="EN-US"><span style=""><span | ||
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal; font-family: Cambria;"> | ||
+ | </span></span></span><span | ||
+ | style="font-family: Cambria;" lang="EN-US"><span | ||
+ | style="font-weight: bold;">January:</span> | ||
+ | Cultivation of Biobricks, extraction plasmidial and double digestion.</span></big></li> | ||
+ | <br> | ||
+ | </ul> | ||
+ | <div style="text-align: center;"><img | ||
+ | style="width: 394px; height: 234px;" alt="Biobricks.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/c/c5/Biobricks.jpg/300px-Biobricks.jpg" | ||
+ | class="thumbimage" | ||
+ | srcset="/wiki/images/c/c5/Biobricks.jpg 1.5x, /wiki/images/c/c5/Biobricks.jpg 2x"> <img | ||
+ | style="width: 513px; height: 235px;" | ||
+ | alt="https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg"><br> | ||
<br> | <br> | ||
− | </big> | + | <img style="width: 642px; height: 132px;" alt="File:Tabla.png" |
+ | src="https://static.igem.org/mediawiki/2015/e/ee/Tabla.png"><br> | ||
+ | <br> | ||
+ | </div> | ||
+ | <ul> | ||
+ | <li><big><span style=""><span | ||
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> <span | ||
+ | style="font-weight: bold;"> | ||
+ | </span></span></span><span style="" | ||
+ | lang="EN-US"><span style="font-weight: bold;">February:</span> | ||
+ | Preparation of methodologies and ligation of KumaMax with RFP.<span | ||
+ | style=""> </span></span></big></li> | ||
+ | <br> | ||
+ | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
− | style="width: | + | alt="File:Electro2jpg.png" |
− | src="https://static.igem.org/mediawiki/2015/ | + | src="https://static.igem.org/mediawiki/2015/e/e2/Electro2jpg.png" |
+ | height="374" width="278"> <img | ||
+ | style="width: 557px; height: 374px;" alt="File:Methodology.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/3/34/Methodology.jpg/800px-Methodology.jpg" | ||
+ | srcset="/wiki/images/thumb/3/34/Methodology.jpg/1200px-Methodology.jpg 1.5x, /wiki/images/3/34/Methodology.jpg 2x"><br> | ||
<br> | <br> | ||
− | <div style="text-align: | + | </div> |
− | + | <ul> | |
− | + | <li><big><span style=""><span | |
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> | ||
+ | </span></span><span style="" lang="EN-US"><span | ||
+ | style="font-weight: bold;">March:</span> | ||
+ | Presentation of the project to CORFO.<span style=""> </span></span></big></li> | ||
+ | <br> | ||
+ | </ul> | ||
+ | <div style="text-align: center;"><img | ||
+ | style="width: 374px; height: 299px;" | ||
+ | alt="File:120315 IGEM 154 copia.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/f/f3/120315_IGEM_154_copia.jpg"> | ||
+ | <img style="width: 397px; height: 298px;" | ||
+ | alt="File:Igem marzo 2015.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/8/8f/Igem_marzo_2015.jpg/800px-Igem_marzo_2015.jpg" | ||
+ | srcset="/wiki/images/8/8f/Igem_marzo_2015.jpg 1.5x, /wiki/images/8/8f/Igem_marzo_2015.jpg 2x"><br> | ||
<br> | <br> | ||
− | </big> | + | </div> |
+ | <ul> | ||
+ | <li><big><span style=""><span | ||
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> | ||
+ | </span></span><span style="" lang="EN-US"><span | ||
+ | style="font-weight: bold;">May:</span> | ||
+ | Samples are lost. Restart procedures.<span style=""> </span></span></big></li> | ||
+ | <br> | ||
+ | </ul> | ||
<div style="text-align: center;"><img | <div style="text-align: center;"><img | ||
− | alt="File: | + | style="width: 503px; height: 276px;" alt="File:Electro3.png" |
− | src="https://static.igem.org/mediawiki/2015/thumb/ | + | src="https://static.igem.org/mediawiki/2015/thumb/c/c8/Electro3.png/800px-Electro3.png" |
− | height=" | + | srcset="/wiki/images/c/c8/Electro3.png 1.5x, /wiki/images/c/c8/Electro3.png 2x"> |
+ | <img style="width: 366px; height: 274px;" | ||
+ | alt="File:Biobricks2.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/e/ee/Biobricks2.jpg"><br> | ||
+ | <br> | ||
+ | </div> | ||
+ | <ul> | ||
+ | <li><big><span style=""><span | ||
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> <span | ||
+ | style="font-weight: bold;"> | ||
+ | </span></span></span><span style="" | ||
+ | lang="EN-US"><span style="font-weight: bold;">June:</span> | ||
+ | Double digestion and links which fail.<span style=""> </span></span></big></li> | ||
+ | <br> | ||
+ | </ul> | ||
+ | <div style="text-align: center;"><img | ||
+ | alt="File:Electro4.png" | ||
+ | src="https://static.igem.org/mediawiki/2015/5/57/Electro4.png" | ||
+ | height="216" width="302"> <img | ||
+ | style="width: 417px; height: 216px;" alt="File:Electro5.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/b/bf/Electro5.jpg"><br> | ||
+ | <br> | ||
+ | </div> | ||
+ | <ul> | ||
+ | <li><big><span style=""><span | ||
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> <span | ||
+ | style="font-weight: bold;"> | ||
+ | </span></span></span><span style="" | ||
+ | lang="EN-US"><span style="font-weight: bold;">July:</span> | ||
+ | Apply plan of ligations.</span></big></li> | ||
+ | <br> | ||
+ | </ul> | ||
+ | <div style="text-align: center;"><img | ||
+ | style="width: 311px; height: 288px;" alt="File:Electro6.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/7/79/Electro6.jpg"> | ||
+ | <img style="width: 287px; height: 289px;" | ||
+ | alt="https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg"><big><span | ||
+ | style="" lang="EN-US"></span></big><big><span | ||
+ | style="" lang="EN-US"><span style=""></span></span></big><br> | ||
+ | <br> | ||
+ | <div style="text-align: left;"> | ||
+ | <ul> | ||
+ | <li><big><span style=""><span | ||
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> <span | ||
+ | style="font-weight: bold;"> | ||
+ | </span></span></span><span style="" | ||
+ | lang="EN-US"><span style="font-weight: bold;">August:</span> | ||
+ | All failed again. Change in methodology for the assambly standar. | ||
+ | Restart procedures.<span style=""> </span></span></big></li> | ||
+ | </ul> | ||
+ | <br> | ||
+ | </div> | ||
+ | <img style="width: 341px; height: 169px;" | ||
+ | alt="File:Electro9.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Electro9.jpg/800px-Electro9.jpg" | ||
+ | srcset="/wiki/images/1/1c/Electro9.jpg 1.5x, /wiki/images/1/1c/Electro9.jpg 2x"> | ||
+ | <img class="shrinkToFit" | ||
+ | alt="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" | ||
+ | style="border: medium none ; vertical-align: middle; width: 335px; height: 290px;"><br> | ||
+ | <br> | ||
+ | </div> | ||
+ | <ul> | ||
+ | <li><big><span style="font-family: Cambria;" | ||
+ | lang="EN-US"><span style=""><span | ||
+ | style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> <span | ||
+ | style="font-weight: bold;"> | ||
+ | </span></span></span></span></big><span | ||
+ | style="" lang="EN-US"><big><span | ||
+ | style="font-family: Cambria;"><span | ||
+ | style="font-weight: bold;">September:</span> | ||
+ | Finally works the KMX - RFP ligation and the plasmid.</span></big></span></li> | ||
+ | <br> | ||
+ | </ul> | ||
+ | <div style="text-align: center;"><img | ||
+ | style="width: 322px; height: 243px;" alt="File:Electro8.jpg" | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/4/4f/Electro8.jpg/793px-Electro8.jpg" | ||
+ | srcset="/wiki/images/4/4f/Electro8.jpg 1.5x, /wiki/images/4/4f/Electro8.jpg 2x"> | ||
+ | <img style="width: 289px; height: 242px;" | ||
+ | alt="File:Circulo.png" | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/1/18/Circulo.png/714px-Circulo.png" | ||
+ | srcset="/wiki/images/thumb/1/18/Circulo.png/1071px-Circulo.png 1.5x, /wiki/images/thumb/1/18/Circulo.png/1427px-Circulo.png 2x"><br> | ||
+ | <br> | ||
+ | </div> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | <strong style="font-family: Cambria;"></strong> | ||
</div> | </div> | ||
− | < | + | <br style="font-family: Cambria;"> |
− | <a href="https://igem.org/ | + | <a style="font-family: Cambria;" |
− | style="border: 0px solid ; | + | href="https://2015.igem.org/Main_Page" |
+ | class="gwd-a-3xpx gwd-a-36ww"> | ||
+ | <img | ||
+ | style="border: 0px solid ; width: 150px; height: 150px; left: 989px; top: 49px;" | ||
alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" | alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" | ||
− | class="gwd-img-kwqf"></a> | + | class="gwd-img-kwqf"></a><a |
+ | style="font-family: Cambria;" | ||
+ | href="https://igem.org/Team_List?year=2015" | ||
+ | class="gwd-a-3xpx gwd-a-d01x"><img | ||
+ | src="https://static.igem.org/mediawiki/2015/thumb/8/85/Logo_Definitvo.png/654px-Logo_Definitvo.png" | ||
+ | class="gwd-img-zpsn"></a><br | ||
+ | style="font-family: Cambria;"> | ||
<br> | <br> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 21:00, 16 September 2015
Home | Team | Project | Parts | Modeling | Results |
Notebook | Human Practices | Future Projections | Attributions | Collaborations |
Notebook
This
project was initiated in April of the year 2014 and in this time we
have
acquired new experiences like to know students from other schools and
communes
with our same interests, also with our same age range, which goes
promoting
inter-school knowledges. Also this project has served as a guide for
the
future, helping us to understand University life through various clases
of the
sience fields, as in the case of molecular biology, where it taught us
the
basics of this, which helped us to understand concepts like what is an
plasmid?, and how use it, things which helped us to generate an idea of
how to
solve a problem. Then
we learned various techniques from laboratories to handle the
bacterias,stuff of laboratory, laboratory's machines and biobricks,
essensial
things for our work, and like that start own project, in this case
about celiac
disease.
Lab (2015)
- January: Cultivation of Biobricks, extraction plasmidial and double digestion.
- February: Preparation of methodologies and ligation of KumaMax with RFP.
- March: Presentation of the project to CORFO.
- May: Samples are lost. Restart procedures.
- June: Double digestion and links which fail.
- July: Apply plan of ligations.
- August: All failed again. Change in methodology for the assambly standar. Restart procedures.
- September: Finally works the KMX - RFP ligation and the plasmid.