Difference between revisions of "Team:San Andres/Notebook"

 
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<title>exp</title>
 
<title>exp</title>
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       <a href="https://2015.igem.org/Team:San_Andres"
 
       <a href="https://2015.igem.org/Team:San_Andres"
 
  style="color: rgb(0, 0, 0);">Home </a> </td>
 
  style="color: rgb(0, 0, 0);">Home </a> </td>
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  style="color: rgb(0, 0, 0);"> Team</a>
 
  style="color: rgb(0, 0, 0);"> Team</a>
 
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      <a href="https://2015.igem.org/Team:San_Andres/Notebook"
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style="color: rgb(0, 0, 0);"> Notebook</a>
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       <a href="https://2015.igem.org/Team:San_Andres/Description"
 
       <a href="https://2015.igem.org/Team:San_Andres/Description"
−
  style="color: rgb(0, 0, 0);"> Project </a>
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  style="color: rgb(0, 0, 0);"> Project</a>
 
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       <a href="https://2015.igem.org/Team:San_Andres/Parts"
 
       <a href="https://2015.igem.org/Team:San_Andres/Parts"
 
  style="color: rgb(0, 0, 0);"> Parts</a>
 
  style="color: rgb(0, 0, 0);"> Parts</a>
 
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      <a href="https://2015.igem.org/Team:San_Andres/Modeling"
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style="color: rgb(0, 0, 0);"> Modeling</a>
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      </td>
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onmouseout="this.bgColor='#ffee1b'"
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bgcolor="#ffee1b" height="30"><a
 +
href="https://2015.igem.org/Team:San_Andres/Results"
 +
style="color: rgb(0, 0, 0);"> Results</a>
 +
      </td>
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>
 
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       <a href="https://2015.igem.org/Team:San_Andres/Modeling"
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       <a href="https://2015.igem.org/Team:San_Andres/Notebook"
−
  style="color: rgb(0, 0, 0);">Modeling</a></td>
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  style="color: rgb(0, 0, 0);">Notebook</a>
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      </td>
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      <a href="https://2015.igem.org/Team:San_Andres/Results"
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  bgcolor="#ffee1b" height="30">
−
style="color: rgb(0, 0, 0);"> Results</a></td>
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−
      <td style="border: 2px solid black;"
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       <a href="https://2015.igem.org/Team:San_Andres/Practices"
 
       <a href="https://2015.igem.org/Team:San_Andres/Practices"
−
  style="color: rgb(0, 0, 0);"> Human Practices</a></td>
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  style="color: rgb(0, 0, 0);"> Human Practices</a>
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       <a href="https://2015.igem.org/Team:San_Andres/Design"
 
       <a href="https://2015.igem.org/Team:San_Andres/Design"
−
  style="color: rgb(0, 0, 0);"> Future Projections</a></td>
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  style="color: rgb(0, 0, 0);"> Future Projections</a>
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 +
      <a href="https://2015.igem.org/Team:San_Andres/Attributions"
 +
style="color: rgb(0, 0, 0);"> Attributions</a>
 +
      </td>
 +
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       <a href="https://2015.igem.org/Team:San_Andres/Collaborations"
 
       <a href="https://2015.igem.org/Team:San_Andres/Collaborations"
−
  style="color: rgb(0, 0, 0);"> Collaborations</a></td>
+
  style="color: rgb(0, 0, 0);"> Collaborations</a>
 +
      </td>
 
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<div class="gwd-div-2ed9"></div>
 
<div class="gwd-div-2ed9"></div>
 
<div class="gwd-div-7xzo gwd-a-1thu">
 
<div class="gwd-div-7xzo gwd-a-1thu">
−
<h1>Notebook</h1>
+
<h1><big style="font-family: Algerian; font-weight: normal;">Notebook</big><br>
−
<big>This project was initiated in April of the year 2014 and in
+
</h1>
−
this time we have acquired new experiences to know students from other
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<div style="text-align: justify; font-family: Cambria;"><big><span
−
schools and communes with our same interests, also with our same age
+
style="color: black;">This
−
range thus promoting inter-school works. Also this project has served
+
project was initiated in April of the year 2014 and in this time we
−
as a guide for the future, helping us to understand University life
+
have
−
through various fields of science classes, as in the case of molecular
+
acquired new experiences like to know students from other schools and
−
biology, where it taught us the basics of this, which helped us to
+
communes
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generate an idea of how to solve a problem.</big><big>Then
+
with our same interests, also with our same age range, which goes
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we taught various techniques from laboratories to handle the
+
promoting
−
biobricks and start own project, in this case about celiac disease.</big><br>
+
inter-school knowledges. Also this project has served as a guide for
 +
the
 +
future, helping us to understand University life through various clases
 +
of the
 +
sience fields, as in the case of molecular biology, where it taught us
 +
the
 +
basics of this, which helped us to understand concepts like what is an
 +
plasmid?, and how use it, things which helped us to generate an idea of
 +
how to
 +
solve a problem.</span> <span style="color: black;">Then
 +
we learned various techniques from laboratories to handle the
 +
bacterias,stuff of laboratory, laboratory's machines and biobricks,
 +
essensial
 +
things for our work, and like that start own project, in this case
 +
about celiac
 +
disease.</span></big><span style="color: black;"><o:p></o:p></span><br>
 
<br>
 
<br>
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<div style="text-align: center;">
+
<span style="color: black;"></span><span
−
<div style="text-align: left;"><img
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style="color: black;"><o:p></o:p></span></div>
 +
<div style="text-align: center; font-family: Cambria;">
 +
<div style="text-align: justify;">
 +
<div style="text-align: center;"><img
 
  style="width: 462px; height: 308px;" alt="File:Lab 800.jpg"
 
  style="width: 462px; height: 308px;" alt="File:Lab 800.jpg"
−
  src="https://static.igem.org/mediawiki/2015/0/01/Lab_800.jpg">&nbsp;<img
+
  src="https://static.igem.org/mediawiki/2015/0/01/Lab_800.jpg">&nbsp;
−
style="width: 475px; height: 308px;"
+
<img style="width: 475px; height: 308px;"
 
  alt="File:Bio molecular.png"
 
  alt="File:Bio molecular.png"
−
  src="https://static.igem.org/mediawiki/2015/3/39/Bio_molecular.png"><br>
+
  src="https://static.igem.org/mediawiki/2015/3/39/Bio_molecular.png">
 +
<br>
 +
<big><big><br style="font-weight: bold;">
 +
</big></big>
 +
<div style="text-align: left;"><big><big><span
 +
style="font-weight: bold;">Lab (2015)</span></big></big><br>
 +
<ul>
 +
  <li><big><span style="font-family: Symbol;"
 +
lang="EN-US"><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal; font-family: Cambria;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
    </span></span></span><span
 +
style="font-family: Cambria;" lang="EN-US"><span
 +
style="font-weight: bold;">January:</span>
 +
Cultivation of Biobricks, extraction plasmidial and double digestion.</span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 394px; height: 234px;" alt="Biobricks.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/c/c5/Biobricks.jpg/300px-Biobricks.jpg"
 +
class="thumbimage"
 +
srcset="/wiki/images/c/c5/Biobricks.jpg 1.5x, /wiki/images/c/c5/Biobricks.jpg 2x">&nbsp;<img
 +
style="width: 513px; height: 235px;"
 +
alt="https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg"><br>
 +
<br>
 +
<img style="width: 642px; height: 132px;" alt="File:Tabla.png"
 +
src="https://static.igem.org/mediawiki/2015/e/ee/Tabla.png"><br>
 
<br>
 
<br>
 
</div>
 
</div>
−
<div style="text-align: left;"><big></big>
+
<ul>
−
<div style="text-align: center;">
+
  <li><big><span style=""><span
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<div style="text-align: left;"><big>To be able to
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style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
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raise funds have performed different activities, including: musical
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style="font-weight: bold;">
−
events, toy sales, yard sales, zumba, raffles, etc. We also have the
+
    </span></span></span><span style=""
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support of the Mayor in our commun, who spoke at a breakfast on the
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lang="EN-US"><span style="font-weight: bold;">February:</span>
−
importance of the project.<br>
+
Preparation of methodologies and ligation of KumaMax with RFP.<span
 +
style="">&nbsp;&nbsp;</span></span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
alt="File:Electro2jpg.png"
 +
src="https://static.igem.org/mediawiki/2015/e/e2/Electro2jpg.png"
 +
height="374" width="278"> <img
 +
style="width: 557px; height: 374px;" alt="File:Methodology.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/3/34/Methodology.jpg/800px-Methodology.jpg"
 +
srcset="/wiki/images/thumb/3/34/Methodology.jpg/1200px-Methodology.jpg 1.5x, /wiki/images/3/34/Methodology.jpg 2x"><br>
 
<br>
 
<br>
−
</big></div>
+
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−
<div style="text-align: left;"><big></big>
+
<ul>
−
<div style="text-align: left;"><img
+
  <li><big><span style=""><span
−
  style="width: 496px; height: 569px;" alt="File:Ventas.jpg"
+
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
−
  src="https://static.igem.org/mediawiki/2015/thumb/d/dd/Ventas.jpg/521px-Ventas.jpg">&nbsp;<img
+
    </span></span><span style="" lang="EN-US"><span
−
  style="width: 427px; height: 568px;" alt="File:Alcalde.jpg"
+
style="font-weight: bold;">March:</span>
−
  src="https://static.igem.org/mediawiki/2015/thumb/d/de/Alcalde.jpg/450px-Alcalde.jpg"></div>
+
Presentation of the project to CORFO.<span style="">&nbsp;&nbsp;</span></span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 374px; height: 299px;"
 +
alt="File:120315 IGEM 154 copia.jpg"
 +
src="https://static.igem.org/mediawiki/2015/f/f3/120315_IGEM_154_copia.jpg">
 +
<img style="width: 397px; height: 298px;"
 +
alt="File:Igem marzo 2015.jpg"
 +
src="https://static.igem.org/mediawiki/2015/thumb/8/8f/Igem_marzo_2015.jpg/800px-Igem_marzo_2015.jpg"
 +
srcset="/wiki/images/8/8f/Igem_marzo_2015.jpg 1.5x, /wiki/images/8/8f/Igem_marzo_2015.jpg 2x"><br>
 +
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
    </span></span><span style="" lang="EN-US"><span
 +
style="font-weight: bold;">May:</span>
 +
Samples are lost. Restart procedures.<span style="">&nbsp;&nbsp;&nbsp;</span></span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 503px; height: 276px;" alt="File:Electro3.png"
 +
src="https://static.igem.org/mediawiki/2015/thumb/c/c8/Electro3.png/800px-Electro3.png"
 +
srcset="/wiki/images/c/c8/Electro3.png 1.5x, /wiki/images/c/c8/Electro3.png 2x">
 +
<img style="width: 366px; height: 274px;"
 +
alt="File:Biobricks2.jpg"
 +
src="https://static.igem.org/mediawiki/2015/e/ee/Biobricks2.jpg"><br>
 +
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">&nbsp;
 +
    </span></span></span><span style=""
 +
lang="EN-US"><span style="font-weight: bold;">June:</span>
 +
Double digestion and links which fail.<span style="">&nbsp;&nbsp;</span></span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
alt="File:Electro4.png"
 +
src="https://static.igem.org/mediawiki/2015/5/57/Electro4.png"
 +
height="216" width="302"> <img
 +
  style="width: 417px; height: 216px;" alt="File:Electro5.jpg"
 +
src="https://static.igem.org/mediawiki/2015/b/bf/Electro5.jpg"><br>
 +
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">&nbsp;&nbsp;
 +
    </span></span></span><span style=""
 +
lang="EN-US"><span style="font-weight: bold;">July:</span>
 +
Apply plan of ligations.</span></big></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
style="width: 311px; height: 288px;" alt="File:Electro6.jpg"
 +
src="https://static.igem.org/mediawiki/2015/7/79/Electro6.jpg">
 +
&nbsp;<img style="width: 287px; height: 289px;"
 +
alt="https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg"
 +
src="https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg"><big><span
 +
style="" lang="EN-US"></span></big><big><span
 +
style="" lang="EN-US"><span style=""></span></span></big><br>
 +
<br>
 +
<div style="text-align: left;">
 +
<ul>
 +
  <li><big><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">
 +
    </span></span></span><span style=""
 +
lang="EN-US"><span style="font-weight: bold;">August:</span>
 +
All failed again. Change in methodology for the assambly standar.
 +
Restart procedures.<span style=""> </span></span></big></li>
 +
</ul>
 +
<br>
 +
</div>
 +
&nbsp; &nbsp;<img style="width: 341px; height: 169px;"
 +
alt="File:Electro9.jpg"
 +
  src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Electro9.jpg/800px-Electro9.jpg"
 +
srcset="/wiki/images/1/1c/Electro9.jpg 1.5x, /wiki/images/1/1c/Electro9.jpg 2x">
 +
&nbsp;<img class="shrinkToFit"
 +
alt="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg"
 +
src="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg"
 +
style="border: medium none ; vertical-align: middle; width: 335px; height: 290px;"><br>
 +
<br>
 +
</div>
 +
<ul>
 +
  <li><big><span style="font-family: Cambria;"
 +
lang="EN-US"><span style=""><span
 +
style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span
 +
style="font-weight: bold;">
 +
    </span></span></span></span></big><span
 +
style="" lang="EN-US"><big><span
 +
style="font-family: Cambria;"><span
 +
style="font-weight: bold;">September:</span>
 +
Finally works the KMX - RFP ligation and the plasmid.</span></big></span></li>
 +
  <br>
 +
</ul>
 +
<div style="text-align: center;"><img
 +
  style="width: 322px; height: 243px;" alt="File:Electro8.jpg"
 +
  src="https://static.igem.org/mediawiki/2015/thumb/4/4f/Electro8.jpg/793px-Electro8.jpg"
 +
srcset="/wiki/images/4/4f/Electro8.jpg 1.5x, /wiki/images/4/4f/Electro8.jpg 2x">
 +
<img style="width: 289px; height: 242px;"
 +
alt="File:Circulo.png"
 +
src="https://static.igem.org/mediawiki/2015/thumb/1/18/Circulo.png/714px-Circulo.png"
 +
srcset="/wiki/images/thumb/1/18/Circulo.png/1071px-Circulo.png 1.5x, /wiki/images/thumb/1/18/Circulo.png/1427px-Circulo.png 2x"><br>
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Latest revision as of 21:00, 16 September 2015

<endnote><head> <title><endnote><head> <meta> <title> wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Home Team Project Parts Modeling Results
Notebook Human Practices Future Projections Attributions Collaborations

Notebook

This project was initiated in April of the year 2014 and in this time we have acquired new experiences like to know students from other schools and communes with our same interests, also with our same age range, which goes promoting inter-school knowledges. Also this project has served as a guide for the future, helping us to understand University life through various clases of the sience fields, as in the case of molecular biology, where it taught us the basics of this, which helped us to understand concepts like what is an plasmid?, and how use it, things which helped us to generate an idea of how to solve a problem. Then we learned various techniques from laboratories to handle the bacterias,stuff of laboratory, laboratory's machines and biobricks, essensial things for our work, and like that start own project, in this case about celiac disease.

File:Lab 800.jpg  File:Bio molecular.png

Lab (2015)
  •          January: Cultivation of Biobricks, extraction plasmidial and double digestion.

Biobricks.jpg https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg

File:Tabla.png

  •          February: Preparation of methodologies and ligation of KumaMax with RFP.  

File:Electro2jpg.png File:Methodology.jpg

  •          March: Presentation of the project to CORFO.  

File:120315 IGEM 154 copia.jpg File:Igem marzo 2015.jpg

  •          May: Samples are lost. Restart procedures.   

File:Electro3.png File:Biobricks2.jpg

  •          June: Double digestion and links which fail.  

File:Electro4.png File:Electro5.jpg

  •          July: Apply plan of ligations.

File:Electro6.jpg  https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg

  •          August: All failed again. Change in methodology for the assambly standar. Restart procedures.

   File:Electro9.jpg  https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg

  •          September: Finally works the KMX - RFP ligation and the plasmid.

File:Electro8.jpg File:Circulo.png