Difference between revisions of "Team:BroadRun-NorthernVA/Parts"

 
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<h2> Part Documentation</h2>
 
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
  
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<font size="4">
  
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<div class="highlightBox">
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<table width=90% align=center class="parts">
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<h4>Note</h4>
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<tr><br><br><br>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<td><h3><b>PART NAME</b></h3></td>
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</div>
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<td><h3><b>PART NUMBER</b></h3></td>
  
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</tr>
  
  
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<h4>Adding parts to the registry</h4>
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<tr>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<td>1. Promoterless alpha amylase with native secretion</td>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<td><a href="http://parts.igem.org/Part:BBa_K1871000 "> BBa_K1871000 </a></td>
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</tr>
  
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<tr>
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<td>2. Alpha amylase with native secretion</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1871001 "> BBa_K1871001 </a></td>
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</tr>
  
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<tr>
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<p>The information needed to initially create a part on the Registry is:</p>
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<td>3. Alpha amylase with mating factor alpha secretion</td>
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<ul>
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<td><a href="http://parts.igem.org/Part:BBa_K1871002 "> BBa_K1871002 </a></td>
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<li>Part Name</li>
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</tr>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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</table>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<br></br><br></br>
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<p class="pageheading"> <font size="4"><b>Composite Part 1: BBa_K1871000 </b>
  
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<li><span>Promoterless </span></li>
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<li><span>Kozak sequence (Part BBa_K165002) </span></li>
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<li><span> Native secretion sequence, from Bacillus amyloliquefaciens</span></li>
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<li><span> Alpha amylase coding sequence from Bacillus amyloliquefaciens</span></li>
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<li><span> ADH1 Terminator (Part BBa_K392003)</span></li>
  
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<br></br>
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<p class="pageheading"><font size="4"> <b>Composite Part 2: BBa_K1871001  </b>
  
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<li><span>cyc promoter (Part BBa_K105027) </span></li>
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<li><span>Kozak sequence (Part BBa_K165002) </span></li>
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<li><span> Native secretion sequence, from Bacillus amyloliquefaciens</span></li>
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<li><span> Alpha amylase coding sequence from Bacillus amyloliquefaciens</span></li>
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<li><span> ADH1 Terminator (Part BBa_K392003)</span></li>
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<br></br>
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<p class="pageheading"> <font size="4"><b>Composite Part 3: BBa_K1871002 </b>
  
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<li><span> cyc promoter (Part BBa_K105027) </span></li>
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<li><span>Kozak sequence (Part BBa_K165002) </span></li>
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<li><span> Native secretion sequence, from Bacillus amyloliquefaciens</span></li>
 +
<li><span> Alpha amylase coding sequence from Bacillus amyloliquefaciens</span></li>
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<li><span> ADH1 Terminator (Part BBa_K392003)</span></li>
  
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<br></br>
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<h4>Inspiration</h4>
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<font size="4">
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<b>Parts Description:</b>
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<br></br>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<b>ADH1 Terminator-</b>
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<ul>
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This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<br></br>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<b>Kozak Sequence-</b>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.  
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</ul>
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<br></br>
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<b> cyc minimal promoter-</b>
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This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.
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<br></br>
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<h4>Part Table </h4>
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<b>Mating Factor Alpha Secretion Sequence-</b>
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</html>
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This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.
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<groupparts>iGEM015 Example</groupparts>
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<br></br>
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<html>
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<b>Alpha Amylase-</b>
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Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.
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<br></br>
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<b>Native Alpha Amylase Secretion Sequence</b>
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A signal sequence which directs the protein to be secreted.
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Latest revision as of 02:26, 17 September 2015

{{BroadRun-NorthernVA}}



>



PART NAME

PART NUMBER
1. Promoterless alpha amylase with native secretion BBa_K1871000
2. Alpha amylase with native secretion BBa_K1871001
3. Alpha amylase with mating factor alpha secretion BBa_K1871002




Composite Part 1: BBa_K1871000

  • Promoterless
  • Kozak sequence (Part BBa_K165002)
  • Native secretion sequence, from Bacillus amyloliquefaciens
  • Alpha amylase coding sequence from Bacillus amyloliquefaciens
  • ADH1 Terminator (Part BBa_K392003)


    • Composite Part 2: BBa_K1871001

  • cyc promoter (Part BBa_K105027)
  • Kozak sequence (Part BBa_K165002)
  • Native secretion sequence, from Bacillus amyloliquefaciens
  • Alpha amylase coding sequence from Bacillus amyloliquefaciens
  • ADH1 Terminator (Part BBa_K392003)


    • Composite Part 3: BBa_K1871002

  • cyc promoter (Part BBa_K105027)
  • Kozak sequence (Part BBa_K165002)
  • Native secretion sequence, from Bacillus amyloliquefaciens
  • Alpha amylase coding sequence from Bacillus amyloliquefaciens
  • ADH1 Terminator (Part BBa_K392003)


    • Parts Description:

    ADH1 Terminator- This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.

    Kozak Sequence- This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.

    cyc minimal promoter- This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.

    Mating Factor Alpha Secretion Sequence- This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.

    Alpha Amylase- Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

    Native Alpha Amylase Secretion Sequence A signal sequence which directs the protein to be secreted.