Difference between revisions of "Team:BroadRun-NorthernVA/Safety"

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{{BroadRun-NorthernVA}}
 
{{BroadRun-NorthernVA}}
 
<html>
 
<html>
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<h2>Safety in iGEM</h2>
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<!-- Start of CSS-->
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<style type="text/css">
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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/* PAGE LAYOUT */
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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/* Change Background color*/
 +
body {FFCC66
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background-color: #FFD699
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}
  
 +
/* Creates a container that will wrap all of the content inside your wiki pages. */
 +
#mainContainer { 
 +
width: 978px;
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overflow:hidden;
 +
float:left;
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margin-left:20px;
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margin-bottom: 10px;
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background-color: #FFE680;
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border-bottom: 814px solid #FFE680;
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border-right: 2px solid #000000;
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border-left: 2px solid #000000;
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border-top: 5px solid #000000;
 +
font-family: "Helvetica", Helvetica, sans-serif;
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}
  
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<h4>Safe Project Design</h4>
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/* Creates the container for the menu */
 +
#menuContainer  {
 +
float:left;
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width: 134px;
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padding: 20px 0px;
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border-top: 14px solid #7A0000;
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background-color: #7A0000;
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}
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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/* Creates the container for the content */
 +
#contentContainer {
 +
padding-top:20px;
 +
padding-right:10px;
 +
margin-bottom: 20px;
 +
width: 814px;
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padding-left: 20px;
 +
float: left;
 +
background-color: #FFE680;
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border-top: 14px solid #7A0000;
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font-family: "Georgia", Helvetica, sans-serif;
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}
  
 +
/*Set up height place holder for the banner*/
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#bannerContainer {
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height:200px;
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margin:auto;
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text-align:center;
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color: #FFCC66;
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}
 +
 +
/*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */
 +
 +
/*Change the styling of text for everything inside main container*/
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#mainContainer p {
 +
font-size: 13px;
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color: #000000;
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}
 +
 +
/*This changes the color and font family */
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#contentContainer h1, h2, h3, h4, h5, h6 {
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color: #000000;
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border-bottom: none;
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font-weight: bold;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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margin-top:10px;
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}
 +
 +
/*Style of the links - links are different inside the menu */
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#contentContainer a {
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font-weight: bold;
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color: #000000;
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}
 +
 +
  /* Styling links on hover- links are different inside the menu */
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#contentContainer a:hover {
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color: #FFFFFF
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}
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 +
/*Change the styling of tables */
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        #contentContainer table {
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    border: 1px solid #000000;
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    border-collapse: collapse;
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    width: 90%
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    margin: auto;
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    margin-bottom: 15px;
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    margin-top: 15px;
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    margin-right: 10px;
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    margin-left: 10px;
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    }
 +
 +
/*Change the styling of table cells*/
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    #contentContainer  td {
 +
    padding: 10px;
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    border: 1px solid #00000;
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    border-collapse: collapse;
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    vertical-align: text-top;
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    }
 +
 +
/*Change the styling of table headers */
 +
    #contentContainer th {
 +
    background-color: #000000;
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    padding: 10px;
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    border: 1px solid #565656;
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    border-collapse: collapse;
 +
    vertical-align: text-top;
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    }
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 +
 +
 +
/*MENU STYLING */
 +
 +
/*Styling for the links in the menu */
 +
#menuContainer a {
 +
color: #FFFFFF;
 +
text-decoration:none;
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font-weight: bold;
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}
 +
 +
/* Sets the style for lists inside menuContainer  */
 +
#menuContainer ul {
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list-style: none;
 +
margin-left:0px;
 +
}
 +
 +
/*Styles the list items to become menu buttons */
 +
#menuContainer ul li {
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text-align: center;
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display: block;
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width: 100%;
 +
height:30px;
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padding-top:10px;
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}
 +
 +
/*For the menu buttons, changes the color when hovering*/
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#menuContainer li:hover {
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color:  #FFFFFF;
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background-color: #000000;
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}
 +
 +
/*Submenus are not displayed as default*/
 +
#menuContainer li ul {
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display: none;
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padding-top:15px;
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margin-left: -19px;
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}
 +
 +
/*Submenus are displayed when hovering the menu button */
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#menuContainer li:hover ul {
 +
/*display: inline-block; */
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display: block;
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position: absolute;
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float:right;
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margin-left: 134px;
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margin-top:-42px;
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}
 +
 +
/*Style the submenu buttons*/
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#menuContainer li ul li{
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background-color: #800000;
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padding-left:20px;
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padding-right:20px;
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height:30px;
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padding-top:10px;
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margin-top:-2px;
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color: #FFFFFF;
 +
width: 150px;
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}
 +
 +
/*CLASSES */
 +
 +
/*Clear class for all the pages, adds spacing too*/
 +
.clear{
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clear:both;
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height: 10px;
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}
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 +
 +
/* highlight box for special messages */
 +
    .highlightBox {
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        width:500px;
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        margin:auto;
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        background-color: #7A0000;
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margin-bottom: 15px;
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margin-top: 15px;
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padding: 15px;
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padding-top: 5px;
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    }
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 +
 +
</style>
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<!-- End of CSS -->
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<font size="4">
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<h2>Safety in iGEM</h2>
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 +
<p><font size="4">To insure the safety of our team and the world outside of our operations, we took many steps to negate the possibility of the biological destruction of humanity. Here are the steps we used:</p>
 +
<br></br>
 +
<p><b><font size="4">Planning:</b>
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<font size="4">Our journey began within the walls of our cozy laboratory, our team spent hours planning the safest approach to our experiment. We decided to use a harmless strain of yeast to conduct our experiments on amylase.
 +
</p>
 +
<br></br>
 +
<p><b><font size="4">Lab Safety:</b>
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<font size="4">To negate the possibility of any physical injuries, proper lab conduct was followed at all times.  These procedures included:
 +
</p>
 
<ul>
 
<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<font size="4">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li> Wearing gloves and goggles
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Disinfecting the workspace
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<li>Following chemical safety procedures
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</ul>
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<li>Maintaining a neat and well organized work environment
 +
<li>Using bleach to sterilize unused cultures
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<li>Keeping food and drink out of the lab
 +
<br></br>
  
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<h4>Safe Lab Work</h4>
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<img src="https://static.igem.org/mediawiki/2015/9/90/BRSafety.jpeg" alt="image" style="width:260.8px;height:225px;">
 +
<br></br>
 +
<font size="4">
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<p><b><font size="4">Product Safety</b>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<p><font size="4">Before we allowed our product to leave the lab we carefully tested for any adverse effects it could have on the environment. </p>
 +
<p><font size="4">There are several options to minimize risks to the environment: </p>
 +
<ul>
 +
<font size="4">
 +
<li>Incorporate a kill switch after a certain number of generations, or activated by a certain compound, so that the genetically modified yeast cells will not have a chance adversely affect the environment in the event the cells escape the water system/lab
 +
</li>
 +
<li>Minimize the risk that our genetically engineered yeast will escape into the environment by isolating the secreted amylase from the microbial culture, and only using the enzyme to treat the water.  The yeast itself, will never leave the lab.
 +
</li>
 +
</font>
 +
</ul>
  
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<h4>Safe Shipment</h4>
 
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
  
  
 
</div>
 
</div>
 
</html>
 
</html>

Latest revision as of 03:09, 17 September 2015

{{BroadRun-NorthernVA}}



>

Safety in iGEM

To insure the safety of our team and the world outside of our operations, we took many steps to negate the possibility of the biological destruction of humanity. Here are the steps we used:



Planning: Our journey began within the walls of our cozy laboratory, our team spent hours planning the safest approach to our experiment. We decided to use a harmless strain of yeast to conduct our experiments on amylase.



Lab Safety: To negate the possibility of any physical injuries, proper lab conduct was followed at all times. These procedures included:

  • Wearing gloves and goggles
  • Disinfecting the workspace
  • Following chemical safety procedures
  • Maintaining a neat and well organized work environment
  • Using bleach to sterilize unused cultures
  • Keeping food and drink out of the lab

    image

    Product Safety

    Before we allowed our product to leave the lab we carefully tested for any adverse effects it could have on the environment.

    There are several options to minimize risks to the environment:

    • Incorporate a kill switch after a certain number of generations, or activated by a certain compound, so that the genetically modified yeast cells will not have a chance adversely affect the environment in the event the cells escape the water system/lab
    • Minimize the risk that our genetically engineered yeast will escape into the environment by isolating the secreted amylase from the microbial culture, and only using the enzyme to treat the water. The yeast itself, will never leave the lab.