Difference between revisions of "Team:ETH Zurich/Basic Part"

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<p>In this section we present our basic parts. We expressed <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">human Annexin V</a> in <i>Escherichia coli</i> and then made a fusion protein with <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">Annexin V and the Ice Nucleation Protein (INP)</a>. The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine. </p>
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<p>In this section we present our basic parts. Our part is LldR, a protein required to regulate the lldR promoter. The <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Characterization_of_the_LldR_promoter">expression of LldR</a> was necessary to characterize the natural and our <a href="https://2015.igem.org/Team:ETH_Zurich/Part_Collection">designed lldR promoters</a>. We also give here an operon with LldP and LldR. LldP is an L-lactate permease which transports L-lactate inside the cell by proton motion force. It increases the levels of lactate inside the cell and it changes the behaviour of our <a href="https://2015.igem.org/Team:ETH_Zurich/Part_Collection">designed promoters</a>. We believe that our system combining LldR, LldP and P<sub>lldR</sub> can be <b>used by future iGEM teams</b> as means of lactate detection.</p>
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<p>Our other part is LldR, a protein required to regulate the lldR promoter. The <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Characterization_of_the_LldR_promoter">expression of LldR</a> was necessary to characterize the natural and our <a href="https://2015.igem.org/Team:ETH_Zurich/Part_Collection">designed lldR promoters</a>. </p>
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Revision as of 08:48, 17 September 2015

"What I cannot create I do not understand."
- Richard Feynmann

Basic Parts

In this section we present our basic parts. Our part is LldR, a protein required to regulate the lldR promoter. The expression of LldR was necessary to characterize the natural and our designed lldR promoters. We also give here an operon with LldP and LldR. LldP is an L-lactate permease which transports L-lactate inside the cell by proton motion force. It increases the levels of lactate inside the cell and it changes the behaviour of our designed promoters. We believe that our system combining LldR, LldP and PlldR can be used by future iGEM teams as means of lactate detection.

Part

Content

Registry number
Annexin V Promoter J23114-RBS B0034- Annexin V optimized for Escherichia coli BBa_K1847000
INP-Annexin V Promoter J23114-RBS B0034- INP-Annexin V fusion protein BBa_K1847015
LldR lldR BBa_K1847001
LldP-LldR lldP-lldR BBa_K1847016

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