Difference between revisions of "Team:UFSCar-Brasil/results.html"
Celio.dias (Talk | contribs) |
Celio.dias (Talk | contribs) |
||
Line 12: | Line 12: | ||
Results | Results | ||
</h1> | </h1> | ||
− | <h2 class="ui teal header"> | + | <h2 class="ui teal header">What did we observe?</h2> |
</div> | </div> | ||
<div class="spacer"></div> | <div class="spacer"></div> | ||
Line 19: | Line 19: | ||
<div class="ui vertical stripe segment container"> | <div class="ui vertical stripe segment container"> | ||
− | |||
<h3 class="ui header" id="overview">Plasmolysis</h3> | <h3 class="ui header" id="overview">Plasmolysis</h3> | ||
<p> Main objective of this section is to verify the viability of Escherichia coli cells under treatments with polyethylene glycol (PEG 6000) for different time lapses, and the retaken of metabolism after plasmolysis. To this, we submited this organism to different concentrations of PEG 6000 and have assessed its growth hability. Assays of plasmolysis induction were performed using Escherichia coli K-12 strain Dh5α cells obtained from a culture in lysogeny broth until an optical density at 600 nm of ~ 1.5-2.0, finally cells were harvested by centrifugation and washed with sterile 0.9% saline. Cells were resuspended in plasmolysis media (2% (vo./vol.) glycerol, 50 mM sodium acetate, 10 mM zinc chloride) containing various different concentrations of PEG 6000 (5, 10, 15, 20, 25 and 30% wt/vol). Proportion of cells in the final solution was 0.1% wt/vol. All media and their corresponding sterile controls were kept in triplicate at room temperature.</p> | <p> Main objective of this section is to verify the viability of Escherichia coli cells under treatments with polyethylene glycol (PEG 6000) for different time lapses, and the retaken of metabolism after plasmolysis. To this, we submited this organism to different concentrations of PEG 6000 and have assessed its growth hability. Assays of plasmolysis induction were performed using Escherichia coli K-12 strain Dh5α cells obtained from a culture in lysogeny broth until an optical density at 600 nm of ~ 1.5-2.0, finally cells were harvested by centrifugation and washed with sterile 0.9% saline. Cells were resuspended in plasmolysis media (2% (vo./vol.) glycerol, 50 mM sodium acetate, 10 mM zinc chloride) containing various different concentrations of PEG 6000 (5, 10, 15, 20, 25 and 30% wt/vol). Proportion of cells in the final solution was 0.1% wt/vol. All media and their corresponding sterile controls were kept in triplicate at room temperature.</p> | ||
Line 27: | Line 26: | ||
<h5 class="ui center aligned header"><b>Figure 1</b>: Experimental plot of absorbance versus time in different PEG 6000 concentration.</h5> | <h5 class="ui center aligned header"><b>Figure 1</b>: Experimental plot of absorbance versus time in different PEG 6000 concentration.</h5> | ||
+ | |||
+ | |||
</div> | </div> |
Revision as of 13:44, 17 September 2015
Results
What did we observe?
Plasmolysis
Main objective of this section is to verify the viability of Escherichia coli cells under treatments with polyethylene glycol (PEG 6000) for different time lapses, and the retaken of metabolism after plasmolysis. To this, we submited this organism to different concentrations of PEG 6000 and have assessed its growth hability. Assays of plasmolysis induction were performed using Escherichia coli K-12 strain Dh5α cells obtained from a culture in lysogeny broth until an optical density at 600 nm of ~ 1.5-2.0, finally cells were harvested by centrifugation and washed with sterile 0.9% saline. Cells were resuspended in plasmolysis media (2% (vo./vol.) glycerol, 50 mM sodium acetate, 10 mM zinc chloride) containing various different concentrations of PEG 6000 (5, 10, 15, 20, 25 and 30% wt/vol). Proportion of cells in the final solution was 0.1% wt/vol. All media and their corresponding sterile controls were kept in triplicate at room temperature.
Primary spectrophotometry analysis, by checking optical density at 600 nm of all triplicates with different concentrations of PEG 6000, as well as the control medium without PEG showed cell growth was inhibited with increasing PEG 6000 concentrations used for stress condition induction (Figure 1).
![](https://2015.igem.org/File:UFSCariGEM2015_Figure1_plasmolysis.jpg)
Figure 1: Experimental plot of absorbance versus time in different PEG 6000 concentration.
Our amazing sponsors!
![](https://static.igem.org/mediawiki/2015/2/24/UFSCar-Brasil_logos1.png)