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| </html>{{UNITN-Trento/globalIncluder}}<html> | | </html>{{UNITN-Trento/globalIncluder}}<html> |
− | <script src="https://2015.igem.org/Template:UNITN-Trento/JSModernizer?action=raw&ctype=text/javascript"></script>
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− | <script src="https://2015.igem.org/Template:UNITN-Trento/JSscrollTo?action=raw&ctype=text/javascript"></script>
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− | <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:UNITN-Trento/CSSanimate?action=raw&ctype=text/css"></link> | + | <script src="https://2015.igem.org/Template:UNITN-Trento/JSnotebookFunctions?action=raw&ctype=text/javascript"></script> |
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− | <script src="https://2015.igem.org/Template:UNITN-Trento/JSWow?action=raw&ctype=text/javascript"></script> | + | <link rel="stylesheet" type="text/css" href="http://www.startrekfederation.it/transfer_area/timeline.css?action=raw&ctype=text/css" /> |
− | | + | <style>[class^="flaticon-"]:before, [class*=" flaticon-"]:before,[class^="flaticon-"]:after, [class*=" flaticon-"]:after{font-family:Flaticon;font-size:40px;font-style:normal;margin-left:3px}</style> |
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| <header id="header"></html> | | <header id="header"></html> |
| {{UNITN-Trento/mainMenu}} | | {{UNITN-Trento/mainMenu}} |
− | <html><script>jQuery('#menulin_more, #menulin_more_safety').addClass('current');</script></header> | + | <html><script> |
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| + | jQuery('#menulin_interlab').addClass('current'); |
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| + | </script></header> |
| <!-- Main --> | | <!-- Main --> |
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− | <section id="cta" data-stellar-background-ratio="0.4" style="background-image:url('https://static.igem.org/mediawiki/2015/6/65/Unitn_pics_cta_safety.jpg');"> | + | <section id="cta" style="background-image:url('https://static.igem.org/mediawiki/2015/0/04/Unitn_pics_slider_5.jpg');"> |
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| <header> | | <header> |
− | <h2><strong> Safety </strong></h2> | + | <h2><strong>InterLab Measurement Study</strong></h2> |
− | <p> Work hard, work safe </p> | + | <p>What happens when GFP meets different promoters? Are they all the same? Here is what we found out!</p> |
| </header> | | </header> |
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| </section> | | </section> |
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| + | <article id="main"> |
− | <!-- BEGIN iGEM_Content_Section -->
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− | <article id="main"> | + | <a class="anchor-off" name="interlab_intro" id="interlab_intro"></a> |
− | <section class="wrapper style1 container special wow fadeInLeft" style="margin-top:3em;">
| + | <section class="wrapper style4 container" style="margin-top:1em;"> |
− | <div class="row">
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− | <div class="4u 12u(narrower)">
| + | <div class="content"> |
− | | + | <section> |
− | <section>
| + | <header> |
− | <header>
| + | <h3 class="wow fadeInDown">The Interlab Measurement Study</h3> |
− | <div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria"></i></span></div>
| + | </header> |
− | </header>
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− | <p>We have reviewed our organisms and parts against the <a class="i_linker" href="https://2015.igem.org/Safety/WhiteList" target="_blank">White List</a>. All the parts and bacterial strains that we used are in the <span class="i_enph">Risk Group 1</span></p>
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− | </section>
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− | | + | <div class="row"> |
− | </div>
| + | <div class="4u 12u(narrower)"> |
− | <div class="4u 12u(narrower)">
| + | <div href="#" class="wow zoomIn rotate-box square-icon"> |
− | | + | <span class="rotate-box-icon tals"><i class="faa flaticon-up151"></i></span> |
− | <section>
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− | <header>
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− | <div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-checkmark11"></i></span></div>
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− | </header>
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− | <p>We have submitted to iGEM headquarters <span class="i_enph">“About Our Lab”</span> and <span class="i_enph">“About Our Project”</span> questionnaires by the June 26<sup>th</sup> deadline</p>
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− | </section> | + | |
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− | </div>
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− | <div class="4u 12u(narrower)">
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− | <section>
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− | <header>
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− | <div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-verified7"></i></span></div> | + | |
− | </header>
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− | <p>We have submitted to iGEM headquarters the <span class="i_enph">“Final Safety Form”</span> by the August 28<sup>th</sup> deadline</p>
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− | </section>
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− | </div>
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− | </div>
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− | </section>
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− | <!-- One -->
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− | <section class="wrapper style4 container wow fadeInRight" style="margin-top:1em;">
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− | <div class="content">
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− | <section>
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− | <header>
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− | <h3>About Safety</h3>
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− | </header>
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− |
| + | |
− | <p>We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:</p>
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− | <ul class="checklist">
| + | |
− | <li>General considerations on risks and prevention</li>
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− | <li>Electrical Hazard</li>
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− | <li>Chemical Hazard</li>
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− | <li>Biological Hazard</li>
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− | <li>Compressed gasses and cryogenic liquids</li>
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− | </ul>
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− | <p>We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.</p>
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− | </section>
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− | </div>
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− | </section>
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− | <!-- Content -->
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− | <div class="content">
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− | <section>
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− | <!--<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>-->
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− | <header>
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− | <h3>Safe Project Design</h3>
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− | </header>
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− | <!-- TODO -->
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− | <div class="row">
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− | <div class="6u 12u(narrower)">
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− | <section>
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− | <header>
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− | <p class="question">Who will use your product? What opinions do these people have about your project?</p>
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− | </header>
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− | <p>Answer</p>
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− | </section>
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− | </div>
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− | <div class="6u 12u(narrower)">
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− | <section>
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− | <header>
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− | <p class="question">Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?</p>
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− | </header>
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− | <p>Answer</p>
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− | </section>
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− | </div>
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− | </div>
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− |
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− | <div class="row">
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− | <div class="6u 12u(narrower)">
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− | <section>
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− | <header>
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− | <p class="question">If your product is successful, who will receive benefits and who will be harmed?</p>
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− | </header>
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− | <p>Answer</p>
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− | </section>
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− | </div>
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− | <div class="6u 12u(narrower)">
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− | <section>
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− | <header>
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− | <p class="question">What happens when it's all used up? Will it be sterilized, discarded, or recycled?</p>
| + | |
− | </header>
| + | |
− | <p>Answer</p>
| + | |
− | </section>
| + | |
− | </div>
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| </div> | | </div> |
− |
| + | </div> |
− | <div class="row">
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− | <div class="6u 12u(narrower)">
| + | |
− | <section>
| + | |
− | <header>
| + | </section> |
− | <p class="question">Is it safer, cheaper, or better than other technologies that do the same thing?</p>
| + | </div> |
− | </header>
| + | </section> |
− | <p>Answer</p>
| + | |
− | </section>
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− | </div>
| + | |
− | <div class="6u 12u(narrower)">
| + | |
− | <section>
| + | |
− | <header>
| + | |
− | <p class="question">__</p>
| + | |
− | </header>
| + | |
− | <p>Answer</p>
| + | |
− | </section>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </section> | + | |
− | </div> | + | |
− | </section>
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− |
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− |
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− | <section class="wrapper style4 container wow fadeInRight" style="margin-top:1em;">
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− | | + | |
− | <!-- Content --> | + | |
− | <div class="content">
| + | |
− | <section>
| + | |
− | <header>
| + | |
− | <h3>Working Safely... with Parts</h3>
| + | |
− | </header>
| + | |
− | <!-- TODO -->
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− |
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− | <p>All the parts we used throughout the project are NOT toxic or dangerous for humans or the environment. We neither worked with proteins toxic themselves, nor we used enzymes that synthesize toxic molecules. In addition to basic parts from the registry (i.e. RBS, promoters, β-carotene, etc), we also used two genes from the SAR86 uncultured bacteria. These genes are proteorhodopsin, which was extracted from the Registry (BBa_K773002) and blh (15,15’-β-carotene dioxygenase), which was synthesized by Genescript. There is no associated risk level with the uncultured bacteria SAR86, however we are using only DNA sequences that encodes for genes, which carry distinct functions that are no harmful to humans, or the environment. We have clarified this issue with iGEM Safety Office.</p>
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− | <p>We did <span class="i_enph">NOT</span> use any virulence factors in our organisms.</p>
| + | |
− | <p>The parts we used are to be considered safe as single parts and also as combined. Environmental dispersion of those parts would not represent a biological hazard or danger.</p>
| + | |
− |
| + | |
− | <header>
| + | |
− | <h3>Working Safely... with Organisms</h3>
| + | |
− | </header>
| + | |
− | <p>All the bacterial strains used are from Risk Group 1. We used the following strains:</p>
| + | |
− | <ul class="checklist">
| + | |
− | <li> One Shot® TOP10 Chemically Competent E. coli</li>
| + | |
− | <li> K12 - NEB 10-beta E. coli</li>
| + | |
− | <li> K12 - JM109 E. coli</li>
| + | |
− | <li> BL21 - NEB Express E. coli</li>
| + | |
− | </ul>
| + | |
− | </section>
| + | |
− | </div>
| + | |
− | </section> | + | |
− |
| + | |
− |
| + | |
− | <section class="wrapper style4 container wow fadeInLeft" style="margin-top:1em;">
| + | |
− | | + | |
− | <!-- Content -->
| + | |
− | <div class="content">
| + | |
− | <section>
| + | |
− | <header>
| + | |
− | <h3>Personal Protective Equipment (PPE)</h3>
| + | |
− | </header>
| + | |
− |
| + | |
− | <p>Last but non least we adopted the appropriate PPE during the lab work. We always used lab coats, nitrile gloves and goggles. We safely operated with biological material under the laminar flow cabinet BioAir SAFEMATE Series Class II 1.8.</p>
| + | |
− | <p>We used chemicals that are associated with hazard (ethidium bromide, azide, organic solvents) under the chemical hood Fume Hood MOD. ASEM® 120EN New - Class 0.</p>
| + | |
− | <p>We carefully revised all the Material Safety Datasheet of each compound that was used prior to start an experiment.</p>
| + | |
− | </section>
| + | |
− | </div>
| + | |
− | </section>
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− | | + | |
− | <!-- Two -->
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| </article> | | </article> |
− | <!-- END iGEM_Content_Section --> | + | |
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| <!-- Footer --> | | <!-- Footer --> |
| </html>{{UNITN-Trento/mainFooter}}<html> | | </html>{{UNITN-Trento/mainFooter}}<html> |
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− | </div> | + | </div> |
− | | + | <script> |
− | <!-- Scripts --> | + | function showAftPar(ob) |
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| + | jQuery(ob).addClass('spinnable'); |
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− | <script>new WOW().init();</script> | + | }else |
− | </div>
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| + | jQuery(ob).next().fadeOut(100,function(){jQuery(ob).removeClass('spinnable'); }); |
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| </div> | | </div> |
| + | </div> |
| </html> | | </html> |