Difference between revisions of "Team:UNITN-Trento/Test"

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<h2><strong>Interlab Study</strong></h2>
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<h2><strong>InterLab Measurement Study</strong></h2>
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<p>I have no idea what I'm doing!</p>
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<p>What happens when GFP meets different promoters? Are they all the same? Here is what we found out!</p>
 
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<li><p id="interlab_intro_btn" class="button small bstyle1" style="line-height:2em;" onclick="javascript:scrollToID('interlab_intro')"><span class="primary">Introduction</span> <span class="primary">&amp; Achievements</span></p></li> 
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<p>The characterization of standard parts has always been one of the main concerns in Synthetic Biology. For this very same reason, iGEM teams from all around the World were suggested to take part in the biggest Measurement Interlab Study ever conducted and the 2015 UNITN iGEM Team answered the call. The goal of this Second International Measurement Interlab Study is to assemble <b>three different devices</b>, each one containing a promoter with a screening plasmid intermediate and collect as many fluorescence data as possible. The three different promoters will differently affect the GFP production and thating plasmid intermediate and collect as iGEM teams are free to use any technique to measure their devices as long the obtained data are solid and reproducible.</p>
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<p>We used the three BioBrick devices listed in the &rdquo;Required Devices&rdquo; section.</p>
 
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<p>We used different techniques for measuring protein production, such as plate reader, spectrofluorimeter, FACS and for measuring transcript levels by using RT-qPCR</p>
 
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<p>We have three biological replicates for each measurement, with positive and negative controls</p>
 
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<p>We have technical replicates for each sample</p>
 
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<p>We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:</p>
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<p>We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.</p>
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Latest revision as of 14:24, 17 September 2015

InterLab Measurement Study

What happens when GFP meets different promoters? Are they all the same? Here is what we found out!

The Interlab Measurement Study