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| | <!-- Header --> | | <!-- Header --> |
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| + | <h2><strong>InterLab Measurement Study</strong></h2> |
| | + | <p>What happens when GFP meets different promoters? Are they all the same? Here is what we found out!</p> |
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| − | <p>Electron-production with <br /> engineered <i>E. coli</i> and <br /> <strong>proteorhodopsin</strong></p>
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| − | <h2>Electron production with <strong><i>E. coli</i></strong> and proteorhodopsin </h2>
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| − | <br />
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| − | <p>The aim of our project is to develop a pseudoautotrophic <span class="italicEnph">E. coli</span>, enabling it to live in the microbial fuel cell anaerobic condition. Our idea is to engineer E. coli with proteorhodopsin, a light-powered proton pump identified in bacteria that live in deep waters. This protein is activated by light and needs retinal molecules to pump protons outward following excitation. Thus we want to engineer our bacteria with blh, that encodes for the enzyme that cleaves beta-carotene and produces retinal.</p>
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| − | <p>To increase electron production we plan to overexpress pncB that represents the limiting step in the NAD+ synthesis pathway. The more NAD+ is produced, the more electrons are transported by NADH. In short: light activates proteorhodopsin, increases bacterial viability, and drives to a more efficient electron production.
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| − | Our bacterial device will give rise to a self-sustainable system, powered by sunlight during the day and by a light bulb at night. Daytime-electricity can be either used or stored to be employed as a power source for the lightbulb at night.</p>
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| − | <p class="pintest">Electricity from E. coli<br/>
| + | <section> |
| − | <span>Check out our amazing idea! Have fun!<br/> | + | <header> |
| − | <a class="btn" href="#">Project</a></span></p>
| + | <h3 class="wow fadeInDown">The Interlab Measurement Study</h3> |
| − | </li>
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| − | <li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/a/a0/Unitn_pics_slider_2.jpg'); ">
| + | |
| − | <p class="pintest">Electricity from E. coli<br/>
| + | |
| − | <span>Check out our amazing idea! Have fun!<br/>
| + | <div class="row"> |
| − | <a class="btn" href="#">Project</a></span></p>
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| + | </div> |
| − | <p class="pintest">Electricity from E. coli<br/>
| + | </div> |
| − | <span>Check out our amazing idea! Have fun!<br/>
| + | |
| − | <a class="btn" href="#">Project</a></span></p>
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| − | </li>
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| + | |
| − | <p class="pintest">Meet the team!<br/>
| + | |
| − | <span>We are UNITN iGEM 2015 and we are such essence as dNTPs are made on<br/>
| + | |
| | | | |
| − | <a class="btn" href="#"> To->Team </a></span></p>
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| − | </li>
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| − | <li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/0/04/Unitn_pics_slider_5.jpg'); ">
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| − | <p class="pintest">Catch 'em all!<br/>
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| − | <span>Many techniques, different strains and so much fluorescence… Enjoy the Measurement Interlab Study<br/>
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| | | | |
| − | <a class="btn" href="#"> To->Interlab </a></span></p>
| + | </section> |
| − | </li> | + | </div> |
| − | <li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/5/58/Unitn_pics_slider_6.jpg'); ">
| + | </section> |
| − | <p class="pintest">Synthetic Biology and mountains<br/>
| + | |
| − | <span>Admire our lovely city, surrounded by mountains. Trento is waiting for you<br/>
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| − | <a class="btn" href="#"> To->Interlab </a></span></p>
| + | |
| − | </li>
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| − | </ul>
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| − | <ol class="dots"><li class="dot active">1</li><li class="dot">2</li><li class="dot">3</li><li class="dot">4</li><li class="dot">4</li><li class="dot">4</li></ol></div>
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| − | <h2>Achievements</h2>
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