Difference between revisions of "Team:Nagahama/modeling"

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=Modeling Supported=
 
=Modeling Supported=
1.モデリング内容・設定
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==We were helped by [https://2015.igem.org/Team:Tokyo_Tech/Collaborations Tokyo-Tech]==
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[https://2015.igem.org/Team:Tokyo_Tech Tokyo-Tech] assisted us by constructing a model and running simulations.
  
・100×100×40[mm^3]の容器の底に、80×80×2[mm^3]のゲラニオールのシート(以下、ゲラニオールシート)を中央に置く。
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===Abstract===
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Our project is preserving food without cooling by flavor that ''E. coli'' produces
  
・24時間後に、最も濃度が薄いところで有効殺菌濃度0.04%※1を超える状況になるためのゲラニオールシートの濃度を求める。
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Therefore, We asked Team Tokyo-Tech to calculate the sufficient amount of culture fluid of ''E. coli'' which produce Geraniol (flavor) and time that Geraniol functions to preserve of the food in a certain lunch box.
  
・ゲラニオールシートには、大腸菌が48h生産したゲラニオールを濃縮して入れる。容器には米などの食材をしきつめているとする。大腸菌が48hで生産するゲラニオールは183mg/Lである。※2
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===Setting the situation and method===
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They constructed a model that They were preserving rice in a certain lunch box and laying the “Geraniol sheet” which contains concentrated Geraniol produced by ''E. coli'' on the bottom of the lunch box.
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Rice was packed in the lanch box and the Geraniol sheet put(8W X 8D X 2H) on the center of the bottom of lanch box.
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The Graniol sheet is 8W X 8D X 2H,It contains concentrated Geraniol produced by ''E. coli'' in a culture fluid.
  
・ゲラニオールの拡散係数は水で4.5×〖10〗^(-10) ["m" ^"2"  "/s]" ※3であるので、米はその1/5になると仮定し、モデリングを行った。
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They calculated the concentration of Geraniol in the sheet to surpass the effective bactericidal in the place where it was the lightest after 24 hours had passed since Graniol began to spread in lanch box.
  
・時間設定として理想は1時間であったが、拡散係数が小さく、容器の隅まで拡散するためには1時間は非常に少なかったため、24時間とした。
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===Result===
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[[File:Mmmlzuki.png|800px|thumb|center|Fig.  7-5-1-1.The State of Diffusion in the xy Plain at z=0[mm]]]
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[[File:Mmlzuki.png|800px|thumb|center|Fig. 7-5-1-2.The State of Diffusion in the yz Plain at x=50[mm]]]
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[[File:Mlzuki.png|800px|thumb|center|Fig. 7-5-1-3.The State of Diffusion in the xy Plain at z=40]]
  
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They used the culture fluid that E.coli were incubated for 48 hours. After the 48 hour of incubation, the culture fluid contained 183mg/L of Geraniol.
  
2.結果
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From their simulation, the culture fluid of E.coli should be concentrated 10,000 times in order to have the Geraniol concentration be above the effective bactericidal concentration even after laying the sheet for 24hours. The sufficient amount of culture fluid which they calculated in order to preserve rice in the box was 128L under our model.
 
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10000倍に濃縮したところ、最も薄い濃度のところで濃度が0.13%であった。
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この際、この容器一つ当たりに必要な培養液は、1〖"mm" 〗^"3" あたりに0.00183g(183g/Lをg/〖"mm" 〗^"3" に変換し、それを10000倍に濃縮)のゲラニオールが含まれているとすれば、128Lである。
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その際の拡散の様子は次のグラフのようになる。
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Figure 1は、底(z=0)での拡散の様子である。Figure 2は、x=50mmでのy方向、z方向の拡散の様子である。横軸がz軸であり、縦軸はy軸である。Figure 3は、上部(z=40)での拡散の様子である。グリッドの一目盛りは2mmである。
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 ただし、各図での色は対応していないので、同じ色でも値が異なっている。
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<html>
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<p>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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</p>
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<div class="highlightBox">
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<h4> Which other teams can we work with? </h4>
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<p>
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the <a hreef="https://2015.igem.org/Judging/Awards#Medals">iGEM team collaboration gold medal criterion</a>.
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</p>
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<p>
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In order to meet the gold medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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</p>
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</div>
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<p>
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Here are some suggestions for projects you could work on with other teams:
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</p>
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<ul>
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulating another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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</ul>
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</div>
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</html>
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Latest revision as of 14:57, 17 September 2015

Team Nagahama banner.jpg

Modeling Supported

We were helped by Tokyo-Tech

Tokyo-Tech assisted us by constructing a model and running simulations.

Abstract

Our project is preserving food without cooling by flavor that E. coli produces

Therefore, We asked Team Tokyo-Tech to calculate the sufficient amount of culture fluid of E. coli which produce Geraniol (flavor) and time that Geraniol functions to preserve of the food in a certain lunch box.

Setting the situation and method

They constructed a model that They were preserving rice in a certain lunch box and laying the “Geraniol sheet” which contains concentrated Geraniol produced by E. coli on the bottom of the lunch box. Rice was packed in the lanch box and the Geraniol sheet put(8W X 8D X 2H) on the center of the bottom of lanch box. The Graniol sheet is 8W X 8D X 2H,It contains concentrated Geraniol produced by E. coli in a culture fluid.

They calculated the concentration of Geraniol in the sheet to surpass the effective bactericidal in the place where it was the lightest after 24 hours had passed since Graniol began to spread in lanch box.

Result

Fig. 7-5-1-1.The State of Diffusion in the xy Plain at z=0[mm]
Fig. 7-5-1-2.The State of Diffusion in the yz Plain at x=50[mm]
Fig. 7-5-1-3.The State of Diffusion in the xy Plain at z=40

They used the culture fluid that E.coli were incubated for 48 hours. After the 48 hour of incubation, the culture fluid contained 183mg/L of Geraniol.

From their simulation, the culture fluid of E.coli should be concentrated 10,000 times in order to have the Geraniol concentration be above the effective bactericidal concentration even after laying the sheet for 24hours. The sufficient amount of culture fluid which they calculated in order to preserve rice in the box was 128L under our model.