Difference between revisions of "Team:Stockholm/Protocols"
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<a href="https://static.igem.org/mediawiki/2015/f/fb/STHLM_WB_protocol.pdf" >Western blot </a> | <a href="https://static.igem.org/mediawiki/2015/f/fb/STHLM_WB_protocol.pdf" >Western blot </a> | ||
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| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2015/7/7d/STHLM_Stripping_protocol.pdf" >Membrane stripping </a> |
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<a href="https://static.igem.org/mediawiki/2015/7/7b/STHLM_Spheroplast_protocol.pdf" >Spheroplast </a> | <a href="https://static.igem.org/mediawiki/2015/7/7b/STHLM_Spheroplast_protocol.pdf" >Spheroplast </a> | ||
Revision as of 17:08, 17 September 2015
Protocols
Western blotMembrane stripping
Spheroplast
FACS
Affinity separation (unfinished)
Dot blot
ELISA
Alternative osmolarity
Overlap PCR
Transformation
Colony PCR
Osmolarity
Site-directed mutagenesis
Adapted osmolarity
AHL osmolarity dependence
3A assembly
Chemically competent cells
Overnight cultures and restriction analysis
Traditional cloning (Restriction cloning)