Difference between revisions of "Team:Technion HS Israel/Project/Results"
Line 8: | Line 8: | ||
<a name="characterize of YFP"><h4>characterize of YFP</h4></a> | <a name="characterize of YFP"><h4>characterize of YFP</h4></a> | ||
<p>In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A). </p> | <p>In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A). </p> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/7/7f/Technion_HS_Israel_k1767010_2.jpg" | ||
+ | alt="Technion_HS_Israel_pre1" style="width:456px; | ||
+ | height:642px; border:solid; border-width:4px;" > | ||
</html> | </html> |
Revision as of 18:03, 17 September 2015
Results and disscussions
characterize of YFP
In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A).