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Revision as of 18:18, 17 September 2015

Technion 2015 HS Team's Wiki

Results and disscussions

  • characterize of YFP
  • characterize of YFP

    • characterize of YFP

    In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A).

    Technion_HS_Israel_pre1