Difference between revisions of "Team:Technion HS Israel/Project/Results"

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<p>In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A). </p>
 
<p>In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A). </p>
 
<img  src="https://static.igem.org/mediawiki/2015/1/17/Technion_HS_Israel_K1767010_3.png"
 
<img  src="https://static.igem.org/mediawiki/2015/1/17/Technion_HS_Israel_K1767010_3.png"
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<P>Scheme of BBa_1767010</P>
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<p>Scheme of BBa_1767010</p>
  
 
<P>This BioBrick (BBa_1767010) was used as a device that allows to</P>  
 
<P>This BioBrick (BBa_1767010) was used as a device that allows to</P>  

Revision as of 19:25, 17 September 2015

Technion 2015 HS Team's Wiki

Results and disscussions

  • characterize of YFP
  • characterize of YFP

    • characterize of YFP

    In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A).

    Technion_HS_Israel_pre1

    Scheme of BBa_1767010

    This BioBrick (BBa_1767010) was used as a device that allows to

    (1) determine the functionality of the promoter pLux

    (2) the formation of the active transcription factor LuxR in presence of AHL and

    (3) compare to a similar BioBrick that contains AiiA (BBa_K1767009).

    The BioBrick that does contain AiiA (LINK!!!), an AHL-inactivating enzyme, is expressed constitutively upstream of LuxR and degrades AHL hence the amount of AHL available for the formation of the active AHL-LuxR complex is limited. Decreasing active AHL-LuxR complexed leads to a reduced activation of the pLux promoter that should be observed in a reduced activation of YFP over time (Figure 1B).

    Technion_HS_Israel_pre1