Difference between revisions of "Team:Bielefeld-CeBiTec/Collaborations"
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| − | <h2>Collaboration with iGEM team | + | <h2>Collaboration with iGEM team <a href=" https://2015.igem.org/Team:Freiburg/Collaborations "_blank"> Freiburg 2015</a></h2> |
<p><img style="float: left; margin-right: 20px" src="https://static.igem.org/mediawiki/2015/5/58/Bielefeld-CeBiTec_Collaboration_iGEM_Freiburg_2015.jpg"width="150px" /> Our team from Bielefeld sent a plasmid based on BBa_I746909 that has a translation enhancing sequence (5’-UTR), and team Freiburg sent a plasmid containing turboYFP, a His and a Halo Tag. We would like to compare if these parts work in different cell free proteins synthesis environments.</p> | <p><img style="float: left; margin-right: 20px" src="https://static.igem.org/mediawiki/2015/5/58/Bielefeld-CeBiTec_Collaboration_iGEM_Freiburg_2015.jpg"width="150px" /> Our team from Bielefeld sent a plasmid based on BBa_I746909 that has a translation enhancing sequence (5’-UTR), and team Freiburg sent a plasmid containing turboYFP, a His and a Halo Tag. We would like to compare if these parts work in different cell free proteins synthesis environments.</p> | ||
<figcaption>Team Freiburg 2015</figcaption> | <figcaption>Team Freiburg 2015</figcaption> | ||
Revision as of 19:41, 17 September 2015
Collaborations
Because research is more fun together.
Collaboration with iGEM team Freiburg 2015
Our team from Bielefeld sent a plasmid based on BBa_I746909 that has a translation enhancing sequence (5’-UTR), and team Freiburg sent a plasmid containing turboYFP, a His and a Halo Tag. We would like to compare if these parts work in different cell free proteins synthesis environments.
Collaboration with team Dundee 2015
Some aspects of our project were similar to team Dundee. They built a chromium biosensor based on the same system we used, the chromate inducible operon of Ochrobactrum tritici 5bvl1. Therefore we decided to cooperate and exchanged ideas plasmid maps and sequences. During our collaboration, we realized we realized that they were comparing the original sequence of the repressor to one with the first 15 codons optimized. While our system bases on a complete codon optimized repressor. So we exchanged samples to test in each other’s systems. And look for differences. We were able to measure a combination of our promoter and their repressor in CFPS.
Collaboration with team Santa Clara 2015
As a part of our project, we provide a report about the dual use issue. An aspect of this report is the analysis of the legal situation in different countries, namely the applying laws in Germany, the European Union, and the United States of America. As an expert in the field of law, a member of team Santa Clara (Joseph Ayar, J.D. candidate from University of Santa Clara, School of Law) provided valuable insights in the applying laws and advisory boards in the USA. His analysis is marked as quote in our report.
Collaboration with team Exeter 2015
Similar to our project, team Exeter is working with a cell extract to translate proteins. They designed a synthetic toehold switch to detect specific RNA sequences. They aim to use that switch in a cell extract for further use in diagnostics. In terms of collaboration, we communicated via Skype and email. To help them to determine further applications of their project, we calculated the price for a single reaction of our crude cell extract in the size of their reaction and provided a table of contents.
We are thankful to team Exeter for reading our dual use report (available as PDF on our page) for language style.