Revision as of 21:43, 17 September 2015

iGEM Bielefeld 2015

Lab Notebook

Here you can find chronological notes of our lab work, sorted by subtopics.

Heavy metals

Here you find some detailed information about the lab work concerning the construction of our heavy metal biosensors in E.coli.You can see the difficulties in which we ran and follow our progress in creating our sensor systems.

Date rape drugs

The detailed process of the construction and characterization of the sensors detecting GBL, GHB and GABA in E. coli, is shown. You find our various methods to obtain the necessary elements from gene synthesis, A. tumifaciens and B. subtilis.

CFPS

In cell free protein synthesis (CFPS), the extracts of lysed cells, containing all the necessary components for transcription and translation, are used to produce proteins in vitro. Find out how we developed and optimized our own cell extract.

PRIA

See how we developed this alternative to standard biosensors, which is based on in vitro interaction of immobilized repressor proteins with plasmids containing the operator site corresponding to the repressor.

Miscellaneous

Here you can find chronological notes of our lab work besides the main topics, e.g. including the Measurement Interlab Study 2015.

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             <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/CFPS"><img class="img-rounded" src="https://static.igem.org/mediawiki/2015/9/9c/Bielefeld-Cebitec-Notebook-Minipic-CFPS_200x200.JPG" alt="CFPS" width="140" height="140">
            <h2>CFPS</h2>
-           <p>In cell free protein synthesis (CFPS), the extracts of lysed cells, containing all the necessary components for transcription and translation, are used to produce proteins <i>in vitro</i>.</p>
+           <p>In cell free protein synthesis (CFPS), the extracts of lysed cells, containing all the necessary components for transcription and translation, are used to produce proteins <i>in vitro</i>. Find out how we developed and optimized our own cell extract.</p>
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          </div>
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            <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/PlasmidDisplay"><img class="img-rounded" src="https://static.igem.org/mediawiki/2015/0/07/Bielefeld-Cebitec-Notebook-Minipic-PRIA_200x200.JPG" alt="Generic placeholder image" width="140" height="140">
            <h2>PRIA</h2>
-           <p>This alternative to standard biosensors is based on <i>in vitro</i> interaction of immobilized repressor proteins with plasmids containing the operator site corresponding to the repressor.</p>
+           <p>See how we developed this alternative to standard biosensors, which is based on <i>in vitro</i> interaction of immobilized repressor proteins with plasmids containing the operator site corresponding to the repressor.</p>
       </a>
          </div>

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