Difference between revisions of "Team:FAU Erlangen/Tour34"
| Line 11: | Line 11: | ||
Golden Gate | Golden Gate | ||
<ul> | <ul> | ||
| − | <li>we ordered a wrong enzyme with a way too high optimum | + | <li>we ordered a wrong enzyme with a way too high optimum --> reaction had very little production </li> |
<li>accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity </li> | <li>accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity </li> | ||
</ul> | </ul> | ||
Revision as of 23:09, 17 September 2015
Set backs: rpd3
- the rpd3 clones sent for sequencing always contained mutations
- we ordered a wrong enzyme with a way too high optimum --> reaction had very little production
- accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity
