Difference between revisions of "Team:FAU Erlangen/Tour34"
| Line 2: | Line 2: | ||
<html> | <html> | ||
| − | < | + | <h1>Set backs </h1> |
| − | rpd3 | + | <h3>rpd3 </h3> |
<ul> | <ul> | ||
<li>the rpd3 clones sent for sequencing always contained mutations </li> | <li>the rpd3 clones sent for sequencing always contained mutations </li> | ||
</ul> | </ul> | ||
| − | Golden Gate | + | <h3>Golden Gate </h3> |
<ul> | <ul> | ||
<li>we ordered a wrong enzyme with a way too high temperature optimum --> reaction had very little production </li> | <li>we ordered a wrong enzyme with a way too high temperature optimum --> reaction had very little production </li> | ||
Revision as of 23:11, 17 September 2015
Set backs
rpd3
- the rpd3 clones sent for sequencing always contained mutations
Golden Gate
- we ordered a wrong enzyme with a way too high temperature optimum --> reaction had very little production
- accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity
