Difference between revisions of "Team:Dundee/Part Collection"

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         <figcaption class="caption-center"><p> Characterisation of Haemoglobin B</p></figcaption>
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         <figcaption class="caption-center"><p> Characterisation of haemoglobin beta following SEC (size exclusion chromatography). A) The sample of concentrated fractions containing haemoglobin beta from nickel affinity purification was loaded onto a SEC column and the protein was eluted. B) 10µl of each fraction corresponding to the two observed peaks was mixed with 10µl of laemmli buffer and loaded onto a SDS gel (12.5% acrylamide). The bands observable on the gel are in line with the expected size of haemoglobin beta - 16kDa. C) Western blotting was then carried out against an anti-his antibody to confirm the presence of hHHB- His.</p></figcaption>
 
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      <figcaption class="caption-center"> Characterisation of haemoglobin beta following SEC (size exclusion chromatography). A) The sample of concentrated fractions containing haemoglobin beta from nickel affinity purification was loaded onto a SEC column and the protein was eluted. B) 10µl of each fraction corresponding to the two observed peaks was mixed with 10µl of laemmli buffer and loaded onto a SDS gel (12.5% acrylamide). The bands observable on the gel are in line with the expected size of haemoglobin beta - 16kDa. C) Western blotting was then carried out against an anti-his antibody to confirm the presence of hHHB- His. </figcaption>
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Revision as of 00:50, 18 September 2015

The Building Blocks of Our Project

Part Collection

Part:BBa_K1590000 (hHBA)

FluID- Blood Detection

Coding sequences for human Haemoglobin A. Forms a tetramer consisting of 2 A-chains and 2B-chains (See BBa_K1590001 for Haemboglobin B). Binds to Haptoglobin (BBa_K1590002). It is still being tested if Haptoglobin interacts with either single chain, or if it only binds to the complex.

Registry page for this part

Part:BBa_K1590001(hHBB)

FluID- Blood Detection

Coding sequences for human Haemoglobin B. Forms a tetramer consisting of 2 A-chains and 2B-chains (See BBa_K1590000 for Haemboglobin A). Binds to Haptoglobin (BBa_K1590002). It is still being tested if Haptoglobin interacts with either single chain, or if it only binds to the complex.

Registry page for this part

Characterisation of haemoglobin beta following SEC (size exclusion chromatography). A) The sample of concentrated fractions containing haemoglobin beta from nickel affinity purification was loaded onto a SEC column and the protein was eluted. B) 10µl of each fraction corresponding to the two observed peaks was mixed with 10µl of laemmli buffer and loaded onto a SDS gel (12.5% acrylamide). The bands observable on the gel are in line with the expected size of haemoglobin beta - 16kDa. C) Western blotting was then carried out against an anti-his antibody to confirm the presence of hHHB- His.

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Part:BBa_K1590002(hHBN)

FluID - Blood Detection

Haptoglobin is a binding protein with high affinity for free Haemoglobin.

Registry page for this part
Figure 1 - A random agarose gel showing some sort of banding

Lanosterol Synthase

Fingerprint Aging

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo.

Registry page for this part
Figure 1 - A random agarose gel showing some sort of banding

Lanosterol Synthase

Fingerprint Aging

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo.

Registry page for this part
Figure 1 - A random agarose gel showing some sort of banding

Lanosterol Synthase

Fingerprint Aging

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo.

Registry page for this part
Figure 1 - A random agarose gel showing some sort of banding

Lanosterol Synthase

Fingerprint Aging

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo.

Registry page for this part
Figure 1 - A random agarose gel showing some sort of banding

Lanosterol Synthase

Fingerprint Aging

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. Aenean ultricies mi vitae est. Mauris placerat eleifend leo.

Registry page for this part
Figure 1 - A random agarose gel showing some sort of banding