Difference between revisions of "Team:Tokyo Tech/Description"
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<h3 class="link"><a href="#ssrA">1. sstAのパート</a></h3> | <h3 class="link"><a href="#ssrA">1. sstAのパート</a></h3> | ||
<h3 class="link"><a href="#RNA">2. RNA thermometer</a></h3> | <h3 class="link"><a href="#RNA">2. RNA thermometer</a></h3> | ||
| + | <h3 class="link2"><a href="#21">2.1.“Background”for our characterization</a></h3> | ||
| + | <h3 class="link2"><a href="#22">2.2. Summary of the experiment</a></h3> | ||
| + | <h3 class="link2"><a href="#23">2.3. Result</a></h3> | ||
| + | |||
<h3 class="link"><a href="#Results">3. Results</a></h3> | <h3 class="link"><a href="#Results">3. Results</a></h3> | ||
<h3 class="link2"><a href="#Result1">3.1. Arabinose-dependent FimB (wild-type) expression</a></h3> | <h3 class="link2"><a href="#Result1">3.1. Arabinose-dependent FimB (wild-type) expression</a></h3> | ||
Revision as of 01:03, 18 September 2015
Description
contents
0. Summary
1. sstAのパート
2. RNA thermometer
2.1.“Background”for our characterization
2.2. Summary of the experiment
2.3. Result
3. Results
3.1. Arabinose-dependent FimB (wild-type) expression
3.2. FLA analysis
4. Discussion
5. Materials and Methods
5.1. Construction
5.2. Assay Protocol
5.2.1 Arabinose dependent FimB expression
5.2.2. FLA analysis
6. Reference
0. Summary
iGEM 2015 Tokyo_Tech improved the function of BBa_K395160 by submission of BBa_K1632020, and characterization of BBa_K1333309, to meet gold medal criteria.
Furthermore, we have improved characterization of another previously existing part, RNA thermometer (FourU) coding sequence plasmid (BBa_K1333309), constructed by iGEM 2014 SYSU-China by the following three points. (1) We measured the function of BBa_K1333309 at 42 ºC, which wasn’t confirmed by iGEM 2014 SYSU-China. (2) We clarified the processing of the background derived from Negative control. (3) We explicated that the measuring equipment was flow cytometer. (下2. RNA thermometerへのページ内リンク).