Difference between revisions of "Team:Gifu/team-profile"

Revision as of 01:22, 18 September 2015

Team Name:	Gifu (Visit their wiki) (Part Submissions)
Primary Contact (PI):	Hitoshi IWAHASHI
Schools:	Gifu University Yanagido, Gifu, Japan
Division:	iGEM	Application Date: 2015-04-27
Section:	Undergraduate
Region:	Asia	Acceptance Date: 2015-05-02 08:32:07
Description:

Team Status

Welcome to iGEM 2015 ! Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07 and your team registration fee has been received.

DNA Kit of Parts shipping information has been entered, thanks. View shipping information

Resource description has been submitted, thanks. View resource description

Your registration fee has been received. Thank you. More...

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Assigned Track: New Application

Title and AbstractMore...

Download a .pdf version

Circular mRNA ver2
In last year, we succeeded in designing the sequence which synthesizes circular mRNA and long chain protein in Escherichia coli. 　In this year, we had 2 purposes in our study. One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated complementary sequences around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved. The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated linker sequences into circular mRNA to synthesize the functional long-chain protein.

Team Roster

Instructors
aebihara2015	Akio Ebihara
isatoshi	Satoshi IWAMOTO
h1884	Hitoshi IWAHASHI

Student Members
ybanno	Yusuke Banno
nomuken	Nomura Kenta
momochi	Momoko YAMADA
kozakai	Kozakai Tomoya
Mori	Akihiro Moriyama
Yuki1201	Yuki Kamijo
SachiA	Sachi Asano
takema	Hasegawa Takema
fukufuku	Wataru Fukuda
Lagomutry	Ouchi Ryo
uyuu	Endo Yuki

This range of part numbers has been assigned to your team for use during the summer: BBa_K1332000 to BBa_K1332999

@@ Line 82: / Line 82: @@
 <table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Team Status</b><td align="center"><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_status" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
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-                 <b>Welcome to iGEM 2015 !</b> Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-04-23 12:49:42
+                 <b>Welcome to iGEM 2015 !</b> Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07
                  and your team registration fee has been received.</tr></tbody></table><div style="height: 3px;"></div><table_break></table_break><table width="800" id="table_status" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
 <tbody><tr><td><img src="https://igem.org/images/green_check.png"> DNA Kit of Parts shipping information has been entered, thanks.
@@ Line 102: / Line 102: @@
 <table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Title and Abstract</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2015.igem.org/Jamboree/Title_and_Abstract"><i>More...</i></a><td align="center"><a style="color: blue; text-decoration: none;" href="abstract_pdf_file.cgi?id=1332"><i>Download a .pdf version</i></a><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_abstract" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
-<tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA - the world's longest protein<br>
+<tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA ver2<br>
-<tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9"> Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-repeating proteins, massive　one, and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns and joining in exons after transcription. Splicing is catalyzed by several base sequences at both ends of intron as a ribozyme, being subjected to nucleophilic attack from introns to exons. We would like to clone the two parts of intron to use this mechanism and cyclize mRNA, making plasmids which include a gene coding proteins between those. And, by transforming <i>E.coli</i>, we get world’s longest proteins.
+<tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9"> &#12288;In last year, we succeeded in designing the sequence which synthesizes circular mRNA and long chain protein in
-</tr></tbody></table><br>
+Escherichia coli.<br>
+&#12288;In this year, we had 2 purposes in our study. One was an efficiency of the circularization. The efficiency was
+lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for
+splicing were far each other. So we incorporated complementary sequences around the ribozyme regions. We thought that
+the treatment brought two regions close and the efficiency of the circularization improved.<br>
+The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function
+because the folding of the proteins was broken. So we incorporated linker sequences into circular mRNA to synthesize the
+functional long-chain protein.<br></tr></tbody></table><br>
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