Difference between revisions of "Team:Gifu/team-profile"
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− | < | + | <tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA - the world's longest protein<br> |
− | < | + | <tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9"> Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-repeating proteins, massive one, and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns and joining in exons after transcription. Splicing is catalyzed by several base sequences at both ends of intron as a ribozyme, being subjected to nucleophilic attack from introns to exons. We would like to clone the two parts of intron to use this mechanism and cyclize mRNA, making plasmids which include a gene coding proteins between those. And, by transforming <i>E.coli</i>, we get world’s longest proteins. |
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Revision as of 03:31, 18 September 2015
TEAM PROFILE
Team Name: | Gifu | ||||||||
Primary Contact (PI): | Hitoshi IWAHASHI | ||||||||
Schools: | Gifu University Yanagido, Gifu, Japan | ||||||||
Division: | iGEM | Application Date: 2015-04-27 | |||||||
Section: | Undergraduate | ||||||||
Region: | Asia | Acceptance Date: 2015-05-02 08:32:07 | |||||||
Description: |
Team Status |
Welcome to iGEM 2015 ! Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07 and your team registration fee has been received. |
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Safety |
Check In FormAbout Our Lab FormAbout Our Project FormFinal Safety Form |
TrackMore... |
Assigned Track: New Application |
Title and AbstractMore... | Download a .pdf version |
Circular mRNA - the world's longest protein | ||||||||
Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-repeating proteins, massive one, and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns and joining in exons after transcription. Splicing is catalyzed by several base sequences at both ends of intron as a ribozyme, being subjected to nucleophilic attack from introns to exons. We would like to clone the two parts of intron to use this mechanism and cyclize mRNA, making plasmids which include a gene coding proteins between those. And, by transforming E.coli, we get world’s longest proteins. |
Team Roster |
Instructors | |||
h1884 | Hitoshi IWAHASHI | ||
isatoshi | Satoshi IWAMOTO | ||
aebihara2014 | Akio Ebihara |
Student Members | |||
momochi | Momoko YAMADA | ||
Kairi | Kairi ISHIMARU | ||
nomuken | Nomura Kenta | ||
kozakai | Kozakai Tomoya | ||
fumiki | Fumiki Izumi | ||
liyu | son liyu | ||
FreshLemon | Haruka Maruyama | ||
takema | Hasegawa Takema | ||
ybanno | Yusuke Banno | ||
Mori | Akihiro Moriyama | ||
fukufuku | Wataru Fukuda | ||
SachiA | Sachi Asano |
This range of part numbers has been assigned to your team for use during the summer: BBa_K1859000 to BBa_K1859999 |