Difference between revisions of "Team:Gifu/team-profile"

Latest revision as of 03:36, 18 September 2015

Team Name:	Gifu (Visit their wiki) (Part Submissions)
Primary Contact (PI):	Hitoshi IWAHASHI
Schools:	Gifu University Yanagido, Gifu, Japan
Division:	iGEM	Application Date: 2015-04-27
Section:	Undergraduate
Region:	Asia	Acceptance Date: 2015-05-02 08:32:07
Description:

Team Status

Welcome to iGEM 2015 ! Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07 and your team registration fee has been received.

DNA Kit of Parts shipping information has been entered, thanks. View shipping information

Resource description has been submitted, thanks. View resource description

Your registration fee has been received. Thank you. More...

Safety

Check In Form About Our Lab Form About Our Project Form Final Safety Form

TrackMore...

Assigned Track: New Application

Title and AbstractMore...

Download a .pdf version

Circular mRNA ver.2.0
In last year, we succeeded in designing the sequence which synthesizes circular mRNA and long chain protein in Escherichia coli. 　In this year, we had 2 purposes in our study. One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated complementary sequences around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved. The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated linker sequences into circular mRNA to synthesize the functional long-chain protein.

Team Roster

Instructors
h1884	Hitoshi IWAHASHI
isatoshi	Satoshi IWAMOTO
aebihara2014	Akio Ebihara

Student Members
momochi	Momoko YAMADA
Kairi	Kairi ISHIMARU
nomuken	Nomura Kenta
kozakai	Kozakai Tomoya
fumiki	Fumiki Izumi
liyu	son liyu
FreshLemon	Haruka Maruyama
takema	Hasegawa Takema
ybanno	Yusuke Banno
Mori	Akihiro Moriyama
fukufuku	Wataru Fukuda
SachiA	Sachi Asano

This range of part numbers has been assigned to your team for use during the summer: BBa_K1859000 to BBa_K1859999

@@ Line 73: / Line 73: @@
 <table width="800" id="table_info" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
 <tbody><tr><td width="150">Team Name: <td style="line-height: 150%; font-size: 150%; font-weight: 800;" colspan="9">Gifu<div style="padding-left: 40px; font-size: 12px; font-weight: normal; display: inline;">
-             <a href="https://2014.igem.org/Team:Gifu">(Visit their wiki)</a><a style="padding-left: 20px;" href="http://partsregistry.org/cgi/dna_transfer/batch_list.cgi?group_id=1707"> (Part Submissions)</a></div><tr><td>Primary Contact (PI): <td colspan="9">Akio Ebihara<tr><td style="vertical-align: top;">Schools:<td colspan="9"><pre style="background: none; padding: 0px; border: currentColor; border-image: none; width: 90%;">Gifu University
+             <a href="https://2015.igem.org/Team:Gifu">(Visit their wiki)</a><a style="padding-left: 20px;" href="http://partsregistry.org/cgi/dna_transfer/batch_list.cgi?group_id=1707"> (Part Submissions)</a></div><tr><td>Primary Contact (PI): <td colspan="9">Hitoshi IWAHASHI<tr><td style="vertical-align: top;">Schools:<td colspan="9"><pre style="background: none; padding: 0px; border: currentColor; border-image: none; width: 90%;">Gifu University
-Yanagido, Gifu, Japan</pre><tr><td>Division:<td style="width: 300px;">iGEM<td>Application Date:&nbsp; 2014-02-17<tr><td>Section:<td style="width: 300px;">Undergraduate<tr><td>Region:<td style="width: 300px;">Asia<td>Acceptance Date:  2014-04-23 12:49:42<tr><td>Description:<td colspan="9">
+Yanagido, Gifu, Japan</pre><tr><td>Division:<td style="width: 300px;">iGEM<td>Application Date:&nbsp; 2015-04-27<tr><td>Section:<td style="width: 300px;">Undergraduate<tr><td>Region:<td style="width: 300px;">Asia<td>Acceptance Date:  2015-05-02 08:32:07<tr><td>Description:<td colspan="9">
 </tr></tbody></table><br>
@@ Line 80: / Line 80: @@
-<table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Team Status</b><td align="center"><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_status" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
+<TABLE width='800' border='0' cellspacing='0' cellpadding='0' style='margin-left:auto; margin-right:auto; border: none;'><TR><TD nowrap width='30%' style='padding-right: 2em;text-align: left;'><B>Team Status</B><TD align='center'><TD width='30%' align='right'></TABLE><TABLE id='table_status' width='800' border='0' cellspacing='0' cellpadding='0' style='margin-left:auto; margin-right:auto; border: 1px solid gray;background-color: #dcdcdc; padding-left: 10px;padding:10px 15px 0px 15px;line-height: 150%; padding-right:2px;padding-top:3px;' >
-<tbody><tr><td style="color: rgb(0, 102, 0);">
+<TR><TD style='color:#006600;font-size:120%,padding:5px 0 5px 0'>
-                 <b>Welcome to iGEM 2014 !</b> Your iGEM 2014 team application was accepted by iGEM Headquarters on 2014-04-23 12:49:42
+                 <B>Welcome to iGEM 2015 !</B> Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07
-                 and your team registration fee has been received.</tr></tbody></table><div style="height: 3px;"></div><table_break></table_break><table width="800" id="table_status" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
+                 and your team registration fee has been received.</TABLE><DIV style='height: 3px'></DIV><TABLE id='table_status' width='800' border='0' cellspacing='0' cellpadding='0' style='margin-left:auto; margin-right:auto; border: 1px solid gray;background-color: #dcdcdc; padding-left: 10px;padding:10px 15px 0px 15px;line-height: 150%; padding-right:2px;padding-top:3px;' >
-<tbody><tr><td><img src="https://igem.org/images/green_check.png"> DNA Kit of Parts shipping information has been entered, thanks.
+<TABLE_BREAK><TR><TD><img src='https://igem.org/images/green_check.png'> DNA Kit of Parts shipping information has been entered, thanks.
-                  <a href="Shipping.cgi?group_id=1707"><i> View shipping information</i></a><tr><td><img src="https://igem.org/images/green_check.png"> Resource description has been submitted, thanks.
+                  <A href='Shipping.cgi?group_id=2287'><I> View shipping information</I></A><TR><TD><img src='https://igem.org/images/green_check.png'> Resource description has been submitted, thanks.
-                  <a href="Resource_Description.cgi?group_id=1707"><i> View resource description</i></a><tr><td><img src="https://igem.org/images/green_check.png"> Your registration fee has been received. Thank you.
+                  <A href='Resource_Description.cgi?group_id=2287'><I> View resource description</I></A><TR><TD><img src='https://igem.org/images/green_check.png'> Your registration fee has been received. Thank you.
-                  <a href="https://2014.igem.org/Team_Registration_Fee"><i> More...</i></a><table_break>
+                  <A href='https://2015.igem.org/Team_Registration_Fee'><I> More...</I></A><TABLE_BREAK>
-</table_break></tr></tbody></table><br>
+</TABLE><BR>
+<!-- safety -->
+<TABLE width='800' border='0' cellspacing='0' cellpadding='0' style='margin-left:auto; margin-right:auto; border: none;'><TR><TD nowrap width='30%' style='padding-right: 2em;text-align: left;'><B>Safety</B><TD align='center'><TD width='30%' align='right'></TABLE><TABLE id='table_safety' width='800' border='0' cellspacing='0' cellpadding='0' style='margin-left:auto; margin-right:auto; border: 1px solid gray;background-color: #dcdcdc; padding-left: 10px;padding:10px 15px 0px 15px;line-height: 150%; padding-right:2px;padding-top:3px;' >
+<TR><TD colspan=9><A style='padding-left:40px' href='https://2015.igem.org/Safety/Check_In?team_id=1859'>Check In Form</A><A style='padding-left:40px' href='https://2015.igem.org/Safety/About_Our_Lab?team_id=1859'>About Our Lab Form</A><A style='padding-left:40px' href='https://2015.igem.org/Safety/About_Our_Project?team_id=1859'>About Our Project Form</A><A style='padding-left:40px' href='https://2015.igem.org/Safety/Final_Safety_Form?team_id=1859'>Final Safety Form</A>
+</TABLE><BR>
 <!-- tracks -->
-<table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Track</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2014.igem.org/Jamboree/Track_Selection"><i>More...</i></a><td align="center"><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_tracks" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
+<table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Track</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2015.igem.org/Jamboree/Track_Selection"><i>More...</i></a><td align="center"><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_tracks" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
 <tbody><tr><td colspan="9">Assigned Track: New Application
 </tr></tbody></table><br>
@@ Line 100: / Line 111: @@
-<table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Title and Abstract</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2014.igem.org/Jamboree/Title_and_Abstract"><i>More...</i></a><td align="center"><a style="color: blue; text-decoration: none;" href="abstract_pdf_file.cgi?id=1332"><i>Download a .pdf version</i></a><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_abstract" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
+<table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Title and Abstract</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2015.igem.org/Jamboree/Title_and_Abstract"><i>More...</i></a><td align="center"><A style='text-decoration: none; color: blue;'
+             HREF='abstract_pdf_file.cgi?id=1859' ><i>Download a .pdf version</i></a><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_abstract" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
-<tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA - the world's longest protein<br>
+<tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA ver.2.0<br>
-<tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9"> Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-repeating proteins, massive　one, and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns and joining in exons after transcription. Splicing is catalyzed by several base sequences at both ends of intron as a ribozyme, being subjected to nucleophilic attack from introns to exons. We would like to clone the two parts of intron to use this mechanism and cyclize mRNA, making plasmids which include a gene coding proteins between those. And, by transforming <i>E.coli</i>, we get world’s longest proteins.
+<tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9"> &#12288;In last year, we succeeded in designing the sequence which synthesizes circular mRNA and long chain protein in <i>Escherichia coli</i>.<br>
-</tr></tbody></table><br>
+&#12288;In this year, we had 2 purposes in our study. One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated <b>complementary sequences</b> around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved.<br>
+The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated <b>linker sequences</b> into circular mRNA to synthesize the functional long-chain protein.<br></tr></tbody></table><br>
 <style>
 .form_roster td { font-size:95% }
@@ Line 165: / Line 181: @@
 <tbody><tr><td style="padding: 3px 0px;"><b>Instructors</b>
+<!-- Non-editable Member 24452 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21012">h1884</a><td style="width: 200px;">Hitoshi IWAHASHI<td><td>
-<!-- Non-editable Member 23221 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=19993">aebihara2014</a><td style="width: 200px;">Akio Ebihara<td><td>
 <!-- Non-editable Member 23933 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20610">isatoshi</a><td style="width: 200px;">Satoshi IWAMOTO<td><td>
-<!-- Non-editable Member 24452 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21012">h1884</a><td style="width: 200px;">Hitoshi IWAHASHI<td><td></tr></tbody></table><div style="height: 3px;"></div><table_break>
+<!-- Non-editable Member 23221 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=19993">aebihara2014</a><td style="width: 200px;">Akio Ebihara<td><td>
+</tr></tbody></table><div style="height: 3px;"></div><table_break>
 </table_break><table width="800" id="table_roster" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
 <tbody><tr><td style="padding: 3px 0px;"><b>Student Members</b>
-<!-- Non-editable Member 23894 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20590">ybanno</a><td style="width: 200px;">Yusuke Banno<td><td>
+<!-- Non-editable Member 23896 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20592">momochi</a><td style="width: 200px;">Momoko YAMADA<td><td>
-<!-- Non-editable Member 23895 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20591">nomuken</a><td style="width: 200px;">Nomura Kenta<td><td>
+<!-- Non-editable Member 23899 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=26709">Kairi</a><td style="width: 200px;">Kairi ISHIMARU<td><td>
-<!-- Non-editable Member 23896 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20592">momochi</a><td style="width: 200px;">Momoko YAMADA<td><td>
+<!-- Non-editable Member 23895 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20591">nomuken</a><td style="width: 200px;">Nomura Kenta<td><td>
 <!-- Non-editable Member 23897 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20593">kozakai</a><td style="width: 200px;">Kozakai Tomoya<td><td>
-<!-- Non-editable Member 23898 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20594">Mori</a><td style="width: 200px;">Akihiro Moriyama<td><td>
+<!-- Non-editable Member 24477 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=26712">fumiki</a><td style="width: 200px;">Fumiki Izumi<td><td>
-<!-- Non-editable Member 23899 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20596">Yuki1201</a><td style="width: 200px;">Yuki Kamijo<td><td>
+<!-- Non-editable Member 24761 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=26710">liyu</a><td style="width: 200px;">son liyu<td><td>
-<!-- Non-editable Member 23900 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20595">SachiA</a><td style="width: 200px;">Sachi Asano<td><td>
+<!-- Non-editable Member 24761 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=26711">FreshLemon</a><td style="width: 200px;">Haruka Maruyama<td><td>
 <!-- Non-editable Member 23901 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20597">takema</a><td style="width: 200px;">Hasegawa Takema<td><td>
+<!-- Non-editable Member 23894 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20590">ybanno</a><td style="width: 200px;">Yusuke Banno<td><td>
+<!-- Non-editable Member 23898 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20594">Mori</a><td style="width: 200px;">Akihiro Moriyama<td><td>
 <!-- Non-editable Member 24451 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21011">fukufuku</a><td style="width: 200px;">Wataru Fukuda<td><td>
-<!-- Non-editable Member 24477 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21022">Lagomutry</a><td style="width: 200px;">Ouchi Ryo<td><td>
+<!-- Non-editable Member 23900 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20595">SachiA</a><td style="width: 200px;">Sachi Asano<td><td>
-<!-- Non-editable Member 24761 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21240">uyuu</a><td style="width: 200px;">Endo Yuki<td><td>
 </tr></tbody></table><br>
 <!-- ranges -->
@@ Line 201: / Line 228: @@
 <table width="800" id="table_ranges" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
-<tbody><tr><td>This range of part numbers has been assigned to your team for use during the summer: <span style="margin-left: 10px;">BBa_K1332000 to BBa_K1332999</span>
+<tbody><tr><td>This range of part numbers has been assigned to your team for use during the summer: <span style="margin-left: 10px;">BBa_K1859000 to BBa_K1859999 </span>
 </tr></tbody></table><br>
 <!-- end content -->
@@ Line 225: / Line 252: @@
 <ol id="index">
 <li><a href=" https://2015.igem.org/Team:Gifu/Team/#member" style="text-decoration:none;" >TEAM MEMBERS &nbsp; </a></li>
-<li><a href="#instructor" style="text-decoration:none;" >INSTRUCTOR &nbsp; </a></li>
+<li><a href=" https://2015.igem.org/Team:Gifu/Team/#instructor" style="text-decoration:none;" >INSTRUCTOR &nbsp; </a></li>
-<li><a href="#att"style="text-decoration:none;" >ATTRIBUTION &nbsp; </a></li>
+<li><a href=" https://2015.igem.org/Team:Gifu/Team/#att"style="text-decoration:none;" >ATTRIBUTION &nbsp; </a></li>
-<li><a href="https://2015.igem.org/Team:Gifu/team-profile"style="text-decoration:none;" >Team Profile &nbsp; </a></li>
+<li><a href="https://2015.igem.org/Team:Gifu/team-profile"style="text-decoration:none;" >TEAM PROFILE &nbsp; </a></li>
   <br>
 </ol>