Difference between revisions of "Team:SPSingapore/Notebook-Week-13"

Line 246: Line 246:
  
 
<! ------------------------------- Footer START ------------------------------------------------>
 
<! ------------------------------- Footer START ------------------------------------------------>
 +
  
 
<div id="footer1">
 
<div id="footer1">
Line 251: Line 252:
 
<div class="left_sec">
 
<div class="left_sec">
 
<p>
 
<p>
<img src = "https://static.igem.org/mediawiki/2015/a/ab/SPSingapore_Team-Logo-New.png" height = "80px">
+
<img src = "https://static.igem.org/mediawiki/2015/a/ab/SPSingapore_Team-Logo-New.png" height = "60px" style="margin-top:5px;margin-left:-130px;">
 
</p>
 
</p>
 
</div>
 
</div>
 
<div class="right_sec">
 
<div class="right_sec">
 
<p>
 
<p>
<img src = "https://static.igem.org/mediawiki/2015/0/05/SPSingapore_NUS-Logo.png" height = "60px" style = "margin-top:10px;margin-right:30px;padding-right:30px;border-right: 3px solid lightgrey;">
+
<img src="https://static.igem.org/mediawiki/2015/0/05/SPSingapore_NUS-Logo.png" height="50px" style="margin-top:10px;margin-right:30px;padding-right:30px;border-right: 3px solid lightgrey;">
<img src = "https://static.igem.org/mediawiki/2015/7/72/SPSingapore_SPS-Logo.png" height = "60px" style = "margin-top:10px;margin-right:50px;"></a>
+
<img src="https://static.igem.org/mediawiki/2015/7/72/SPSingapore_SPS-Logo.png" height="50px" style="margin-top:10px;margin-right:30px;">
 
</p>
 
</p>
 
</div>
 
</div>

Revision as of 03:53, 18 September 2015


Research Notebook

Week 13 (16/8 - 22/8)

▪ Aug 18

Anaerobic Promoter    ☀ Yi Han ☀

PCR for the clones of pNirB-gfp from B21(1-4) and dH5alpha (1-4)with pNirB-gfp-F/R primers
5ul plasmid, 5ul H2O in each tube

PCR Mastermix
dNTP 8ul
MgCl2 20ul
Taq Polymerase 2ul
Buffer 40ul
F/R primers 0.8ul each
H2O 40ul


▪ Aug 19

Anaerobic Promoter    ☀ Chi Yan ☀

Colony PCR for pEFYP plated on (17/8)
PCR Master mix For pEYFP
H2O 13.25ul
Buffer 5ul
dNTP 5ul
F/R primer 5ul
2.5mM MgCl2 12.5
GoTaq 11.25ul


PCR for pNirBgfp as with (18/8) with pNirB-gfp primers, Biobricks prefix and suffix primers
(Melting temperature increased to 53 degrees)
3ul of plasmid in each tube

PCR Master mix for pNirB-gfp primers and BB prefix/suffix primers
H2O 101.25ul
Buffer 45ul
dNTP 9ul
Forward Primer 9ul
Reverse Primer 9ul
2.5mM MgCl2 22.5ul
GoTaq polymerase 2.25ul