Difference between revisions of "Team:CU Boulder"

 
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{{CU_Boulder}}
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[[File:Example.jpg]]{{CU_Boulder}}
 
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<article>
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<body>
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    <!-- front image -->
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<div style="text-align: center">
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<img src="https://static.igem.org/mediawiki/2015/6/6b/CU-Boulder-temp-cu-photo.gif" text-align="center" alt="CU-Boulder"/>
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<div>
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<!----------- front image ------------->
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<div id="stripe_1">
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<div class="margins">
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    <div id="CU_Naph">
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        <h2>UNIVERSITY OF COLORADO</h2>
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        <h2>NAPHTHALENE RECORDERS</h2>
 
     </div>
 
     </div>
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    <div class="image"></div>
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</div>
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<div id="stripe_2">
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<div class="margins">
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    <br><br>
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    <br>
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    <h3>A Big Fracking Deal</h3>
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    <!--<h4>A Big Fracking Deal</h4>-->
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</div>
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</div>
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<div id="quick_nav">
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<div id="quick_nav_container">
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    <div class="quick_nav_box" id=nav_box1">
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        <h5><a href="https://2015.igem.org/Team:CU_Boulder/project/motivation">Problem</a></h5>
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    </div>
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    <div class="quick_nav_box" id=nav_box2">
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        <h5><a href="https://2015.igem.org/Team:CU_Boulder/Description">Solution</a></h5>
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    </div>
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    <div class="quick_nav_box" id=nav_box3">
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        <h5><a href="https://2015.igem.org/Team:CU_Boulder/project/results">Results</a></h5>
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    </div>
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</div>
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</div>
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<p></p>
 
<p></p>
 
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         <!--<p class="box-float-right">I am a sample box. I hold content and look, I have a shadow. I am also personified for some reason. I am not as awesome as the Fact Sphere.</p>-->
 
         <!--<p class="box-float-right">I am a sample box. I hold content and look, I have a shadow. I am also personified for some reason. I am not as awesome as the Fact Sphere.</p>-->
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      <h1>Abstract</h1>
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        <p>Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds.  In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water.  To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking.  Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase.  Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.</p>
 
  
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<h1>Abstract</h1>
  
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<p>Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds.  In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water.  To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking.  Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase.  Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.</p>
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{{CU_Boulder_footer}}
 
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Latest revision as of 04:48, 18 September 2015

File:Example.jpg


<!DOCTYPE html> Team:CU_Boulder - 2015.igem.org

UNIVERSITY OF COLORADO

NAPHTHALENE RECORDERS




A Big Fracking Deal

Abstract

Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds. In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water. To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking. Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase. Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.

Team:CU-Boulder - 2015.igem.org