Difference between revisions of "Team:XJTLU-CHINA/Team"

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                         <ul>
 
                         <ul>
 
                             <li><a class="jump1" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J1">Map description</a></li>
 
                             <li><a class="jump1" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J1">Map description</a></li>
−
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/DescriptionJ2">RNAT</a></li>
+
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J2">RNAT</a></li>
 
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J4">Improve experiment of RNAT</a></li>
 
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J4">Improve experiment of RNAT</a></li>
 
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J4">Chromo-protein testing</a></li>
 
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J4">Chromo-protein testing</a></li>
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                     </li>
 
                     </li>
 
                     <li class="mainNavi col1">
 
                     <li class="mainNavi col1">
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                         <div id="PrimaryNaviBg3" style="background-color: #3399CC";>
 
                             <a class="primaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts">Parts</a>
 
                             <a class="primaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts">Parts</a>
 
                         </div>
 
                         </div>
 
                         <ul>
 
                         <ul>
−
                             <li><a class="jump1" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts#J1">Modularity</a></li>
+
                             <li><a class="jump1" href="#J1">Modularity</a></li>
−
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts#J2">Part Detail</a></li>
+
                             <li><a class="jump2" href="#J2">Part Detail</a></li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li class="mainNavi col1">
 
                         <div id="PrimaryNaviBg4">
 
                         <div id="PrimaryNaviBg4">
 
                             <a class="primaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Team">Team</a>
 
                             <a class="primaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Team">Team</a>
 
                         </div>
 
                         </div>
 
                         <ul>
 
                         <ul>
−
                             <li><a class="jump1" href="#J1">Team introduction</a></li>
+
                             <li><a class="jump1" href="https://2015.igem.org/Team:XJTLU-CHINA/Team#J1">Team introduction</a></li>
−
                             <li><a class="jump2" href="#J2">Attribution</a></li>
+
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Team#J2">Attribution</a></li>
 
<li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Team#J3">Achievements</a></li>
 
<li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Team#J3">Achievements</a></li>
 
                         </ul>
 
                         </ul>
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                         <ul>
 
                         <ul>
 
                             <li><a class="jump1" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J1">Map description</a></li>
 
                             <li><a class="jump1" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J1">Map description</a></li>
−
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/DescriptionJ2">RNAT</a></li>
+
                             <li><a class="jump2" href="#J2">RNAT</a></li>
−
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J4">Improve experiment of RNAT</a></li>
+
                             <li><a class="jump2" href="#J4">Improve experiment of RNAT</a></li>
−
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Description#J4">Chromo-protein testing</a></li>
+
                             <li><a class="jump2" href="#J4">Chromo-protein testing</a></li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
 
                     <li class="ZmainNavi col1">
 
                     <li class="ZmainNavi col1">
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                         <div id="ZPrimaryNaviBg3">
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                         <div id="ZPrimaryNaviBg3" style="background-color: #3399CC";>
 
                             <a class="ZprimaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts">Parts</a>
 
                             <a class="ZprimaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts">Parts</a>
 
                         </div>
 
                         </div>
 
                         <ul>
 
                         <ul>
−
                             <li><a class="jump1" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts#J1">Modularity</a></li>
+
                             <li><a class="jump1" href="#J1">Modularity</a></li>
−
                             <li><a class="jump2" href="https://2015.igem.org/Team:XJTLU-CHINA/Parts#J2">Part Detail</a></li>
+
                             <li><a class="jump2" href="#J2">Part Detail</a></li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
 
                     <li class="ZmainNavi col1">
 
                     <li class="ZmainNavi col1">
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                         <div id="ZPrimaryNaviBg4">
 
                             <a class="ZprimaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Team">Team</a>
 
                             <a class="ZprimaryNavi" href="https://2015.igem.org/Team:XJTLU-CHINA/Team">Team</a>
 
                         </div>
 
                         </div>
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     </div>
 
     </div>
 
</div>
 
</div>
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<div class="firstPage">
 
<div class="firstPage">
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    <div class="videoBg">
 
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        <br/>
 
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    </div>
 
 
     <div class="firstPageBg">
 
     <div class="firstPageBg">
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        <video autoplay poster="https://static.igem.org/mediawiki/2015/thumb/b/b2/MovPostXJTLU15.png/800px-MovPostXJTLU15.png" loop id="bgvid">
 
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            <source src="https://static.igem.org/mediawiki/2015/3/34/MP4.mp4" type="video/mp4">
 
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        </video>
 
 
     </div>
 
     </div>
 
     <div class="container">
 
     <div class="container">
 
         <div class="overview">
 
         <div class="overview">
 
             <div class="title">
 
             <div class="title">
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                 <p class="heading">Team</p>
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                 <p class="heading">Part</p>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
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         <div class="container">
 
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             <div class="lv1">
 
             <div class="lv1">
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                 <h2>Team introduction</h2>
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                 <h2>Modularity</h2>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
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                 <div class="lv3">
 
                 <div class="lv3">
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                     <h4>Instructor & Sponsor</h4>
+
                     <p>After the team had finished the genetic design of Land, Coast, Marine, and Polar bacteria, time was almost running out and there were still some concerns on the performance promoters and ribothermometers. To make sure we could have a chance to test the uncertain parts without delaying the whole circuits’ synthesis, instructor suggested us to modularize genetic circuits and then manipulate the modules using Gibson Assembly.</p>
 
                     <br/>
 
                     <br/>
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                     <p>The team divided modules into two groups. One is “promoter + operator/ RBS/Ribothermometer”. The other one is “Coding sequence + terminator”. Details are in the following form. </p>
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                     <img class="teamImg" src="https://static.igem.org/mediawiki/2015/3/3f/TeamImg1XJTLU15.jpg">
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                    <div class="teamImgText">
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                        <p>Guoxia Han</p>
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−
                        <p>Hello, world champions! You are the creators of new world, while I am just one of the supporters for you guys. No matter how many rewards or how much honor I achieved, I might be your student in iGEM competition. I know the team experienced a difficult time because of permit and sponsor. Hence, I deal with these issues and encourage you to focus on the project. Here I appreciate all team members in XJTLU-CHINA iGEM team. It is them that create a wonderful project. They merit it!</p>
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                    <div class="teamImgText">
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                        <p>Dechang Xu</p>
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−
                        <P>Hello, I am Da Xu. I have supported iGEM teams for 5 years. This year I participate in XJTLU-CHINA iGEM team. I give some useful advice on mathematical modeling and experiment plan. I am very happy to help these young adults do what they want. Also, I hope they could achieve a good result.</P>
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                        <br/>
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                    <img class="teamImg" src="https://static.igem.org/mediawiki/2015/0/06/Teamimg3.jpeg">
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                    <div class="teamImgText">
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                        <p>Yunpeng Zhong</p>
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−
                        <P>Hi, I am Yunpeng. I join in the XJTLU-CHINA team as lab instructor and sponsor. I manage a synbio company in Suzhou Industry Park. I am very interested in iGEM competition and would like to support their experiment with funding and technology. After a hot summer, I am very proud to these smart and hard-working teenagers. </P>
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                        <p>Yacheng Guan</p>
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                        <P>Hello, I am Yacheng and you can also call me ‘Er Ye’. I work in a synbio company and mainly devote to protein engineering. Very glad to meet these students and we have learned lots of new knowledge together. I hope XJTLU-CHINA team could gain wonderful results! </P>
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                     <p>Actually in Land, Coast, Marine, and Polar bacteria there are several overlaps in the composition of genetic circuits. For instance, marine and polar bacteria both contain blue chromoprotein gene; coast and land bacteria both contain yellow chromoprotein gene. Each repeating part is only shown as one module.</p>
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                        <p>Yuwei Han</p>
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                        <P>Hi, I am yuwei of XJTLU biological science year three. I am 21, and I am sure this is the most exciting and challenging competition I’ve ever participated in. Before iGEM, I also had internships in other institutes. Thus, I am responsible for the experiment parts. For me, the iGEM competition is a big opportunity for me to make a breakthrough of myself. It is not only about a great deal of new knowledge, technique and experience I can get from the experiment. Moreover, it gives me a chance to actually participate in the generation of an innovation, from the development of a new idea to finally give rise to the final product. </P>
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                        <p>Jiongjian Li</p>
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                        <P>Hello, I’ m Jiongjian Li, a second year student from the XJTLU’s Biology major. This is my first year to join iGEM and it is great to join this competition. The best thing in this world is thinking about new idea. That is what I do in this team. Also, I am willing to work with other teammates to achieve our goals. </P>
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                        <p>Tiange Li</p>
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                        <P>Hi I’m Tiange Li (Mag) and this is my first tour of iGEM. I bet say it is one of the most extraordinary things happened in my year 2 biology study. When there is a chance to make an effort on a self-designed experiment, everything taught in lectures becomes vivid and the discoveries made before just sound more exciting. I like the idea of genetic circuit and fortunately this is what we are heading for. Up to now what I’ve done most are literature research and paper work, I fresh myself when going through research articles. Thanks for my teammates' help; I now do it more efficiently. I really enjoy the time spending with them, sharing information and taking a walk from the lab. So...just hope we can finally make it!</P>
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                        <p>Yakun Tang </p>
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                        <P>Hello everyone! I’m Yakun Tang and I’m a year 2 student in Biological department of XJTLU. This is the first time I participate in iGEM, so for me, this project is like a gate which leads to a magic biology world. During the participation of this project, I learn much more knowledge than that from books, and many skills such as literature reviewing skills and experimental skills are quickly improved. More importantly, I meet and know our teammates who are all very capable and full of enthusiasm and I learn a lot from them. My work in our iGEM team is dealing with finanicial affairs as well as conducting some experiments. Both of these two need patience and carefulness, but as a smart girl^0^, I will try my best!</P>
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                        <p>Chenghao shen</p>
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                        <P>Hello, everyone! I’m Harrison Shen studying in XJTLU, majored in biological sciences, for 3 years and there is one year left for my graduation. How time flies! It is a great pleasure to work as a team during our project. Considered taking the advantage of my computer skills, I am responsible for team poster, brief video production and wiki page beautification. The iGEM is a worldwide competition and joining this not only broadens my horizons and improves my knowledge but also meets new friends and strengthens our friendship. Engaging in synthetic biology, all we need is to come up with more imaginative ideas to create human-friendly designs for our lives. Just do it! I believe future world will be dominated by biotechnology.</P>
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                        <p>Ziang Shi</p>
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                        <P>Hello everyone, my name is Ziang Shi. And I’m studying computer science in Xi’an Jiaotong-Liverpool University. It is my pleasure for non-biology student to join iGEM team. I really hope to use my programming knowledge to contribute our team in constructing wiki as well as giving advises in dealing with data. As a young undergraduate, iGEM provide a valuable opportunity to practice our skills and learn extra knowledge in a short period time. In addition, it is a memorable experience to work with friend for the same goal. I will keep working our enjoyable project to help team get best result.</P>
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                        <p>Wenbo Xu</p>
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                        <P>Hi, I am James. I am biology science student in XJTLU. I am proud to participate in iGEM competition this summer. Maybe I am a staggering genius and always do something different. But, at this time I will cooperate with my teammates and solve difficult problems in any aspect of our project. I believe our iGEM team will be different because of me as well as every teammate.</P>
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                        <p>Hao Wu </p>
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                        <P>Hey, I am Hao Wu and I am a student majoring in the biology science in Xi'an Jiaotong-Liverpool University. I worked in the RNA thermometer group and focus on the design of riboswitch. I have learned a lot and gained valuable experience. Also I had a deeper understand of synthetic biology. Among the six months, although we have come across plenty of difficulties, we still enjoy this unforgettable experience. I'm glad to be one of the iGEMers and looking forward to meeting others all over the world.</P>
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                        <p>Xinhao Wang</p>
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                        <P>Hi everyone! I’m Xinhao Wang and that’s my first year in XJTLU iGEM Team. I’m a year 2 student studying Biology at Xian JiaoTong-Liverpool University and I really enjoy the work during our project. As a member of our team I work with my teammates to develop the innovative ideas and devote myself to the lab work. Through iGEM my knowledge of synthetic biology was greatly enriched and I learnt valuable skills in this field. During the designing and conducting of of our project, our attitude of doing science is becoming more careful and thorough.  IGEM is a great experience for us science students to combine our ideas and practical lab work. We are all working and giving our best for our project.</P>
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                        <p>Yizhou Wang</p>
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                        <P>Hi everyone! I’m Yizhou Wang, a XJTLUer major in biology science, and that’s my first time participating in iGEM competition. In a amazing team, I really enjoy the time spent with other team members during our project. As a team member, conducting experiments is my major task in which caution and preciseness keep in my mind all the time. iGEM is a gate for me to formally enter in research field. I don’t want to struggle for gold medal but rather build relationships and learn incredible knowledge as well as recognizing how “things” are created from parts by measures of synthetic biology. We strive for perfection with our wisdom and capture the everyone’s light of wisdom to perfect perfection.</P>
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                        <p>Wang Zixu</p>
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                        <P>My name is Wang Zixu, member of XJTLU igem team. You can also call me William. It is a great honor to take part in this competition where I learned a lot about synthetic biology and made lots of new friends.</P>
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                        <p>Yinsiyi Wang</p>
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                        <P>Hello, everyone! I am Yinsiyi.Wang, a photographer in XJTLU-CHIAN iGEM team. Although I am an urban planning graduate and I know iGEM is synbio competition, however I still can help our team in human practice part. I take picture and video to record our work in summer. I am very happy to join them and also I want to make new friends in Boston. Giant jamboree I am coming! </P>
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                        <p>Tianhua Zhai</p>
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                        <P>Hello everyone! I am Tianhua.Zhai. I am a biology student in Xi’an Jiaotong-Liverpool University, which is an international and novel university in China. This year I join the iGEM-XJTLU team to fight for the gold medal in iGEM competition. I am responsible for writing our wiki and supporting lab group in our team. Also, I work for human practice to let public know iGEM and synbio. iGEM is a fantastic and challengeable competition, I feel really excited and pleased to participate in it. I would like to learn how to work in a team and make true friends. I believe that there is no royal road to science, and only those who do not dread the fatiguing climb of its steep paths have a chance of gaining its luminous summits.</P>
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                        <p>Zhe Yang</p>
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                        <P>I’m Zhe Yang and second year biological student of Xi’an Jiaotong Liverpool University. This is my first time to know iGEM competition and lead a team to participant. I really enjoy the work during the project. There is a story that I know this competition from my internship supervisor in March and I begin to study synthetic biology. During this project, I learn knowledge in synthetic biology and it gives me experience of designing and conducting an experiment. I really enjoy this summer and have many friends. I think iGEM is a good opportunity for students to study synthetic biology together and communicate with students from different universities. It is lucky to be here, iGEM.</P>
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                     <h4>Synbio Tech</h4>
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                     <p>Synbio Tech is an innovator in the field of synthesis biology in China, having a wide range of cooperation with both international companies and universities. It aims to provide customers with a perfect technical platform of the synthesis biology. For now, by applying their unique self-established technical platform Syno® involving sound chip-based gene synthesis and high throughput gene sequencing service, it already develops the technology of constructing recombinant antibody libraries as well as highly-purified vaccines, genome synthesis and DNA storage.</p>
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                     <P>Firstly, the modules would be directly inserted to the plasmid pSB1C3, so that the team could hand in new parts immediately after characterizing them. </P>
 
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                     <p>As a major sponsor of XJTLU-China 2015, they provided a lab equipped with all apparatus and reagents needed and directly gave a hand in the de novo gene synthesis in the circuits’ construction. At the beginning of iGEM project, the company offered us a training program including both theoretical basis of synthesis biology and practical skills useful in later wet experiments. Also during the collaborate this summer, they were active in supervising our experiments and giving valuable suggestions on experiment protocols and result analysis.</p>
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                     <P>Secondly, this method ensures us to get the perfect circuits as soon as possible. It makes constructing long designed genetic circuit a more flexible process compared to the original gene synthesis and it is just the same way as an engineer welds all electronic components together to make a circuit board with expected function. Moreover, in this case, we didn’t need to repeat constructing the same modules used in different circuits. </P>
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                     <P>Thirdly, Gibson assembly is capable for successful assembly of multiple DNA fragments. Hence, verified modules can later be integrated with others quickly. </P>
 
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                    <p>As an instructor of XJTLU-CHINA iGEm team, Dr. Han did nothing on our project as we can instruct themselves. He believed we were able to perform well in their lab work and outside activities. Before this competition, he had told us how to work in a team and do scientific research. Particularly, we had learned critical thinking and diligence from him. Hence, this time, only thing he did for us was communicating with some authorities in university to obtain their support. He trusts we can face difficulties and solve problems.</p>
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                    <P>He helped us for constructing chromoprotein test’s plasmids and answered the questions about designing the plasmid and experiment.</P>
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                    <p>Yunpeng Zhong</p>
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                    <P>Yunpeng put forward the idea of modularize the parts in circuits for convenient test of uncertain parts. He also helped us evaluate the feasibility of our map design and gave us experiment room to do our project.</P>
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                    <P>Dechang Xu joined us in early July 2015 as a project advisor. He have given valuable suggestions on mathematical modeling and kept reminding the team for the important criteria that IGEM awarding requires.</P>
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                    <p>Chenghao was in charge of major designs of promotional materials, including making posters, banners, videos and PPT etc., anything about propagating our project both in giant jamboree and human practice. In addition, he also modified photos and pictures to beautify our wiki.</p>
 
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                    <br/>
 
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                    <p>Tiange Li</p>
 
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                    <p>Tian’ge did plenty literature research on genetic regulatory parts and have gave valuable suggestions regarding the problems we meet in wet experiment.  She also participated in designing and testing part of Ribothermometers and recorded this part of experiment on our notebook in detail during July, 2015.</p>
 
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                    <br/>
 
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                    <p>Wenbo Xu</p>
 
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                    <p>Wenbo was in authority of circuits design. He collected ideas and suggestions from teammates and constructed the DNA frame of our biothermomap. Mathematical modeling of this project was conducted by him with Xinhao’s support. He also did the work of sponsors contacting, safety form submitting and parts registration.</p>
 
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                    <br/>
 
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                    <p>Yakun Tang</p>
 
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                    <p>Yakun participated in and conducted the lab work during June, July and August. She also managed financial affairs and was responsible for items purchasing such as team uniforms. She wrote chromoprotein testing part that we put on our wiki and did human practice, such as participating iGEM meet up in Shanghai Science and Technology </p>
 
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                    <br/>
 
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                    <p>Yizhou Wang</p>
 
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                    <p>Yizhou participated in the first phase of our experiments (from June to August) and helped in reviewing literature on regulator parts that used in our project. He also contributed in writing the wiki part of ribothermometers, tet operator and so on. He did part of the work of human practices also i.g. The meetup in the Shanghai Science and Technology Museum</p>
 
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                    <br/>
 
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                    <p>Zhe Yang</p>
 
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                    <p>Zhe was responsible for monitoring the work of team in multiple aspects including experiment operating, human practices conducting, wiki designing, and so on. She participated in the lab work throughout this summer (From June to September) and was involved in designing the experiments for determining the degradation rate of chromoproteins and the validity of RNA thermometers (From June to September). She also contributed in notebook recording and result analysis.</p>
 
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                    <br/>
 
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                    <p>Yinsiyi Wang</p>
 
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                    <p>She is photographer and associated art designer in XJTLU-CHIAN. She took pictures and video in every event in human practice. She helped wiki editor to decorate our website and supported video editor to make microfilms for showing iGEM.</p>
 
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                    <br/>
 
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                    <p>Tianhua Zhai</p>
 
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                    <p>In April, he joined in a team held by several year 2 students who were curious about iGEM competition and then help to establish their iGEM team, the XJTLU-CHINA. In May, in an iGEM public speech he talked about a project about synthesis of crispr-cas system in gliomas cell to specifically knock out CD44 gene for inhibiting cell migration and activation. The team achieved permit and funding from university after the speech. In June, he always participated in discussion and wrote a iGEM procedure in order to achieve further funding from a synbio company. In summer, he supported lab work. He had communication with other iGEM teams in shanghai workshop and organized many human practice events. </p>
 
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                    <br/>
 
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                    <p>Jiongjian Li</p>
 
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                    <p>Jiongjian designed the second version of our RNA thermometers and tested them, improved the function of our first version of RNA thermometers. He also participated in the molecular cloning experiments in August and September.  </p>
 
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                    <br/>
 
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                    <p>Xinhao Wang</p>
 
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                    <p>Xinhao conducted the first and third round of RNA thermometer experiments. She also participated in molecular cloning experiments in July and September.</p>
 
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                    <br/>
 
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                    <p>Gandi Yao</p>
 
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                    <p>Gandi responsible for produceing the pictures put on wiki. Such as icons and sketch maps. He is also responsible for the uniform designing, the pictures of the poster.</p>
 
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                    <br/>
 
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                    <p>Ziang Shi</p>
 
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                    <p>Ziang is responsible for constructing wiki webpages as programmer. He also worked as wiki webpages art design with the help of Gandi.</p>
 
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                    <br/>
 
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                    <p>Zixu Wang</p>
 
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                    <p>Zixu designed the original pathway of our project together with Gandi. He also designed and operated the experiment of the "constitutive promoter + tet operator "system. Moreover, he took part in the meet up at Shanghai Science and Technology Museum. Meanwhile, he did some lab work and wrote something that we put on our wiki.</p>
 
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                    <br/>
 
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                    <p>Yuwei Han</p>
 
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                    <p>She helped doing the lab work, mainly in the experiments of picking and testing the function ribothermometer during July and August. She also participated in the molecular cloning experiment during that time.</p>
 
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Revision as of 07:24, 18 September 2015

Part

XJTLU-CHINA

XJTLU-CHINA

Part


Modularity

After the team had finished the genetic design of Land, Coast, Marine, and Polar bacteria, time was almost running out and there were still some concerns on the performance promoters and ribothermometers. To make sure we could have a chance to test the uncertain parts without delaying the whole circuits’ synthesis, instructor suggested us to modularize genetic circuits and then manipulate the modules using Gibson Assembly.


The team divided modules into two groups. One is “promoter + operator/ RBS/Ribothermometer”. The other one is “Coding sequence + terminator”. Details are in the following form.


Actually in Land, Coast, Marine, and Polar bacteria there are several overlaps in the composition of genetic circuits. For instance, marine and polar bacteria both contain blue chromoprotein gene; coast and land bacteria both contain yellow chromoprotein gene. Each repeating part is only shown as one module.

How can modularity help?


Firstly, the modules would be directly inserted to the plasmid pSB1C3, so that the team could hand in new parts immediately after characterizing them.


Secondly, this method ensures us to get the perfect circuits as soon as possible. It makes constructing long designed genetic circuit a more flexible process compared to the original gene synthesis and it is just the same way as an engineer welds all electronic components together to make a circuit board with expected function. Moreover, in this case, we didn’t need to repeat constructing the same modules used in different circuits.


Thirdly, Gibson assembly is capable for successful assembly of multiple DNA fragments. Hence, verified modules can later be integrated with others quickly.