Difference between revisions of "Team:Bielefeld-CeBiTec/Organisms"
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<h1 style="margin-bottom: 0px">Organisms</h1> | <h1 style="margin-bottom: 0px">Organisms</h1> | ||
| − | <p> | + | <p>The favorite pets of a synthetic biologist</p> |
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="Subtitle"> | + | <div class="Subtitle" id="Ecoli"> |
<h2><i>Escherichia coli</i></h2> | <h2><i>Escherichia coli</i></h2> | ||
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| − | <img src="https://static.igem.org/mediawiki/2015/d/d6/Bielefeld-CeBiTec_Ecoli.jpg"></a> | + | <img src="https://static.igem.org/mediawiki/2015/d/d6/Bielefeld-CeBiTec_Ecoli.jpg" width="300px"></a> |
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| − | <p> We used following | + | <p> We used the following <i>E. coli</i> strains during our project:</p> |
| − | + | <ul> | |
| − | + | <li> KRX (Source: Promega)</li> | |
| − | + | <ul> | |
| − | <ul | + | |
<li> Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.</li> | <li> Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.</li> | ||
| + | |||
</ul> | </ul> | ||
| − | + | <li> ER2566 (T7 Express Competent E. coli (High Efficiency), source: New England Biolabs) </li> | |
| − | <li> ER2566 (T7 Express Competent | + | <ul> |
| − | <ul | + | |
<li> Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] </li> | <li> Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] </li> | ||
| − | + | </ul> | |
| − | </ul> | + | </ul> |
| − | </ | + | <p> Some other strains were used just once to investigate their performance in <i>in vitro</i> cell-free protein synthesis:</p> |
| − | + | ||
| − | + | <ul> | |
| − | + | <li> Rosetta-gami 2 (Source: Novagen, part of Merck KGaA) </li> | |
| + | <ul> | ||
| + | |||
| + | <li> Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)</li> | ||
| + | </ul> | ||
| + | <li> BL21 (DE3) (Source: New England Biolabs)</li> | ||
| + | <ul> | ||
| + | <li> Genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
<div class="Subtitle"> | <div class="Subtitle"> | ||
<h2><i>Bacillus subtilis</i></h2> | <h2><i>Bacillus subtilis</i></h2> | ||
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| − | <p> In our project we used <i>B. subtilis</i> strain 168 with genotype <i>trpC2</i>. You can find further information about the origin of this strain in <a href="#Zeigler2008">Zeigler et al.</a></p> | + | <p> In our project we used <i>B. subtilis</i> strain 168 with genotype <i>trpC2</i> for the GABA operon sequence. You can find further information about the origin of this strain in <a href="#Zeigler2008">Zeigler et al.</a></p> |
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| − | <img src="https://static.igem.org/mediawiki/2015/e/e5/Bielefeld-CeBiTec_Agrobacterium-tumefaciens.png" width=" | + | <img src="https://static.igem.org/mediawiki/2015/e/e5/Bielefeld-CeBiTec_Agrobacterium-tumefaciens.png" width="300px"></a> |
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| − | <p> We isolated | + | <p> We isolated the blc-operon from <i>A. tumefaciens</i> C58.1. For detailed information about this organism please be referred to <a href="#Wood2001">Wood et al. 2001</a>. </p> |
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Latest revision as of 11:03, 18 September 2015
Organisms
The favorite pets of a synthetic biologist
Escherichia coli
We used the following E. coli strains during our project:
- KRX (Source: Promega)
- Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.
- ER2566 (T7 Express Competent E. coli (High Efficiency), source: New England Biolabs)
- Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm]
Some other strains were used just once to investigate their performance in in vitro cell-free protein synthesis:
- Rosetta-gami 2 (Source: Novagen, part of Merck KGaA)
- Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)
- BL21 (DE3) (Source: New England Biolabs)
- Genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
Bacillus subtilis
In our project we used B. subtilis strain 168 with genotype trpC2 for the GABA operon sequence. You can find further information about the origin of this strain in Zeigler et al.
Agrobacterium tumefaciens
We isolated the blc-operon from A. tumefaciens C58.1. For detailed information about this organism please be referred to Wood et al. 2001.
References
Wood, D. W.; Setubal, J. C.; Kaul, R.; Monks, D. E.; Kitajima, J. P.; Okura, V. K. et al. (2001): The genome of the natural genetic engineer Agrobacterium tumefaciens C58. In: Science (New York, N.Y.) 294 (5550), S. 2317–2323. DOI: 10.1126/science.1066804.
Zeigler, Daniel R.; Prágai, Zoltán; Rodriguez, Sabrina; Chevreux, Bastien; Muffler, Andrea; Albert, Thomas et al. (2008): The origins of 168, W23, and other Bacillus subtilis legacy strains. In: Journal of bacteriology 190 (21), S. 6983–6995. DOI: 10.1128/JB.00722-08.
