Difference between revisions of "Team:Technion Israel/Lab Notebook/Protocols"

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<h1><b>Protocols</b></h1>
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<p>Colony PCR</br>
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<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols/Cloning">Cloning</h1></a>
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<ol><li><a href="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_protocols_colonyPCR.pdf" target="_blank"><i>E. coli</i></a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf" target="_blank"><i>B. subtilis</i></a></li></ol></p>
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<a href="https://static.igem.org/mediawiki/2015/c/c8/Technion_Israel_2015_protocols_ligation.pdf" target="_blank">Ligation</a></br></br>
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<a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><i>Bacillus</i> Secretion Verification</a>
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</br></br><a href="https://static.igem.org/mediawiki/2015/7/7e/Technion_Israel_2015_protocols_3aHSDactivity.pdf" target="_blank">3ɑ-HSD Activity Verification Protocol</a>
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</br></br><a href="https://static.igem.org/mediawiki/2015/2/22/Technion_Israel_2015_protocol_NADPHfluorescence.pdf" target="_blank">Extracellular NADPH Estimation</a></br></br>
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<a href="https://static.igem.org/mediawiki/2015/4/47/Technion_Israel_2015_newLysisMethodComparison.pdf" target="_blank">Comparison of Lysis Procedures for NADPH Measurement</a>
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</br></br><a href="https://static.igem.org/mediawiki/2015/1/14/Technion_Israel_2015_protocols_shelf-life.pdf" target="_blank">Shelf-life and Storage Conditions Estimation</a>
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Latest revision as of 12:24, 18 September 2015

Team: Technion 2015

Home
Home
Project
Lab Notebook
Human Practices
Judging Criteria
Team
UP
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Protocols

Gel Preparation

Sigma-Aldrich Miniprep

Primer Dilution

CIP

Gibson Assembly

Restriction

UV Gel Viewing

Gel Extraction

Transformation

  1. E.coli
  2. B.subtilis

Colony PCR

  1. E. coli
  2. B. subtilis

Ligation

Nanodrop Use

Bacillus Secretion Verification

3ɑ-HSD Activity Verification Protocol

Extracellular NADPH Estimation

Comparison of Lysis Procedures for NADPH Measurement

Shelf-life and Storage Conditions Estimation

Contact Us