Difference between revisions of "Team:Technion Israel/Lab Notebook/Protocols"

 
(37 intermediate revisions by 3 users not shown)
Line 5: Line 5:
 
 
 
<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Technion/CSS?action=raw&ctype=text/css" />
 
<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Technion/CSS?action=raw&ctype=text/css" />
−
+
<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Technion/navbar/css?action=raw&ctype=text/css" />
 
<style type="text/css">
 
<style type="text/css">
−
.body {
+
body {
−
background-image: url("https://static.igem.org/mediawiki/2015/7/73/Team_Technion_2015_Background_Orange.jpg");
+
background-color: rgb(202, 174, 244);
 
}
 
}
−
+
header{background-image: url("https://static.igem.org/mediawiki/2015/2/28/Technion_Israel_2015_protocols.jpg"); background-size: 100%; background-repeat: no-repeat; width: 100%; height: 400px; margin-bottom: 0px;}
−
#protocol_instructions
+
.title h1{ color: rgb(202, 174, 244); font-weight: bold; font-size: 100px; text-align: center; background: rgba(0, 0, 0, 0.8); font-family: sans-serif; font-style: Arial; height: 100px; text-shadow: black 6px 6px 6px; line-height: 80px;}
−
{width: 900px;
+
#bodyContent h1{margin-bottom: 0em;}
−
margin: auto;
+
.mini-title{background-image: url("https://static.igem.org/mediawiki/2015/9/96/Technion_Israel_2015_dna.jpg"); background-size: 100%;  background-repeat: no-repeat; width: 100%; height: 200px; margin-bottom: 0px;}
−
padding: 10px;
+
.content{padding: 20px; margin-top: 0px; font-size: 16px;}
−
background-color: white;
+
.mini-title2{background-image: url("https://static.igem.org/mediawiki/2015/b/b6/Technion_Israel_2015_proofof.jpg"); background-size: 100%; background-repeat: no-repeat; width: 100%; height: 200px; margin-bottom: 0px;}
−
font-size: 16}
+
−
+
 
−
#DNA_protocols
+
.doubleColumns{
−
{position: absolute;
+
  display:table;
−
width: 150px;
+
  border-spacing:12px;
−
top:35%;
+
  min-height:200px;
−
left:25%
+
  width: 100%;
−
}
+
  margin: auto;
−
+
}
−
#DNA_protocols h1
+
 
−
{font-size: 24;
+
.doubleColumn{
−
text-align: center;
+
  display:table-cell;
−
float: top;}
+
  width:48%; padding-left: 150px;
−
+
}
−
#Medium_protocols
+
 
−
{position: absolute;
+
.DNA{display: block; width: 100%;}
−
width: 150px;
+
.up_button img { width: 130px;}
−
top: 800px;
+
 
−
left: 75%;
+
</style>
−
}
+
<title>Team: Technion 2015</title>  
−
#Medium_protocols h1{font-size: 24;
+
−
text-align: center;}
+
−
+
−
#Comb_protocols h1{font-size: 24;
+
−
text-align: center;}
+
−
#Comb_protocols
+
−
{position: absolute;
+
−
width: 100px;
+
−
top: 800px;
+
−
left: 60%;
+
−
}
+
−
+
−
#Proof_protocols
+
−
{position: absolute;
+
−
width: 100px;
+
−
top: 800px;
+
−
left: 20%;
+
−
}
+
−
#Proof_protocols h1{font-size: 24;
+
−
text-align: center;
+
−
float: left;}
+
−
+
−
#Misc_protocols
+
−
{position: absolute;
+
−
top: 500px;
+
−
left: 35%;
+
−
}
+
−
+
−
</style>
+
−
+
−
<title>Team: Technion 2015</title>
+
 
</head>
 
</head>
 
<body>
 
<body>
  
−
<header>
+
<!-------------------------------------nav-bar---------------------------------------------->
 +
<div class="nav_menu">
  
−
<div class="main_menu">
+
<a href="https://2015.igem.org/Team:Technion_Israel" style="position:fixed; width: 170px; display:inline-block; text-decoration: none;" class="nav_home_link">
−
<nav>
+
<img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id="nav_home" /><div class="home_text">Home</div></a>
−
<a href="#">
+
 
−
<button class="button_main1">
+
<div class="main_buttons">
−
<span class="btn-text">Project</span>
+
<div id="project" class="main_button">Project</div>
−
<span class="btn-bg"></span>
+
<div id="Lab_Notebook" class="main_button">Lab&nbsp;Notebook</div>
−
</button>
+
<div id="Human_Practices" class="main_button">Human&nbsp;Practices</div>
−
</a>
+
<div id="Judging_Criteria" class="main_button">Judging&nbsp;Criteria</div>
−
<a href="#">
+
<div id="Team" class="main_button">Team</div>
−
<button class="button_main2">
+
−
<span class="btn-text">Lab Notebook</span>
+
−
<span class="btn-bg"></span>
+
−
</button>
+
−
</a>
+
−
<a href="#">
+
−
<button class="button_main3">
+
−
<span class="btn-text">Judging Criteria</span>
+
−
<span class="btn-bg"></span>
+
−
</button>
+
−
</a>
+
−
<a href="#">
+
−
<button class="button_main4">
+
−
<span class="btn-text">Human Practices</span>
+
−
<span class="btn-bg"></span>
+
−
</button>
+
−
</a>
+
−
<a href="#">
+
−
<button class="button_main5">
+
−
<span class="btn-text">Team</span>
+
−
<span class="btn-bg"></span>
+
−
</button>
+
−
</a>
+
−
</nav>
+
 
</div>
 
</div>
 +
<nav>
 +
<div id="Team_Links" class="main_links">
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us" id="Team_Links_About_Us">About&nbsp;Us</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Attributions" id="Team_Links_Attributions">Attributions</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Team/Sponsors" id="Team_Links_Sponsors">Sponsors</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Team/gallery" id="Team_Links_gallery">Gallery</a>
 +
</div>
 +
<div id="Judging_Criteria_Links" class="main_links">
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Parts" id="Judging_Criteria_Links_Parts">Parts</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria/Achievements" id="Judging_Criteria_Links_Achievements">Achievements</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Collaborations" id="Judging_Criteria_Links_Collaborations">Collaborations</a>
 +
</div>
 +
<div id="Human_Practices_Links" class="main_links">
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Practices">Practices</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Practices/safety">Safety</a>
 +
</div>
 +
<div id="Lab_Notebook_Links" class="main_links">
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Overview">Overview</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols">Protocols</a>
 +
</div>
 +
<div id="project_Links" class="main_links">
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Overview">Overview</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Secretion">Secretion</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Expression">Expression</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Cofactor">Cofactor</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Design">Comb</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Modeling">Modeling</a>
 +
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Results">Results</a>
 +
</div>
 +
</nav>
 +
</div>
 +
<!-------------------------------------nav-bar-end------------------------------------------>
 +
 +
<!-------------------------------------up-button-------------------------------------------->
 +
<div class="up_button" style="text-decoration: none;">
 +
<a href="#" class="up_link" style="text-decoration: none;">
 +
    <div class="up_button_text" style="text-decoration: none;">UP</div>
 +
    <img src="https://static.igem.org/mediawiki/2015/7/7a/Technion_2015_Up_hippie.png" alt="Scroll to top" />
 +
</a>
 +
</div>
 +
<!-------------------------------------up-button-end---------------------------------------->
 +
<header>
 
</header>
 
</header>
 +
<div class="title">
 +
<h1><b>Protocols</b></h1>
 +
</div>
  
−
<!---------------------------------------dropdown menu----------------------------------->
+
<div class= "content">
−
<div class="dropdown">
+
<div class="DNA">
−
<a href="https://2015.igem.org/Team:Technion_Israel">
+
<div class="mini-title">
−
<img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id="home_nav" />
+
</div>
−
<div id="home_text">Navigate</div>
+
<hr></hr>
−
</a>
+
<div class="doubleColumns">
−
<nav>
+
  <div class="doubleColumn">
−
<div class="main1">
+
    <a href="https://static.igem.org/mediawiki/2015/b/b8/Techion_Israel_2015_protocols_gelpreparation.pdf" target="_blank">Gel Preparation</a></br></br>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Project">Project</a>
+
<a href="https://static.igem.org/mediawiki/2015/b/ba/Technion_Israel_2015_protocols_miniprep.pdf" target="_blank">Sigma-Aldrich Miniprep</a></br></br>
−
<span class="btn-bg1"></span>
+
<a href="https://static.igem.org/mediawiki/2015/b/bb/Technion_iGEM_2015_Primer_dilution_protocol.pdf" target="_blank">Primer Dilution</a></br></br>
−
<div class="sub1">
+
<a href="https://static.igem.org/mediawiki/2015/c/cb/Technion_Israel_2015_protocols_CIP.pdf" target="_blank">CIP</a></br></br>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Overview">Overview</a>
+
<a href="https://static.igem.org/mediawiki/2015/2/26/Technion_Israel_2015_protocols_gibson.pdf" target="_blank">Gibson Assembly</a></br></br>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Bacillus">Bacillus</a>
+
<a href="https://static.igem.org/mediawiki/2015/2/23/Technion_Israel_2015_protocols_restriction.pdf" target="_blank">Restriction</a></br></br>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Project/E_Coli">E.Coli</a>
+
<a href="https://static.igem.org/mediawiki/2015/1/1d/Technion_Israel_2015_protocols_UVviewing.pdf" target="_blank">UV Gel Viewing</a></br></br>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Project/NADPH">NADPH</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Comb">Comb</a>
+
  </div>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Modeling">Modeling</a>
+
  <div class="doubleColumn">
−
</div>
+
<a href="https://static.igem.org/mediawiki/2015/c/c0/Technion_Israel_2015_protocols_gelextraction.pdf" target="_blank">Gel Extraction</a></br></br>
−
</div>
+
 
−
<div class="main2">
+
<p>Transformation</br>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Team">Team</a>
+
<ol><li>
−
<span class="btn-bg2"></span>
+
<a href="https://static.igem.org/mediawiki/2015/9/91/Technion_Israel_2015_protocols_transformation.pdf" target="_blank"><i>E.coli</i></a>
−
<div class="sub2">
+
</li>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us">About&nbsp;Us</a>
+
<li>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Attributions">Attributions</a>
+
<a href="https://static.igem.org/mediawiki/2015/e/ef/Technion_iGEM_2015_B.Subtilis_Transformation.pdf" target="_blank"><i>B.subtilis</i></a></li>
−
<a href="https://2015.igem.org/Team:Technion_Israel/Team/Sponsors">Sponsors</a>
+
</ol></p>
−
</div>
+
−
</div>
+
−
<div class="main3">
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook">Lab&nbsp;Notebook</a>
+
−
<span class="btn-bg3"></span>
+
−
<div class="sub3">
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Overview">Overview</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols">Protocols</a>
+
−
</div>
+
−
</div>
+
−
<div class="main4">
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Practices">Human&nbsp;Practices</a>
+
−
<span class="btn-bg4"></span>
+
−
<div class="sub4">
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Practices/Education">Education</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Practices/Publications">Publications</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Practices/Market_Research">Market&nbsp;Research</a>
+
−
</div>
+
−
</div>
+
−
<div class="main5">
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria">Judging&nbsp;Criteria</a>
+
−
<span class="btn-bg5"></span>
+
−
<div class="sub5">
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Parts">Parts</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria/Achievements">Achievements</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Collaborations">Collaborations</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Design">Design</a>
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Entrepreneurship">Entrepreneurship</a>
+
−
</div>
+
−
</div>
+
−
</nav>
+
−
</div>
+
−
<!---------------------------------------dropdown menu-end----------------------------------->
+
  
−
<div id="protocol_instructions">
+
<p>Colony PCR</br>
−
<p>Here you can find all the final protocols we worked with for out project. Click the main protocol category to be linked to the webpage containing the protocols in that category. There, you will have the option to download PDFs of each protocol.</p>
+
<ol><li><a href="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_protocols_colonyPCR.pdf" target="_blank"><i>E. coli</i></a></li>
 +
<li><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf" target="_blank"><i>B. subtilis</i></a></li></ol></p>
 +
<a href="https://static.igem.org/mediawiki/2015/c/c8/Technion_Israel_2015_protocols_ligation.pdf" target="_blank">Ligation</a></br></br>
 +
<a href="https://static.igem.org/mediawiki/2015/a/ab/Technion_Israel_2015_protocols_nanoview.pdf" target="_blank">Nanodrop Use</a></br></br>
 +
  </div>
 
</div>
 
</div>
−
<div class= "Protocol_Main">
+
 
−
<div id="DNA_protocols">
+
</div>
−
<img src="https://static.igem.org/mediawiki/2015/6/64/Technion_Israel_2015_DNA.png" alt="DNA" height="200" width="200" >
+
<div class="DNA">
−
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols/Cloning"><h1>DNA and Cloning</h1></a>
+
<div class="mini-title2">
−
</div>
+
−
<div id= "Proof_protocols">
+
−
<img src="https://static.igem.org/mediawiki/2015/7/7f/Technion_Israel_2015_Protocols_i.png" alt="Medium" height="150" width="50" >
+
−
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols/Proof"><h1>The Works: Proof of Concept</h1></a>
+
 
</div>
 
</div>
−
<div id="Comb_protocols">
+
<hr></hr>
−
<img src="https://static.igem.org/mediawiki/2015/1/1a/Technion_Israel_2015_Protocols_Comb.png" alt="Medium" height="200" width="200" >
+
<div class="doubleColumns">
−
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols/Comb"><h1>Comb Experiments</h1></a>
+
  <div class="doubleColumn">
 +
<a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><i>Bacillus</i> Secretion Verification</a>
 +
</br></br><a href="https://static.igem.org/mediawiki/2015/7/7e/Technion_Israel_2015_protocols_3aHSDactivity.pdf" target="_blank">3ɑ-HSD Activity Verification Protocol</a>
 +
</br></br><a href="https://static.igem.org/mediawiki/2015/2/22/Technion_Israel_2015_protocol_NADPHfluorescence.pdf" target="_blank">Extracellular NADPH Estimation</a></br></br>
 +
<a href="https://static.igem.org/mediawiki/2015/4/47/Technion_Israel_2015_newLysisMethodComparison.pdf" target="_blank">Comparison of Lysis Procedures for NADPH Measurement</a>
 +
</br></br><a href="https://static.igem.org/mediawiki/2015/1/14/Technion_Israel_2015_protocols_shelf-life.pdf" target="_blank">Shelf-life and Storage Conditions Estimation</a>
 +
  </div>
 +
 
 
</div>
 
</div>
−
<div id= "Medium_protocols">
 
−
<img src="https://static.igem.org/mediawiki/2015/e/e0/Technion_Israel_2015_Protocols_Methods.png" alt="Medium" height="150" width="100" >
 
−
<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols/Mediums"><h1>Medium Preparation</h1></a>
 
 
</div>
 
</div>
−
<div id= "Misc_protocols">
 
−
<img src="https://static.igem.org/mediawiki/2015/8/8a/Technion_Israel_2015_Protocols_Bacteria.png" alt="Medium" height="500" width="450">
 
 
</div>
 
</div>
−
</div>
+
 
 +
<footer>
 +
<div class="team_name">
 +
<h3>Contact Us</h3>
 +
</div>
 +
<nav class="connect_links">
 +
<div class="facebook_link">
 +
<a href="https://www.facebook.com/technioniGEM2015?pnref=story" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2015/0/06/Team_Technion_Footer_facebook.png" alt="Follow us on facebook" /></a>
 +
</div>
 +
<div class="email_link">
 +
<a href="mailto:igem.technion2015@gmail.com">
 +
<img src="https://static.igem.org/mediawiki/2015/d/da/Team_Technion_Footer_gmail.png" alt="Send us an Email" /></a>
 +
</div>
 +
</nav>
 +
</footer>
 +
 
 +
<script type="text/javascript" src="https://2015.igem.org/Template:Technion/navbar/javascript?
 +
action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2015.igem.org/Template:Technion/Javascript?
 +
action=raw&ctype=text/javascript"></script>
 +
</body>
 
</html>
 
</html>

Latest revision as of 12:24, 18 September 2015

Team: Technion 2015

Home
Home
Project
Lab Notebook
Human Practices
Judging Criteria
Team
UP
Scroll to top

Protocols

Gel Preparation

Sigma-Aldrich Miniprep

Primer Dilution

CIP

Gibson Assembly

Restriction

UV Gel Viewing

Gel Extraction

Transformation

  1. E.coli
  2. B.subtilis

Colony PCR

  1. E. coli
  2. B. subtilis

Ligation

Nanodrop Use

Bacillus Secretion Verification

3ɑ-HSD Activity Verification Protocol

Extracellular NADPH Estimation

Comparison of Lysis Procedures for NADPH Measurement

Shelf-life and Storage Conditions Estimation

Contact Us