Difference between revisions of "Team:Technion Israel/Lab Notebook/Protocols"

 
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<h1><b>Protocols</b></h1>
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<div class="DNA">
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<a href="https://static.igem.org/mediawiki/2015/c/c0/Technion_Israel_2015_protocols_gelextraction.pdf" target="_blank">Gel Extraction</a></br></br>
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<p>Transformation</br>
 +
<ol><li>
 +
<a href="https://static.igem.org/mediawiki/2015/9/91/Technion_Israel_2015_protocols_transformation.pdf" target="_blank"><i>E.coli</i></a>
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</li>
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<li>
 +
<a href="https://static.igem.org/mediawiki/2015/e/ef/Technion_iGEM_2015_B.Subtilis_Transformation.pdf" target="_blank"><i>B.subtilis</i></a></li>
 +
</ol></p>
 +
 +
<p>Colony PCR</br>
 +
<ol><li><a href="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_protocols_colonyPCR.pdf" target="_blank"><i>E. coli</i></a></li>
 +
<li><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf" target="_blank"><i>B. subtilis</i></a></li></ol></p>
 +
<a href="https://static.igem.org/mediawiki/2015/c/c8/Technion_Israel_2015_protocols_ligation.pdf" target="_blank">Ligation</a></br></br>
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<a href="https://static.igem.org/mediawiki/2015/a/ab/Technion_Israel_2015_protocols_nanoview.pdf" target="_blank">Nanodrop Use</a></br></br>
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<a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><i>Bacillus</i> Secretion Verification</a>
 +
</br></br><a href="https://static.igem.org/mediawiki/2015/7/7e/Technion_Israel_2015_protocols_3aHSDactivity.pdf" target="_blank">3ɑ-HSD Activity Verification Protocol</a>
 +
</br></br><a href="https://static.igem.org/mediawiki/2015/2/22/Technion_Israel_2015_protocol_NADPHfluorescence.pdf" target="_blank">Extracellular NADPH Estimation</a></br></br>
 +
<a href="https://static.igem.org/mediawiki/2015/4/47/Technion_Israel_2015_newLysisMethodComparison.pdf" target="_blank">Comparison of Lysis Procedures for NADPH Measurement</a>
 +
</br></br><a href="https://static.igem.org/mediawiki/2015/1/14/Technion_Israel_2015_protocols_shelf-life.pdf" target="_blank">Shelf-life and Storage Conditions Estimation</a>
 +
  </div>
  
<div class= "Protocol_Main">
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</div>
<div class="protocol_list">
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</div>
<div class="four_columns">
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</div>
<div id="pic_cloning">
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<img style="border-top: 5px solid black; border-bottom: 5px solid black" src="https://static.igem.org/mediawiki/2015/4/47/DNA.png" alt="DNA and Cloning" width="900px" height="300px">
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</div>
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<div class="wrapper">
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<div class="cloning_left">
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<ul>
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<li>
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<h2>Gel Extraction</h2>
+
</li>
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<li>
+
<h2>Sigma-Aldrich Miniprep</h2>
+
<li>
+
<h2>Phusion PCR</h2>
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</li>
+
<li>
+
<h2>Electroporation</h2>
+
<ol>
+
<h3><i>E.coli</i></h3>
+
</ol>
+
<ol>
+
<a href="https://static.igem.org/mediawiki/2015/e/ef/Technion_iGEM_2015_B.Subtilis_Transformation.pdf"><h3><i>B.Subtilis</i></h3></a>
+
</ol>
+
</li>
+
</ul>
+
</div>
+
<div class="cloning_left">
+
<ul>
+
<li>
+
<h2>Transformation</h2>
+
<ol>
+
<h3><i>E.coli</i></h3>
+
</ol>
+
<ol>
+
<h3><i>B.Subtilis</i></h3>
+
</ol>
+
</li>
+
<li>
+
<h2>Colony PCR</h2>
+
<ol><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf"><h3><i>B. Subtilis</i></h3></a>
+
</ol>
+
<li>
+
<h2>Preparing Competent <i>E.coli</i></h2>
+
</li>
+
<li>
+
<h2>DNA Gel Electrophoresis</h2>
+
</li>
+
<li>
+
<h2>Preparation of DNA for Sequencing</h2>
+
</li>
+
</ul>
+
</div>
+
</div>
+
</div>
+
<div class="four_columns" id="Proof">
+
<div id="pic_proof">
+
<img style="border-top: 5px solid black; border-bottom: 5px solid black" src="https://static.igem.org/mediawiki/2015/6/66/Technion_Israel_2015_Protocols_Proof.jpg" alt="Proof of Concept" width="900px" height="300px">
+
</div>
+
<div class="wrapper_proof">
+
<div class="cloning_left">
+
<ul>
+
<li><a href="https://static.igem.org/mediawiki/2015/4/4b/Technion_Israel_2015_LysisMethodComparison.pdf" target="_blank"><h2>Comparison of Lysis Procedures</h2></a>
+
</li>
+
</ul>
+
</div>
+
</div>
+
</div>
+
  
<div class="four_columns" id="comb">
 
<div id="pic_comb">
 
<img style="border-top: 5px solid black; border-bottom: 5px solid black" src="https://static.igem.org/mediawiki/2015/7/70/Technion_iGEM_2015_Comb.jpg" alt="Comb" width="900px" height="300px">
 
</div>
 
</div>
 
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<img style="border-top: 5px solid black; border-bottom: 5px solid black" src="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_Protocols_Misc.jpg" alt="Miscellaneous" width="900px" height="300px"></a>
 
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<ul>
 
<li><a href="https://static.igem.org/mediawiki/2015/b/bb/Technion_iGEM_2015_Primer_dilution_protocol.pdf" target="_blank"><h2>Primer Dilution</h2></a></li>
 
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Latest revision as of 12:24, 18 September 2015

Team: Technion 2015

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