Difference between revisions of "Team:Technion Israel/Lab Notebook/Protocols"
(32 intermediate revisions by 3 users not shown) | |||
Line 5: | Line 5: | ||
<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Technion/CSS?action=raw&ctype=text/css" /> | <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Technion/CSS?action=raw&ctype=text/css" /> | ||
− | + | <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Technion/navbar/css?action=raw&ctype=text/css" /> | |
<style type="text/css"> | <style type="text/css"> | ||
body { | body { | ||
background-color: rgb(202, 174, 244); | background-color: rgb(202, 174, 244); | ||
} | } | ||
− | + | header{background-image: url("https://static.igem.org/mediawiki/2015/2/28/Technion_Israel_2015_protocols.jpg"); background-size: 100%; background-repeat: no-repeat; width: 100%; height: 400px; margin-bottom: 0px;} | |
− | + | .title h1{ color: rgb(202, 174, 244); font-weight: bold; font-size: 100px; text-align: center; background: rgba(0, 0, 0, 0.8); font-family: sans-serif; font-style: Arial; height: 100px; text-shadow: black 6px 6px 6px; line-height: 80px;} | |
− | + | #bodyContent h1{margin-bottom: 0em;} | |
− | + | .mini-title{background-image: url("https://static.igem.org/mediawiki/2015/9/96/Technion_Israel_2015_dna.jpg"); background-size: 100%; background-repeat: no-repeat; width: 100%; height: 200px; margin-bottom: 0px;} | |
− | padding: 20px; | + | .content{padding: 20px; margin-top: 0px; font-size: 16px;} |
− | + | .mini-title2{background-image: url("https://static.igem.org/mediawiki/2015/b/b6/Technion_Israel_2015_proofof.jpg"); background-size: 100%; background-repeat: no-repeat; width: 100%; height: 200px; margin-bottom: 0px;} | |
− | + | ||
− | + | ||
− | + | .doubleColumns{ | |
− | + | display:table; | |
− | + | border-spacing:12px; | |
− | + | min-height:200px; | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | min- | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
width: 100%; | width: 100%; | ||
− | + | margin: auto; | |
− | + | ||
} | } | ||
− | . | + | .doubleColumn{ |
− | + | display:table-cell; | |
− | + | width:48%; padding-left: 150px; | |
− | + | ||
− | + | ||
− | width: | + | |
− | + | ||
− | + | ||
} | } | ||
− | . | + | .DNA{display: block; width: 100%;} |
− | + | .up_button img { width: 130px;} | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | } | + | |
− | + | ||
− | . | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | } | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</style> | </style> | ||
− | <title>Team: Technion 2015</title> | + | <title>Team: Technion 2015</title> |
</head> | </head> | ||
<body> | <body> | ||
− | <!--------------------------------------- | + | <!-------------------------------------nav-bar----------------------------------------------> |
− | <div class=" | + | <div class="nav_menu"> |
− | + | ||
− | <img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id=" | + | <a href="https://2015.igem.org/Team:Technion_Israel" style="position:fixed; width: 170px; display:inline-block; text-decoration: none;" class="nav_home_link"> |
− | + | <img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id="nav_home" /><div class="home_text">Home</div></a> | |
− | + | ||
− | + | <div class="main_buttons"> | |
− | + | <div id="project" class="main_button">Project</div> | |
− | + | <div id="Lab_Notebook" class="main_button">Lab Notebook</div> | |
− | + | <div id="Human_Practices" class="main_button">Human Practices</div> | |
− | + | <div id="Judging_Criteria" class="main_button">Judging Criteria</div> | |
− | + | <div id="Team" class="main_button">Team</div> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
− | <!-------------------------------------- | + | <nav> |
+ | <div id="Team_Links" class="main_links"> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us" id="Team_Links_About_Us">About Us</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Attributions" id="Team_Links_Attributions">Attributions</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Team/Sponsors" id="Team_Links_Sponsors">Sponsors</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Team/gallery" id="Team_Links_gallery">Gallery</a> | ||
+ | </div> | ||
+ | <div id="Judging_Criteria_Links" class="main_links"> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Parts" id="Judging_Criteria_Links_Parts">Parts</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria/Achievements" id="Judging_Criteria_Links_Achievements">Achievements</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Collaborations" id="Judging_Criteria_Links_Collaborations">Collaborations</a> | ||
+ | </div> | ||
+ | <div id="Human_Practices_Links" class="main_links"> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Practices">Practices</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Practices/safety">Safety</a> | ||
+ | </div> | ||
+ | <div id="Lab_Notebook_Links" class="main_links"> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Overview">Overview</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols">Protocols</a> | ||
+ | </div> | ||
+ | <div id="project_Links" class="main_links"> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Overview">Overview</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Secretion">Secretion</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Expression">Expression</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Cofactor">Cofactor</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Design">Comb</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Modeling">Modeling</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Results">Results</a> | ||
+ | </div> | ||
+ | </nav> | ||
+ | </div> | ||
+ | <!-------------------------------------nav-bar-end------------------------------------------> | ||
− | + | <!-------------------------------------up-button--------------------------------------------> | |
− | + | <div class="up_button" style="text-decoration: none;"> | |
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <a href="#" class="up_link" style="text-decoration: none;"> |
+ | <div class="up_button_text" style="text-decoration: none;">UP</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/7/7a/Technion_2015_Up_hippie.png" alt="Scroll to top" /> | ||
+ | </a> | ||
+ | </div> | ||
+ | <!-------------------------------------up-button-end----------------------------------------> | ||
+ | <header> | ||
</header> | </header> | ||
− | + | <div class="title"> | |
+ | <h1><b>Protocols</b></h1> | ||
+ | </div> | ||
− | <div class=" | + | <div class= "content"> |
− | + | <div class="DNA"> | |
− | + | <div class="mini-title"> | |
− | + | </div> | |
− | + | <hr></hr> | |
− | + | <div class="doubleColumns"> | |
− | + | <div class="doubleColumn"> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/b/b8/Techion_Israel_2015_protocols_gelpreparation.pdf" target="_blank">Gel Preparation</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/b/ba/Technion_Israel_2015_protocols_miniprep.pdf" target="_blank">Sigma-Aldrich Miniprep</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/b/bb/Technion_iGEM_2015_Primer_dilution_protocol.pdf" target="_blank">Primer Dilution</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/c/cb/Technion_Israel_2015_protocols_CIP.pdf" target="_blank">CIP</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/2/26/Technion_Israel_2015_protocols_gibson.pdf" target="_blank">Gibson Assembly</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/2/23/Technion_Israel_2015_protocols_restriction.pdf" target="_blank">Restriction</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/1/1d/Technion_Israel_2015_protocols_UVviewing.pdf" target="_blank">UV Gel Viewing</a></br></br> | |
− | + | ||
− | < | + | </div> |
− | + | <div class="doubleColumn"> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/c/c0/Technion_Israel_2015_protocols_gelextraction.pdf" target="_blank">Gel Extraction</a></br></br> | |
− | + | ||
− | + | <p>Transformation</br> | |
+ | <ol><li> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/9/91/Technion_Israel_2015_protocols_transformation.pdf" target="_blank"><i>E.coli</i></a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/e/ef/Technion_iGEM_2015_B.Subtilis_Transformation.pdf" target="_blank"><i>B.subtilis</i></a></li> | ||
+ | </ol></p> | ||
− | + | <p>Colony PCR</br> | |
− | + | <ol><li><a href="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_protocols_colonyPCR.pdf" target="_blank"><i>E. coli</i></a></li> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf" target="_blank"><i>B. subtilis</i></a></li></ol></p> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/c/c8/Technion_Israel_2015_protocols_ligation.pdf" target="_blank">Ligation</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/a/ab/Technion_Israel_2015_protocols_nanoview.pdf" target="_blank">Nanodrop Use</a></br></br> | |
− | + | </div> | |
− | + | </div> | |
− | + | ||
− | + | </div> | |
− | + | <div class="DNA"> | |
− | + | <div class="mini-title2"> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
− | < | + | <hr></hr> |
− | + | <div class="doubleColumns"> | |
− | <div class=" | + | <div class="doubleColumn"> |
− | + | <a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><i>Bacillus</i> Secretion Verification</a> | |
− | + | </br></br><a href="https://static.igem.org/mediawiki/2015/7/7e/Technion_Israel_2015_protocols_3aHSDactivity.pdf" target="_blank">3ɑ-HSD Activity Verification Protocol</a> | |
− | < | + | </br></br><a href="https://static.igem.org/mediawiki/2015/2/22/Technion_Israel_2015_protocol_NADPHfluorescence.pdf" target="_blank">Extracellular NADPH Estimation</a></br></br> |
− | + | <a href="https://static.igem.org/mediawiki/2015/4/47/Technion_Israel_2015_newLysisMethodComparison.pdf" target="_blank">Comparison of Lysis Procedures for NADPH Measurement</a> | |
− | + | </br></br><a href="https://static.igem.org/mediawiki/2015/1/14/Technion_Israel_2015_protocols_shelf-life.pdf" target="_blank">Shelf-life and Storage Conditions Estimation</a> | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<footer> | <footer> | ||
<div class="team_name"> | <div class="team_name"> | ||
Line 457: | Line 167: | ||
</nav> | </nav> | ||
</footer> | </footer> | ||
− | </ | + | |
+ | <script type="text/javascript" src="https://2015.igem.org/Template:Technion/navbar/javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2015.igem.org/Template:Technion/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 12:24, 18 September 2015