Difference between revisions of "Team:Technion Israel/Lab Notebook/Protocols"
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− | <title>Team: Technion 2015</title> | + | <title>Team: Technion 2015</title> |
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− | <a href="https://2015.igem.org/Team:Technion_Israel"> | + | <a href="https://2015.igem.org/Team:Technion_Israel" style="position:fixed; width: 170px; display:inline-block; text-decoration: none;" class="nav_home_link"> |
− | <img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id="nav_home" /></a> | + | <img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id="nav_home" /><div class="home_text">Home</div></a> |
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− | <a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us">About Us</a> | + | <a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us" id="Team_Links_About_Us">About Us</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/Attributions">Attributions</a> | + | <a href="https://2015.igem.org/Team:Technion_Israel/Attributions" id="Team_Links_Attributions">Attributions</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/Team/Sponsors">Sponsors</a> | + | <a href="https://2015.igem.org/Team:Technion_Israel/Team/Sponsors" id="Team_Links_Sponsors">Sponsors</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/Team/gallery">Gallery</a> | + | <a href="https://2015.igem.org/Team:Technion_Israel/Team/gallery" id="Team_Links_gallery">Gallery</a> |
</div> | </div> | ||
<div id="Judging_Criteria_Links" class="main_links"> | <div id="Judging_Criteria_Links" class="main_links"> | ||
− | <a href="https://2015.igem.org/Team:Technion_Israel/Parts">Parts</a> | + | <a href="https://2015.igem.org/Team:Technion_Israel/Parts" id="Judging_Criteria_Links_Parts">Parts</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria/Achievements">Achievements</a> | + | <a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria/Achievements" id="Judging_Criteria_Links_Achievements">Achievements</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/Collaborations" | + | <a href="https://2015.igem.org/Team:Technion_Israel/Collaborations" id="Judging_Criteria_Links_Collaborations">Collaborations</a> |
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<div id="project_Links" class="main_links"> | <div id="project_Links" class="main_links"> | ||
<a href="https://2015.igem.org/Team:Technion_Israel/Project/Overview">Overview</a> | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Overview">Overview</a> | ||
− | <a href="https://2015.igem.org/Team:Technion_Israel/Project/ | + | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Secretion">Secretion</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/Project/ | + | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Expression">Expression</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/Project/ | + | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Cofactor">Cofactor</a> |
− | <a href="https://2015.igem.org/Team:Technion_Israel/ | + | <a href="https://2015.igem.org/Team:Technion_Israel/Design">Comb</a> |
<a href="https://2015.igem.org/Team:Technion_Israel/Modeling">Modeling</a> | <a href="https://2015.igem.org/Team:Technion_Israel/Modeling">Modeling</a> | ||
+ | <a href="https://2015.igem.org/Team:Technion_Israel/Project/Results">Results</a> | ||
</div> | </div> | ||
</nav> | </nav> | ||
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− | <div class="up_button"> | + | <div class="up_button" style="text-decoration: none;"> |
− | <a href="#" class="up_link"> | + | <a href="#" class="up_link" style="text-decoration: none;"> |
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <div class="up_button_text" style="text-decoration: none;">UP</div> |
+ | <img src="https://static.igem.org/mediawiki/2015/7/7a/Technion_2015_Up_hippie.png" alt="Scroll to top" /> | ||
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</header> | </header> | ||
− | + | <div class="title"> | |
+ | <h1><b>Protocols</b></h1> | ||
+ | </div> | ||
− | <div class=" | + | <div class= "content"> |
− | + | <div class="DNA"> | |
− | + | <div class="mini-title"> | |
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− | + | <a href="https://static.igem.org/mediawiki/2015/b/b8/Techion_Israel_2015_protocols_gelpreparation.pdf" target="_blank">Gel Preparation</a></br></br> | |
− | < | + | <a href="https://static.igem.org/mediawiki/2015/b/ba/Technion_Israel_2015_protocols_miniprep.pdf" target="_blank">Sigma-Aldrich Miniprep</a></br></br> |
− | + | <a href="https://static.igem.org/mediawiki/2015/b/bb/Technion_iGEM_2015_Primer_dilution_protocol.pdf" target="_blank">Primer Dilution</a></br></br> | |
+ | <a href="https://static.igem.org/mediawiki/2015/c/cb/Technion_Israel_2015_protocols_CIP.pdf" target="_blank">CIP</a></br></br> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/2/26/Technion_Israel_2015_protocols_gibson.pdf" target="_blank">Gibson Assembly</a></br></br> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/2/23/Technion_Israel_2015_protocols_restriction.pdf" target="_blank">Restriction</a></br></br> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/1/1d/Technion_Israel_2015_protocols_UVviewing.pdf" target="_blank">UV Gel Viewing</a></br></br> | ||
+ | |||
+ | </div> | ||
+ | <div class="doubleColumn"> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/c/c0/Technion_Israel_2015_protocols_gelextraction.pdf" target="_blank">Gel Extraction</a></br></br> | ||
+ | |||
+ | <p>Transformation</br> | ||
+ | <ol><li> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/9/91/Technion_Israel_2015_protocols_transformation.pdf" target="_blank"><i>E.coli</i></a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/e/ef/Technion_iGEM_2015_B.Subtilis_Transformation.pdf" target="_blank"><i>B.subtilis</i></a></li> | ||
+ | </ol></p> | ||
− | + | <p>Colony PCR</br> | |
− | + | <ol><li><a href="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_protocols_colonyPCR.pdf" target="_blank"><i>E. coli</i></a></li> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf" target="_blank"><i>B. subtilis</i></a></li></ol></p> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/c/c8/Technion_Israel_2015_protocols_ligation.pdf" target="_blank">Ligation</a></br></br> | |
− | + | <a href="https://static.igem.org/mediawiki/2015/a/ab/Technion_Israel_2015_protocols_nanoview.pdf" target="_blank">Nanodrop Use</a></br></br> | |
− | + | </div> | |
+ | </div> | ||
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− | <div class=" | + | <div class="doubleColumn"> |
− | + | <a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><i>Bacillus</i> Secretion Verification</a> | |
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</br></br><a href="https://static.igem.org/mediawiki/2015/7/7e/Technion_Israel_2015_protocols_3aHSDactivity.pdf" target="_blank">3ɑ-HSD Activity Verification Protocol</a> | </br></br><a href="https://static.igem.org/mediawiki/2015/7/7e/Technion_Israel_2015_protocols_3aHSDactivity.pdf" target="_blank">3ɑ-HSD Activity Verification Protocol</a> | ||
− | + | </br></br><a href="https://static.igem.org/mediawiki/2015/2/22/Technion_Israel_2015_protocol_NADPHfluorescence.pdf" target="_blank">Extracellular NADPH Estimation</a></br></br> | |
+ | <a href="https://static.igem.org/mediawiki/2015/4/47/Technion_Israel_2015_newLysisMethodComparison.pdf" target="_blank">Comparison of Lysis Procedures for NADPH Measurement</a> | ||
+ | </br></br><a href="https://static.igem.org/mediawiki/2015/1/14/Technion_Israel_2015_protocols_shelf-life.pdf" target="_blank">Shelf-life and Storage Conditions Estimation</a> | ||
+ | </div> | ||
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</div> | </div> | ||
</div> | </div> | ||
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<footer> | <footer> | ||
<div class="team_name"> | <div class="team_name"> |
Latest revision as of 12:24, 18 September 2015