Difference between revisions of "Team:IIT Madras/Parts"

 
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<h2>Sender : <em>E. coli</em></h2>
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<h2>Pathogen Model : <em>E. coli</em></h2>
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<div align="center"><img height="370px" width="370px" src = "https://static.igem.org/mediawiki/2015/8/8c/Sender_plasmid_A.png"></div>
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<br></br>
 
<br></br>
  
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<h2>Receiver : <em>L. lactis</em> </h2>
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<h2>Our System : <em>L. lactis</em> </h2>
  
  
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<img align="left" height="350px" width="350px" src="https://static.igem.org/mediawiki/2015/9/9c/Reciever_l_lactis_plasmid_a.png" >
 
<img align="left" height="350px" width="350px" src="https://static.igem.org/mediawiki/2015/9/9c/Reciever_l_lactis_plasmid_a.png" >
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<img align="right" height="380px" width="3805px" src="https://static.igem.org/mediawiki/2015/1/1b/Reciever_l_lactis_plasmid_b.png" >
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<img align="right" height="385px" width="385px" src="https://static.igem.org/mediawiki/2015/1/1b/Reciever_l_lactis_plasmid_b.png" >
 
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<h2>Used Parts  : </h2>
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<table style="width:100%">
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  <tr>
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    <td align="center"><b>PART NAME</b></td>
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    <td align="center"><b>PART NUMBER</b></td>
 +
  </tr>
 +
  <tr>
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    <td>LuxPQ followed by terminator and LuxOU generator</td>
 +
    <td align="center"><a href = "http://parts.igem.org/Part:BBa_K218006">BBa_K218006</a></td>
 +
  </tr>
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  <tr>
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    <td>AI-2 Generator (LuxS-Pfs)</td>
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    <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1202110">BBa_K1202110</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>rpoN: coding sigma 54 factor</td>
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    <td align="center"><a href = "http://parts.igem.org/Part:BBa_K622000">BBa_K622000</a></td>
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  </tr>
 +
  <tr>
 +
    <td>Promoter CP44</td>
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    <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1033225">BBa_K1033225</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>double terminator (B0010-B0012)</td>
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    <td align="center"><a href = "http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></td>
 +
  </tr>
 +
 
 +
</table>
 +
<br></br>
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 +
<h2>Parts to be submitted : </h2>
 
<table style="width:100%">
 
<table style="width:100%">
 
   <tr>
 
   <tr>
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   <tr>
 
   <tr>
 
     <td>LuxR (vibrio harveyi) inducible promoter</td>
 
     <td>LuxR (vibrio harveyi) inducible promoter</td>
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     <td>BBa_K1654000</td>  
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     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654000">BBa_K1654000</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>LuxR (vibrio harveyi) repressible promoter</td>
 
     <td>LuxR (vibrio harveyi) repressible promoter</td>
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     <td>BBa_K1654001</td>  
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     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654001">BBa_K1654001</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>LuxR (vibrio harveyi) protein</td>
 
     <td>LuxR (vibrio harveyi) protein</td>
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     <td>BBa_K1654002</td>  
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     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654002">BBa_K1654002</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>Hfq coding region</td>
 
     <td>Hfq coding region</td>
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     <td>BBa_K1654003</td>  
+
     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654003">BBa_K1654003</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>LuxO-P and sigma54 inducible promoter</td>
 
     <td>LuxO-P and sigma54 inducible promoter</td>
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     <td>BBa_K1654004</td>  
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     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654004">BBa_K1654004</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>LuxR (vibrio harveyi) induced expression of Alyteserin-1a</td>
 
     <td>LuxR (vibrio harveyi) induced expression of Alyteserin-1a</td>
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     <td>BBa_K1654005</td>  
+
     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654005">BBa_K1654005</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>USP45 secretion tag for Lactococcus lactis</td>
 
     <td>USP45 secretion tag for Lactococcus lactis</td>
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     <td>BBa_K1654007</td>  
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     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654007">BBa_K1654007</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>Alyteserin-1a coding</td>
 
     <td>Alyteserin-1a coding</td>
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     <td>BBa_K1654009</td>  
+
     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654009">BBa_K1654009</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>Naly (neutralizing Alyteserin-1a) coding</td>
 
     <td>Naly (neutralizing Alyteserin-1a) coding</td>
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     <td>BBa_K1654010</td>  
+
     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654010">BBa_K1654010</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td>LuxR (vibrio harveyi ) repressible expression of Naly</td>
+
     <td>LuxR (vibrio harveyi) repressible expression of Naly</td>
−
     <td>BBa_K1654011</td>  
+
     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654011">BBa_K1654011</a></td>  
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>LuxR expression system</td>
 
     <td>LuxR expression system</td>
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     <td>BBa_K1654012</td>  
+
     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654012">BBa_K1654012</a></td>  
 
   </tr>  
 
   </tr>  
 
   <tr>
 
   <tr>
 
     <td>Expression system for Hfq</td>
 
     <td>Expression system for Hfq</td>
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     <td>BBa_K1654013</td>  
+
     <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654013">BBa_K1654013</a></td>  
 
   </tr>
 
   </tr>
 +
  <tr>
 +
    <td>Constitutive expression of sigma54 in L. lactis</td>
 +
    <td align="center"><a href = "http://parts.igem.org/Part:BBa_K1654014">BBa_K1654014</a></td>
 +
  </tr>
 +
  
  
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</body>
 
</body>
 
</html>
 
</html>
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{{Team:IIT_Madras_Footer_Final}}

Latest revision as of 13:06, 18 September 2015

Pathogen Model : E. coli



Our System : L. lactis

Used Parts :

PART NAME PART NUMBER
LuxPQ followed by terminator and LuxOU generator BBa_K218006
AI-2 Generator (LuxS-Pfs) BBa_K1202110
rpoN: coding sigma 54 factor BBa_K622000
Promoter CP44 BBa_K1033225
double terminator (B0010-B0012) BBa_B0015


Parts to be submitted :

PART NAME PART NUMBER
LuxR (vibrio harveyi) inducible promoter BBa_K1654000
LuxR (vibrio harveyi) repressible promoter BBa_K1654001
LuxR (vibrio harveyi) protein BBa_K1654002
Hfq coding region BBa_K1654003
LuxO-P and sigma54 inducible promoter BBa_K1654004
LuxR (vibrio harveyi) induced expression of Alyteserin-1a BBa_K1654005
USP45 secretion tag for Lactococcus lactis BBa_K1654007
Alyteserin-1a coding BBa_K1654009
Naly (neutralizing Alyteserin-1a) coding BBa_K1654010
LuxR (vibrio harveyi) repressible expression of Naly BBa_K1654011
LuxR expression system BBa_K1654012
Expression system for Hfq BBa_K1654013
Constitutive expression of sigma54 in L. lactis BBa_K1654014

About Us

We are a team of undergraduates from the Department of Biotechnology, IIT Madras in iGEM 2015

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Location

Department of Biotechnology, IIT Madras Sardar Patel Road, Chennai
Pincode : 600036

Contact Us

Email: igemiitm2015@gmail.com
Tel: +04422574128